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51.

2832
Accesses

Commentary   Open Access Highly Accessed

The Hospital Anxiety And Depression Scale

R Philip Snaith Health and Quality of Life Outcomes 2003, 1:29 (1 August 2003)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

52.

2773
Accesses

Research article   Open Access Highly Accessed

A comparison of methods for differential expression analysis of RNA-seq data

Charlotte Soneson, Mauro Delorenzi BMC Bioinformatics 2013, 14:91 (9 March 2013)

Abstract | Full text | PDF | ePUB | PubMed

53.

2765
Accesses

Method   Open Access Highly Accessed

Differential expression analysis for sequence count data

Simon Anders, Wolfgang Huber Genome Biology 2010, 11:R106 (27 October 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment | F1000 Biology |  Editor’s summary

DEseq allows the determination of differential expression of read count data from RNA-seq or ChIP-seq experiments

54.

2759
Accesses

Review   Open Access Highly Accessed

Nutritional therapies for mental disorders

Shaheen E Lakhan, Karen F Vieira Nutrition Journal 2008, 7:2 (21 January 2008)

Abstract | Full text | PDF | PubMed | 1 comment

55.

2748
Accesses

Research article   Open Access Highly Accessed

Amniotic fluid embolism incidence, risk factors and outcomes: a review and recommendations

Marian Knight, Cynthia Berg, Peter Brocklehurst, Michael Kramer, Gwyneth Lewis, Jeremy Oats, Christine L Roberts, Catherine Spong, Elizabeth Sullivan, Jos van Roosmalen, Joost Zwart BMC Pregnancy and Childbirth 2012, 12:7 (10 February 2012)

Abstract | Full text | PDF | PubMed

56.

2737
Accesses

Research article   Open Access Highly Accessed

Depression, antidepressant medications, and risk of Clostridium difficile infection

Mary A M Rogers, M Todd Greene, Vincent B Young, Sanjay Saint, Kenneth M Langa, John Y Kao, David M Aronoff BMC Medicine 2013, 11:121 (7 May 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

The risk of Clostridium difficile infection is increased in adults with depression and in those taking antidepressants during hospitalization, indicating that physicians should be aware of the elevated infection risk in these patients.

57.

2721
Accesses

Software   Open Access Highly Accessed

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

Ben Langmead, Cole Trapnell, Mihai Pop, Steven L Salzberg Genome Biology 2009, 10:R25 (4 March 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Bowtie: a new ultrafast memory-efficient tool for the alignment of short DNA sequence reads to large genomes.

58.

2705
Accesses

Research article   Open Access Highly Accessed

Relationship between Organizational Culture, Leadership Behavior and Job Satisfaction

Yafang Tsai BMC Health Services Research 2011, 11:98 (14 May 2011)

Abstract | Full text | PDF | PubMed

59.

2705
Accesses

Commentary   Open Access Highly Accessed

Processing of meats and cardiovascular risk: time to focus on preservatives

Renata Micha, Georgios Michas, Martin Lajous, Dariush Mozaffarian BMC Medicine 2013, 11:136 (23 May 2013)

Abstract | Full text | PDF | PubMed | 1 comment |  Editor’s summary

Dariush Mozaffarian and colleagues comment on a study showing that processed meat is linked to increased mortality risk, and emphasize how health guidance should recommend avoiding processed meat rather than focusing on fat content.

60.

2693
Accesses

Commentary   Open Access Highly Accessed

International Society of Sports Nutrition position stand: creatine supplementation and exercise

Thomas W Buford, Richard B Kreider, Jeffrey R Stout, Mike Greenwood, Bill Campbell, Marie Spano, Tim Ziegenfuss, Hector Lopez, Jamie Landis, Jose Antonio Journal of the International Society of Sports Nutrition 2007, 4:6 (30 August 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

61.

2693
Accesses

Research   Open Access Highly Accessed

Hookah smoking and cancer: carcinoembryonic antigen (CEA) levels in exclusive/ever hookah smokers

Khan Sajid, Kamal Chaouachi, Rubaida Mahmood Harm Reduction Journal 2008, 5:19 (24 May 2008)

Abstract | Full text | PDF | PubMed | 8 comments

62.

