Table 1

Purification of PGC2 from culture of FOC4 grown on SM supplemented with 1% citrus pectin as the sole carbon source

Step

Total protein

(mg)

Total activity

(Unit)^a

Yield

(%)

Specific activity

(Units mg protein^-1min^-1)

Crude

36.60

131.25

100

3.59

Ultrafiltration

8.21

70.26

53.53

8.56

Sephacryl S-100 16/60

1.85

20.35

15.51

11

Sepharose FF CM Hitrap

0.26

5.54

4.22

21.30

^a One unit of PG activity was defined as the amount of enzyme that liberated one μmole of GA in one minute at 45°C.

Dong and Wang BMC Biochemistry 2011 12:51   doi:10.1186/1471-2091-12-51