Research article
Loss of the SIN3 transcriptional corepressor results in aberrant mitochondrial function
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* Corresponding author: Lori A Pile loripile@wayne.edu
1 Department of Biological Sciences, Wayne State University, 5047 Gullen Mall, Detroit, Michigan 48202, USA
2 Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 540 E. Canfield, Detroit, Michigan 48201, USA
3 Chemical Biology, University of Michigan, 930 N. University Ave. Rm. 4250, Ann Arbor, Michigan 48109, USA
BMC Biochemistry 2010, 11:26 doi:10.1186/1471-2091-11-26
Published: 9 July 2010Additional files
Additional file 1:
Primer pairs used in this study. This table lists all primer pairs used for qPCR gene expression analysis.
Format: XLS Size: 24KB Download file
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Additional file 2:
sin3 mutant cells grow very poorly on solid agar plates containing non-fermentable carbon sources. This file contains images showing the growth of wild type and a sin3 mutant on solid agar plates containing YPD or non-fermentable carbon sources.
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Additional file 3:
Additional S. cerevisiae strains demonstrate ySin3 is critical for growth in media prepared with non-fermentable carbon sources. This file shows growth curves for two additional S. cerevisiae strains, with differing genetic backgrounds, in media prepared with YPD or non-fermentable carbon sources.
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Additional file 4:
A second S. cerevisiae strain demonstrates ySin3 is critical for growth on media prepared with non-fermentable carbon sources. This file contains images showing the growth of wild type and a sin3 mutant of different genetic background on solid agar plates containing YPD or non-fermentable carbon sources.
Format: PDF Size: 1.5MB Download file
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Additional file 5:
A third S. cerevisiae strain demonstrates ySin3 is critical for growth on media prepared with non-fermentable carbon sources. This file contains images showing the growth of wild type and a sin3 mutant of different genetic background on solid agar plates containing YPD or non-fermentable carbon sources.
Format: PDF Size: 1.6MB Download file
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Additional file 6:
Mitochondrial genomes are observed in all sin3 and rpd3 mutants. This file contains images of DAPI staining of nuclear and mitochondrial DNA in wild type and sin3 and rpd3 mutant cells.
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Additional file 7:
rpd3 mutant cells grow very poorly on solid agar plates containing non-fermentable carbon sources. This file contains images showing the growth of wild type and an rpd3 mutant on solid agar plates containing YPD or non-fermentable carbon sources.
Format: PDF Size: 1.7MB Download file
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