Table 3

Inhibition constants of anionic inhibitors against various CAs

Inhibitor

K_I [mM]^#

HCA II

Nce103

ScCA

CgCA

DmBCA

F^-

>300

0.69

2.85

0.36

0.80

Cl^-

200

0.85

0.85

0.58

0.97

Br^-

63

0.94

0.0108

27

1.04

I^-

26

1.40

0.0103

42.4

1.18

CNO^-

0.03

1.18

31.7

0.60

0.73

SCN^-

1.6

0.65

55.6

0.73

1.28

CN^-

0.02

0.011

16.8

1.12

0.67

N_3^-

1.5

0.52

27.9

1.03

1.12

HCO_3^-

85

0.62

0.78

0.086

26.9

NO_3^-

35

0.69

13.9

0.097

43.7

NO_2^-

63

0.53

0.46

0.088

28.6

HS^-

0.04

0.37

0.33

0.10

1.01

HSO_3^-

89

0.54

0.33

0.10

1.29

SO_4^2-

>200

14.15

0.58

0.58

1.36

ClO_4^-

>200

>200

>200

>200

>200

H₂NSO₂NH₂

1.13

0.30

0.0087

0.42

0.15

H₂NSO₃H^§

0.39

0.70

0.84

0.11

2.45

Ph-B(OH)₂

23.1

30.8

38.2

0.10

22.4

Ph-AsO₃H_2^§

49.2

30.8

0.40

0.098

32.6

Inhibition constants against isozymes HCA II (α-CA class), and the β-CAs Nce103 (from C. albicans), ScCA (from S. cerevisiae), CgCA (from C. glabrata) and DmBCA (D. melanogaster) for the CO₂ hydration reaction at 20°C are shown.

^§As sodium salt.

^# Errors in the range of 5-10% of the shown data, from three different assays, by a CO2 hydration stopped-flow assay.

Syrjänen et al. BMC Biochemistry 2010 11:28   doi:10.1186/1471-2091-11-28