Figure 2.

Acetaldehyde induced modification and inactivation of BSL-B. A) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of B. subtilis lipase B (BSL-B) treated with acetaldehyde (0-0.5 M) in glycine/NaOH buffer (10 mM, pH 10). S: Molecular weight standard. Arrows indicate putative lipase dimers (~ 40 kDa) and trimers (~ 60 kDa). B) Soluble protein concentration and residual hydrolytic activity of BSL-B after treatment for 24 h with acetaldehyde (0-1.8 M) in glycine/NaOH buffer (10 mM, pH 10). C) Isoelectric focusing (IEF) gel (pH 3-10) of BSL-B after treatment with (0.5 M) and without acetaldehyde in glycine/NaOH (10 mM, pH 10). M: IEF marker. -: BSL-B without acetaldehyde. +: BSL-B with acetaldehyde.

Franken et al. BMC Biochemistry 2011 12:10   doi:10.1186/1471-2091-12-10
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