2671
Accesses

Review   Open Access Highly Accessed

Intestinal microbiota, probiotics and mental health: from Metchnikoff to modern advances: Part I – autointoxication revisited

Alison C Bested, Alan C Logan, Eva M Selhub Gut Pathogens 2013, 5:5 (18 March 2013)

Abstract | Full text | PDF | ePUB | PubMed

63.

2605
Accesses

Research   Open Access Highly Accessed

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman Genome Biology 2002, 3:research0034-research0034.11 (18 June 2002)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment |  Editor’s summary

Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested.

64.

2559
Accesses

Research article   Open Access Highly Accessed

Love thy neighbour: facilitation through an alternative signalling modality in plants

Monica Gagliano, Michael Renton BMC Ecology 2013, 13:19 (7 May 2013)

Abstract | Full text | PDF | ePUB | PubMed |  Editor’s summary

Chilli seedlings germinate better when grown in the presence of ‘good neighbour’ plants like basil even when all forms of chemical and visual signals are blocked, suggesting that an as-yet unidentified system of communication exists between plants.

65.

2519
Accesses

Research   Open Access Highly Accessed

Virophages, polintons, and transpovirons: a complex evolutionary network of diverse selfish genetic elements with different reproduction strategies

Natalya Yutin, Didier Raoult, Eugene V Koonin Virology Journal 2013, 10:158 (23 May 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

A phylogenomic analysis found that giant viruses, virophages and transposable elements have a complex evolutionary relationship, suggesting that viruses evolved from non-viral mobile genetic elements and vice versa, on more than one occasion.

66.

2509
Accesses

Research   Open Access Highly Accessed

Policymakers’ and other stakeholders’ perceptions of key considerations for health system decisions and the presentation of evidence to inform those considerations: an international survey

Joshua P Vogel, Andrew D Oxman, Claire Glenton, Sarah Rosenbaum, Simon Lewin, A Gülmezoglu, João Souza Health Research Policy and Systems 2013, 11:19 (24 May 2013)

Abstract | Full text | PDF | PubMed

67.

2502
Accesses

Brief Report   Open Access Highly Accessed

Diminished rostral anterior cingulate cortex activation during trauma-unrelated emotional interference in PTSD

Reid Offringa, Kathryn Handwerger Brohawn, Lindsay K Staples, Stacey J Dubois, Katherine C Hughes, Danielle L Pfaff, Michael B VanElzakker, F Davis, Lisa M Shin Biology of Mood & Anxiety Disorders 2013, 3:10 (14 May 2013)

Abstract | Full text | PDF | ePUB | PubMed

68.

2495
Accesses

Research   Open Access Highly Accessed

Mutations within lncRNAs are effectively selected against in fruitfly but not in human

Wilfried Haerty, Chris P Ponting Genome Biology 2013, 14:R49 (27 May 2013)

Abstract | Provisional PDF | PubMed |  Editor’s summary

Analysis of lncRNA conservation in fruitfly and humans shows that these elements are well conserved in insects, but not in mammals

69.

2494
Accesses

Research   Open Access Highly Accessed

DNA methylation dynamics during intestinal stem cell differentiation reveals enhancers driving gene expression in the villus

Lucas JT Kaaij, Marc van de Wetering, Fang Fang, Benjamin Decato, Antoine Molaro, Harmen JG van de Werken, Johan H van Es, Jurian Schuijers, Elzo de Wit, Wouter de Laat, Gregory J Hannon, Hans C Clevers, Andrew D Smith, Rene F Ketting Genome Biology 2013, 14:R50 (28 May 2013)

Abstract | Provisional PDF | PubMed |  Editor’s summary

Intriguingly, DNA methylation in mouse intestinal cells does not change much during differentiation

70.

2492
Accesses

Method   Open Access Highly Accessed

Integrated analysis of recurrent properties of cancer genes to identify novel drivers

Matteo D'Antonio, Francesca D Ciccarelli Genome Biology 2013, 14:R52 (29 May 2013)

Abstract | Provisional PDF | PubMed |  Editor’s summary

A method to detect rare driver genes through integrated analysis of expression profiles, mutation effects and properties of known cancer genes

71.

