Chitinase and chitosanase activities of LA-a and LA-b under various pH conditions. (A) Colorimetric chitinase assay using CM-chitin-RBV as a substrate. Increase in hydrolyzed chitin was monitored by absorbance at 550 nm. (B) Fluorometric chitosanase assay using soluble chitosan as a substrate. Reaction product glucosamine (GlcN) was labeled with fluorescamine and monitored fluorometrically (excitation: 395 nm; emission: 493 nm). Graph shows average ± SD (n = 5).
Kitajima et al. BMC Biochemistry 2010 11:6 doi:10.1186/1471-2091-11-6