Table 2 |
||||
|
Single stranded DNA substrates used |
||||
|
Substrate |
Source |
Primers |
Substrate size (bases) |
%GC |
|
|
||||
|
PBAD |
pBAD/thio |
Left-ATGCCATAGCATTTTTATCG Right-*GACCGGTACGCGTAGAATCG |
556 |
47 |
|
MdnaK |
M. tuberculosis dnaK |
Left-*GAACCCGTTGTTCTTAGACGAG Right-*GGGTAACATCAAGCAGCAGAAC |
317 |
62 |
|
Mtop |
M. tuberculosis topA |
Left-*GTAGAAGTTGTTGAGCCAGT Right-TACTCGTCGATCATCAAGAC |
216 |
61 |
|
|
||||
|
* Primer 32P labeled at 5'end to analyze extended strand as cleavage substrate after denaturation of the PCR product. The left and right primers of MdnaK were utilized in separate experiments. |
||||
|
Annamalai et al. BMC Biochemistry 2009 10:18 doi:10.1186/1471-2091-10-18 |
||||
