Figure 1.

Fractionation of enzyme on DEAE-Cellulose column: The enzyme fraction obtained from ammonium sulfate precipitation (30-60%) was loaded on to DEAE-Cellulose column equilibrated with equilibrating buffer (0.01 M Tris- HCl buffer, pH 7.0, containing 2 mM β-ME) at a flow rate of 40 ml/hr and 5 ml fractions were collected and assayed for enzyme activity as described in materials and methods. The indicated fractions (14-20) were pooled for further processing.