2471
Accesses

Review   Open Access Highly Accessed

Protein timing and its effects on muscular hypertrophy and strength in individuals engaged in weight-training

Matthew Stark, Judith Lukaszuk, Aimee Prawitz, Amanda Salacinski Journal of the International Society of Sports Nutrition 2012, 9:54 (14 December 2012)

Abstract | Full text | PDF | PubMed

72.

2461
Accesses

Research article   Open Access Highly Accessed

The weight of nations: an estimation of adult human biomass

Sarah Walpole, David Prieto-Merino, Phil Edwards, John Cleland, Gretchen Stevens, Ian Roberts BMC Public Health 2012, 12:439 (18 June 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

National food energy requirements are primarily determined by the total biomass of the population rather than the population, suggesting that increasing body mass could have the same implications for world energy requirements as an extra 473 million people on earth.

73.

2444
Accesses

Research   Open Access Highly Accessed

A survey of energy drink consumption patterns among college students

Brenda M Malinauskas, Victor G Aeby, Reginald F Overton, Tracy Carpenter-Aeby, Kimberly Barber-Heidal Nutrition Journal 2007, 6:35 (31 October 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

74.

2441
Accesses

Research article   Open Access Highly Accessed

A phylogeny and revised classification of Squamata, including 4161 species of lizards and snakes

R Pyron, Frank T Burbrink, John J Wiens BMC Evolutionary Biology 2013, 13:93 (29 April 2013)

Abstract | Full text | PDF | PubMed

75.

2442
Accesses

Review   Open Access Highly Accessed

Toxic epidermal necrolysis and Stevens-Johnson syndrome

Thomas Harr, Lars E French Orphanet Journal of Rare Diseases 2010, 5:39 (16 December 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

76.

2441
Accesses

Research   Open Access Highly Accessed

The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide

Monica H Carlsen, Bente L Halvorsen, Kari Holte, Siv K Bøhn, Steinar Dragland, Laura Sampson, Carol Willey, Haruki Senoo, Yuko Umezono, Chiho Sanada, Ingrid Barikmo, Nega Berhe, Walter C Willett, Katherine M Phillips, David R Jacobs, Rune Blomhoff Nutrition Journal 2010, 9:3 (22 January 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

77.

2439
Accesses

Review   Open Access Highly Accessed

Applications of nanoparticles in biology and medicine

OV Salata Journal of Nanobiotechnology 2004, 2:3 (30 April 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

78.

2430
Accesses

Letter   Free Highly Accessed

Target blood pressure in sepsis: between a rock and a hard place

Thiago Corrêa, Jukka Takala, Stephan Jakob Critical Care 2013, 17:433 (17 May 2013)

Abstract | Full text | PDF | PubMed

79.

2429
Accesses

Research article   Open Access Highly Accessed

Clinical outcomes resulting from telemedicine interventions: a systematic review

William R Hersh, Mark Helfand, James Wallace, Dale Kraemer, Patricia Patterson, Susan Shapiro, Merwyn Greenlick BMC Medical Informatics and Decision Making 2001, 1:5 (26 November 2001)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Randomized controlled trials are needed, in all areas of health care, to determine the efficacy of telemedicine for clinical outcomes.

80.

2423
Accesses

Research   Open Access Highly Accessed

No connection between the level of exposition to statins in the population and the incidence/mortality of acute myocardial infarction: An ecological study based on Sweden's municipalities

Staffan Nilsson, Sigvard Mölstad, Catarina Karlberg, Jan-Erik Karlsson, Lars-Göran Persson Journal of Negative Results in BioMedicine 2011, 10:6 (24 May 2011)

Abstract | Full text | PDF | PubMed

81.

2421
Accesses

Review   Open Access Highly Accessed

Beta-thalassemia

Renzo Galanello, Raffaella Origa Orphanet Journal of Rare Diseases 2010, 5:11 (21 May 2010)

Abstract | Full text | PDF | PubMed

82.

