Figure 9.

NMR analysis of PfPMT-amodiaquine interaction. The glycine region of the 1H-15N HSQC spectra of PfPMT (0.3 mM), titrated with AQ (A) or CQ (B). The different colors indicate the inhibitor concentrations as follows: red- no inhibitor, orange- 1:4, yellow- 1:2, green- 1:1, blue- 2:1, violet- 3:1, black- 10:1. (C and D) Inhibitor titration plots for Gly32 and Gly68 (derived from A and B) showing the difference in binding between AQ and CQ. The overlay of the full HSQC spectrum in the absence or presence of AQ is shown in Additional File 1, Fig. S1.