Research article
Identification of inhibitors of Plasmodium falciparum phosphoethanolamine methyltransferase using an enzyme-coupled transmethylation assay
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* Corresponding author: Choukri B Mamoun choukri.benmamoun@yale.edu
1 Department of Internal Medicine, Section of Infectious Diseases, Yale School of Medicine, 333 Cedar St., New Haven, 06052, USA
2 Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, 06030, USA
3 The Program in Cell Biology, Department of Medicine, National Jewish Medical and Research Center, 1400 Jackson St, Denver, 80206, USA
4 Department of Molecular, Microbial, and Structural Biology University of Connecticut Health Center, 263 Farmington Ave., Farmington, 06030, USA
BMC Biochemistry 2010, 11:4 doi:10.1186/1471-2091-11-4
Published: 19 January 2010Additional files
Additional file 1:
Fig. S1. Overlay of the full 1H-15N HSQC spectra of PfPMT in the absence (red) or presence of 0.06 (orange), 0.12 (yellow), 0.25 (green), 0.5 (blue) and 1 mM (purple) of AQ.
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Additional file 2:
Fig. S2. Sequence alignment of HNMT and PfPMT. Residues that are identical, conserved, and semi-conserved are indicated by asterisk, colon, and period, respectively. The AQ-interacting residues are colored as in Fig. 8B. Phe19 and Tyr198 of HNMT and their corresponding residues in PfPMT are shown in italics and bold.
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