Table 1

Zinc:Protein Ratio for CND and bZDD

RAG1 Domain

Conditions

Zinc:Protein Ratio ICP-MSa,b

Zinc:Protein Ratio FAASa,b


CNDc

Zinc Free Buffer

2.08 ± 0.68

2.00 ± 0.44

CNDc

DTPA Buffer

0.24 ± 0.14

0.37 ± 0.29

bZDDd

Zinc Free Buffer

4.23 ± 0.65

4.12 ± 0.78


a The zinc content of each construct was determined using ICP-MS and FAAS.

b Data presented represent the average of two or more independent experiments.

c Zinc binding results for mbp-CND and tagless CND were comparable. The results presented for CND represent the average from both data sets.

d Zinc binding for bZDD was evaluated for the mbp-tagged construct only.

Arbuckle et al. BMC Biochemistry 2011 12:23   doi:10.1186/1471-2091-12-23

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