Table 7 |
||
|
Reactivation of pCMB treated CPRHG by sulfhydryl reagents |
||
|
Substance added |
Concentration (mM) |
Relative activity (%) |
|
|
||
|
Control |
- |
100 |
|
pCMB |
10 |
0 |
|
pCMB + β-ME |
5 10 |
53 77 |
|
pCMB + DTT |
5 10 |
73 75 |
|
pCMB +Glutathione |
5 10 |
85 104 |
|
pCMB + Cysteine |
5 10 |
71 83 |
|
|
||
|
The hydrolyzing activity is expressed as per cent of control (19.73 units/h/mg enzyme) |
||
|
Jinka et al. BMC Biochemistry 2009 10:28 doi:10.1186/1471-2091-10-28 |
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