Figure 5.

Determination of the kinetic parameters of malate synthase G of P. aeruginosa. The formulas for the calculation of the kinetic parameters are indicated. In the formulas, subscript A and B refer to glyoxylate and acetyl CoA, respectively. (A) The primary Lineweaver-Burk plots show reciprocal velocity (minutes/nmol) in function of the reciprocal glyoxylate concentration (in μM^-1). Each line represents a concentration of acetyl CoA: 200 (black diamond), 100 (black square), 50 (black triangle), 25 (black X), 15 μM (black dot). R² of the trendlines are 0.995, 0.999, 0.989, 0.996 and 0.982, respectively. (B) The secondary plot gives the reciprocal apparent velocities (in minutes/nmol) as a function of the reciprocal acetyl CoA concentration (in μM^-1). The R² of the trendline is 0.9781.