ATP hydrolysis by individual and pairwise Mcms and Mcm4/6/7 complexes. The mean rate of ATP hydrolysis for the indicated individual Mcms, pairwise combinations and Mcm4/6/7 complexes is shown. Rate measurements were determined at 1 mM ATP as described in "Methods". For the pairwise combinations of Mcm proteins, the rate is expressed per μg of the Mcm protein that is predicted to bind nucleotide within the pairs (as indicated). For comparison to Mcm4 + Mcm7, the rate of ATP hydrolysis by Mcm4/6/7 is expressed per μg Mcm4 (assuming equimolar ratios of each protein in the complex).
Ma et al. BMC Biochemistry 2010 11:37 doi:10.1186/1471-2091-11-37