2404
Accesses

Meeting report   Subscription

Epigenetics and transgenerational inheritance

Emilie Brasset, Séverine Chambeyron Genome Biology 2013, 14:306 (24 May 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

A report on the 'Non-coding RNA, epigenetics and transgenerational inheritance' meeting, held at Churchill College, Cambridge, UK, April 11-12, 2013.

83.

2366
Accesses

Research   Open Access Highly Accessed

Acute renal failure – definition, outcome measures, animal models, fluid therapy and information technology needs: the Second International Consensus Conference of the Acute Dialysis Quality Initiative (ADQI) Group

Rinaldo Bellomo, Claudio Ronco, John A Kellum, Ravindra L Mehta, Paul Palevsky, the ADQI workgroup Critical Care 2004, 8:R204-R212 (24 May 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment | F1000

84.

2365
Accesses

Research   Open Access Highly Accessed

Characterizing the admixed African ancestry of African Americans

Fouad Zakharia, Analabha Basu, Devin Absher, Themistocles L Assimes, Alan S Go, Mark A Hlatky, Carlos Iribarren, Joshua W Knowles, Jun Li, Balasubramanian Narasimhan, Steven Sidney, Audrey Southwick, Richard M Myers, Thomas Quertermous, Neil Risch, Hua Tang Genome Biology 2009, 10:R141 (22 December 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Genome-wide SNP analyses reveal the admixed African genetic ancestry of African Americans.

85.

2356
Accesses

Methodology   Open Access Highly Accessed

Therapeutic emails

Farrokh Alemi, Mary R Haack, Susanna Nemes, Renita Aughburns, Jennifer Sinkule, Duncan Neuhauser Substance Abuse Treatment, Prevention, and Policy 2007, 2:7 (16 February 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

86.

2356
Accesses

Research   Open Access Highly Accessed

The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage

H Bradley Shaffer, Patrick Minx, Daniel E Warren, Andrew M Shedlock, Robert C Thomson, Nicole Valenzuela, John Abramyan, Chris T Amemiya, Daleen Badenhorst, Kyle K Biggar, Glen M Borchert, Christopher W Botka, Rachel M Bowden, Edward L Braun, Anne M Bronikowski, Benoit G Bruneau, Leslie T Buck, Blanche Capel, Todd A Castoe, Mike Czerwinski, Kim D Delehaunty, Scott V Edwards, Catrina C Fronick, Matthew K Fujita, Lucinda Fulton, Tina A Graves, Richard E Green, Wilfried Haerty, Ramkumar Hariharan, Omar Hernandez et al. Genome Biology 2013, 14:R28 (28 March 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

The genome of the western painted turtle, Chrysemys picta bellii, reveals insights into its extreme tolerance to anoxia and freezing

87.

2310
Accesses

Research article   Open Access Highly Accessed

Women with symptoms of uncomplicated urinary tract infection are often willing to delay antibiotic treatment: a prospective cohort study

Bart J Knottnerus, Suzanne E Geerlings, Eric P van Charante, Gerben ter Riet BMC Family Practice 2013, 14:71 (31 May 2013)

Abstract | Full text | PDF | ePUB | PubMed |  Editor’s summary

Over a third of women presenting with urinary tract infection symptoms are happy to delay antibiotic treatment when asked by their GP; the majority of these patients show an improvement in symptoms without further treatment

88.

2306
Accesses

Research article   Open Access

Benchmarking short sequence mapping tools

Ayat Hatem, Doruk Bozda¿, Amanda E Toland, Ümit V Çatalyürek BMC Bioinformatics 2013, 14:184 (7 June 2013)

Abstract | Provisional PDF | PubMed |  Editor’s summary

A benchmarking suite to analyze well-known next generation sequencing tools, mapping short reads to a reference genome, with respect to various technological and algorithmic perspectives and addressing issues using multiple approaches such as multiple simulators.

89.

2299
Accesses

Opinion   Open Access

Thirty years on: HIV receptor gymnastics and the prevention of infection

Robin A Weiss BMC Biology 2013, 11:57 (21 May 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Robin Weiss, in an article to mark the 30th anniversary of the discovery of the human immunodeficiency virus (HIV), reviews the complex interactions of the virus with the specialized surface molecules by which it gains entry into cells, and how this has led to drugs that block it.

