The DNA relaxation activity and covalent complex accumulation of Mycobacterium tuberculosis topoisomerase I can be assayed in Escherichia coli: application for identification of potential FRET-dye labeling sites
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
BMC Biochemistry 2010, 11:41 doi:10.1186/1471-2091-11-41Published: 30 September 2010
Additional file 1:
Quantitation of DNA nicking by MtTOP1-G116 S. The percent of nicked DNA in each lane of Figure 2A was quantitated by densitometry analysis. The increase in percent nicked DNA relative to the control lane with no enzyme present is shown here.
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