Expression of mutant BCMO1 constructs in transformed cell lines. A) Quantification of expression of F51 and W454 mutants. B) Quantification of expression of Y235 and Y326 mutants. The cell extracts were probed with a monoclonal anti-His antibody. The intensity of each mutant BCMO1 band was quantified by densitometry and normalized to intensity of recombinant BCMO1 (0.1 μg) synthesized by Roche RTS cell-free transcription/translation on each gel.
Poliakov et al. BMC Biochemistry 2009 10:31 doi:10.1186/1471-2091-10-31