Table 2 |
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|
DNA binding kinetics and affinities of f-PlnC and f-PlnD. |
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|
Analyte |
Ligand |
Ka1 (M-1s-1) |
Kd1 (s-1) |
Ka2 (M-1s-1) |
Kd2 (s-1) |
aKD1 (M) |
KD2 (M) |
KD1/KD2 |
|
|
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|
f-PlnC |
PplnA |
6.8 ± 6.1 × 102 |
1.0 ± 0.1 × 10-3 |
1.5 × 105 |
1.0 × 10-3 |
1.5 ± 5.3 × 10-6 |
6.7 ± 0.1 × 10-9 |
2.2 × 102 |
|
PplnM |
3.9 ± 0.9 × 103 |
1.5 ± 0.1 × 10-3 |
2.3 ± 0.1 × 105 |
7.5 ± 0.5 × 10-4 |
3.8 ± 0.6 × 10-7 |
3.2 ± 0.3 × 10-9 |
1.2 × 102 |
|
|
PplnJ |
NA |
NA |
NA |
NA |
NA |
NA |
||
|
PplnE |
1.2 ± 0.9 × 104 |
2.5 ± 0.1 × 10-3 |
2.4 ± 0.1 × 105 |
2.6 ± 0.5 × 10-4 |
2.1 ± 0.2 × 10-7 |
1.1 ± 0.3 × 10-9 |
1.9 × 102 |
|
|
bPplnG |
8.7 ± 7.1 × 103 |
1.5 ± 0.4 × 10-3 |
1.3 ± 0.6 × 104 |
1.3 ± 0.1 × 10-3 |
1.7 ± 3.9 × 10-7 |
1.0 ± 0.5 × 10-7 |
1.7 |
|
|
PplnAΔL |
3.2 ± 1.2 × 104 |
9.6 ± 0.1 × 10-3 |
3.0 ± 1.3 × 10-7 |
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|
f-PlnD |
PplnA |
3.4 × 103 |
5.0 × 10-3 |
3.4 ± 0.1 × 103 |
5.1 ± × 10-3 |
1.5 × 10-6 |
1.5 ± 0.1 × 10-6 |
1.0 |
|
PplnM |
3.9 × 103 |
2.2 × 10-3 |
1.4 × 104 |
1.1 ± 0.3 × 10-6 |
5.6 × 10-7 |
7.8 ± 0.3 × 10-11 |
7.2 × 103 |
|
|
PplnJ |
9.5 ± 0.3 × 102 |
1.8 ± 0.1 × 10-2 |
2.3 ± 0.1 × 102 |
2.2 ± 0.3 × 10-5 |
1.2 ± 0.4 × 10-5 |
9.5 ± 1.4 × 10-8 |
1.3 × 102 |
|
|
PplnE |
NA |
NA |
NA |
NA |
NA |
NA |
||
|
PplnG |
1.1 ± 0.1 × 103 |
1.5 ± 0.1 × 10-2 |
2.5 ± 0.1 × 102 |
9.3 ± 8.7 × 10-6 |
1.4 ± 1.1 × 10-5 |
3.7 ± 2.3 × 10-8 |
3.8 × 102 |
|
|
PplnAΔL |
NA |
NA |
NA |
NA |
NA |
NA |
||
|
|
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|
a KD1 = kd1/ka1, KD2 = kd2/ka2 b The ka was fitted locally NA: not available |
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|
Straume et al. BMC Biochemistry 2009 10:17 doi:10.1186/1471-2091-10-17 |
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