Trypsin fragmentation of GST-PP6R3 and CD spectrum of SAPS domain. (A) Recombinant GST-PP6R3 (upper band, open arrow) was digested with trypsin from 0 to 10 min. The GST-PP6R3 and fragments were detected using an anti-GST antibody and a non-specific band (labelled C) that was not digested served as loading control Trypsin caused time-dependent formation of four digestion products (arrows). GST was digested under identical conditions for 10 min (left 2 lanes). The migration of molecular size standards is indicated to the left side of the frame. (B) Circular dichroic (CD) spectrum of purified recombinant PP6R3(1-513) plotted as molar ellipticity (× 10-5) vs. wavelength in nm. (C) Recombinant His6 tagged SAPS domain residues 1-513, purified and stained with Coomassie after SDS-PAGE. Left lane, size standards in kDa; right lane is protein used for CD spectrum.
Guergnon et al. BMC Biochemistry 2009 10:24 doi:10.1186/1471-2091-10-24