Table 2 |
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|
Pi-dependent activation of inactivated E. coli PEPS |
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|
Reaction mixtures |
PEPS activity at zero time (%) |
PEPS activity after 5 min (%) |
PEPS activity after 10 min (%) |
|
|
|||
|
Complete (plus 1 mM Pi) |
6 |
83 |
92 |
|
|
|||
|
Minus 1 mM Pi |
6 |
11 |
15 |
|
|
|||
|
Plus Pi and 2 mM ADP |
5 |
13 |
17 |
|
|
|||
|
Plus Pi and 2 mM AMP |
6 |
18 |
27 |
|
|
|||
|
The experiment was repeated five times; the results of one experiment, representative of all five experiments, are presented in the table. |
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|
Burnell BMC Biochemistry 2010 11:1 doi:10.1186/1471-2091-11-1 |
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