90.

2284
Accesses

Review   Open Access Highly Accessed

The Bhopal disaster and its aftermath: a review

Edward Broughton Environmental Health: A Global Access Science Source 2005, 4:6 (10 May 2005)

Abstract | Full text | PDF | PubMed

91.

2273
Accesses

Commentary   Open Access

Body mass index and musculoskeletal pain: is there a connection?

David R Seaman Chiropractic & Manual Therapies 2013, 21:15 (20 May 2013)

Abstract | Full text | PDF | ePUB | PubMed |  Editor’s summary

http://commons.wikimedia.org/wiki/File%3AOverweight_waistline.jpg

92.

2258
Accesses

Review   Open Access Highly Accessed

Nutrition and cancer: A review of the evidence for an anti-cancer diet

Michael S Donaldson Nutrition Journal 2004, 3:19 (20 October 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment

93.

2255
Accesses

Review   Open Access Highly Accessed

Venous hemodynamics in neurological disorders: an analytical review with hydrodynamic analysis

Clive B Beggs BMC Medicine 2013, 11:142 (31 May 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Neurological disorders, such as multiple sclerosis (MS) and leukoaraiosis have been associated with venous abnormalities that contribute to the pathophysiology; Clive Beggs reviews the evidence for this, concluding that the role of cerebral flow in MS remains unclear.

94.

2256
Accesses

Commentary   Open Access Highly Accessed

Why Do We Put Cervical Collars On Conscious Trauma Patients?

Jonathan Benger, Julian Blackham Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine 2009, 17:44 (18 September 2009)

Abstract | Full text | PDF | PubMed

95.

2249
Accesses

Study protocol   Open Access

Implementing the ten steps to successful breastfeeding in multiple hospitals serving low-wealth patients in the US: innovative research design and baseline findings

Miriam H Labbok, Emily C Taylor, Nathan C Nickel International Breastfeeding Journal 2013, 8:5 (20 May 2013)

Abstract | Full text | PDF | ePUB | PubMed

96.

2232
Accesses

Review   Open Access Highly Accessed

International Society of Sports Nutrition position stand: energy drinks

Bill Campbell, Colin Wilborn, Paul La Bounty, Lem Taylor, Mike T Nelson, Mike Greenwood, Tim N Ziegenfuss, Hector L Lopez, Jay R Hoffman, Jeffrey R Stout, Stephen Schmitz, Rick Collins, Doug S Kalman, Jose Antonio, Richard B Kreider Journal of the International Society of Sports Nutrition 2013, 10:1 (3 January 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

A systematic review on energy drinks, from the International Society of Sports Nutrition.

97.

2197
Accesses

Research article   Open Access Highly Accessed

Ubiquinol supplementation enhances peak power production in trained athletes: a double-blind, placebo controlled study

Dietmar Alf, Michael E Schmidt, Stefan C Siebrecht Journal of the International Society of Sports Nutrition 2013, 10:24 (29 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

98.

2230
Accesses

Method   Open Access Highly Accessed

Quartz-Seq: a highly reproducible and sensitive single-cell RNA-Seq reveals non-genetic gene expression heterogeneity

Yohei Sasagawa, Itoshi Nikaido, Tetsutaro Hayashi, Hiroki Danno, Kenichiro D Uno, Takeshi Imai, Hiroki R Ueda Genome Biology 2013, 14:R31 (17 April 2013)

Abstract | Provisional PDF | PubMed |  Editor’s summary

Quartz-Seq is a method for performing single cell RNA-seq which displays high reproducibility

99.

2231
Accesses

Research   Open Access

Insights into snoRNA biogenesis and processing from PAR-CLIP of snoRNA core proteins and small RNA sequencing

Shivendra Kishore, Andreas R Gruber, Dominik J Jedlinski, Afzal P Syed, Hadi Jorjani, Mihaela Zavolan Genome Biology 2013, 14:R45 (26 May 2013)

Abstract | Provisional PDF | PubMed |  Editor’s summary

PAR-CLIP of snoRNA-associated proteins reveals the existence of extensive human loci that are processed into snoRNAs

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