Open Access Highly Accessed Methodology article

The DNA relaxation activity and covalent complex accumulation of Mycobacterium tuberculosis topoisomerase I can be assayed in Escherichia coli: application for identification of potential FRET-dye labeling sites

Gagandeep Narula, Jennifer Becker, Bokun Cheng, Neil Dani, Maria V Abrenica and Yuk-Ching Tse-Dinh*

Author Affiliations

Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA

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BMC Biochemistry 2010, 11:41  doi:10.1186/1471-2091-11-41

Published: 30 September 2010

Additional files

Additional file 1:

Quantitation of DNA nicking by MtTOP1-G116 S. The percent of nicked DNA in each lane of Figure 2A was quantitated by densitometry analysis. The increase in percent nicked DNA relative to the control lane with no enzyme present is shown here.

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