Purification of LA-a and LA-b. (A) SDS-PAGE of soluble fraction of mulberry latex obtained from petiole. Soluble proteins obtained from 0.025 μl of latex were loaded. (B) SDS-PAGE of purified LA-a and LA-b. One microgram of protein was loaded per lane. The proteins were stained with silver. (C and D) Hydrophobic interaction chromatography (phenyl sepharose, C) and cation-exchange chromatography (UNOS, D).
Kitajima et al. BMC Biochemistry 2010 11:6 doi:10.1186/1471-2091-11-6