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Yeast biotechnology

Edited by Prof Diethard Mattanovich

Microbial Cell Factories invites submissions to the recently launched thematic series on “Yeast biotechnology”.

Mankind has exploited the metabolic activities of yeasts in baking and brewing for several millennia. The invention of the fed-batch technology for production of bakers' yeast has been a cornerstone of modern biotechnology, enabling the development of efficient production processes for antibiotics, biopharmaceuticals and technical enzymes. Alcoholic fermentation, on the other hand, is a model for microbial metabolite production and has become a major research focus for the manufacturing of biofuels. Expanding from our knowledge on bakers' yeast, diverse yeast species are explored today. The series highlights the latest research to produce biopharmaceuticals, enzymes, alcohols, organic acids, flavours and fine chemicals, underlining the huge potential of yeast biotechnology.

This collection of articles has not been sponsored and articles have undergone the journal’s standard peer-review process. The Guest Editors declare no competing interests.

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  1. Research

    The effect of hypoxia on the lipidome of recombinant Pichia pastoris

    Cultivation of recombinant Pichia pastoris (Komagataella sp.) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expres...

    Núria Adelantado, Pablo Tarazona, Karlheinz Grillitsch, Xavier García-Ortega, Sergi Monforte, Francisco Valero, Ivo Feussner, Günther Daum and Pau Ferrer

    Microbial Cell Factories 2017 16:86

    Published on: 19 May 2017

  2. Research

    Functional inclusion bodies produced in the yeast Pichia pastoris

    Bacterial inclusion bodies (IBs) are non-toxic protein aggregates commonly produced in recombinant bacteria. They are formed by a mixture of highly stable amyloid-like fibrils and releasable protein species wi...

    Fabián Rueda, Brigitte Gasser, Alejandro Sánchez-Chardi, Mònica Roldán, Sandra Villegas, Verena Puxbaum, Neus Ferrer-Miralles, Ugutz Unzueta, Esther Vázquez, Elena Garcia-Fruitós, Diethard Mattanovich and Antonio Villaverde

    Microbial Cell Factories 2016 15:166

    Published on: 1 October 2016

  3. Research

    Engineering Saccharomyces cerevisiae with the deletion of endogenous glucosidases for the production of flavonoid glucosides

    Glycosylation of flavonoids is a promising approach to improve the pharmacokinetic properties and biological activities of flavonoids. Recently, many efforts such as enzymatic biocatalysis and the engineered Esch...

    Huimin Wang, Yan Yang, Lin Lin, Wenlong Zhou, Minzhi Liu, Kedi Cheng and Wei Wang

    Microbial Cell Factories 2016 15:134

    Published on: 4 August 2016

  4. Research

    Characterisation of the broad substrate specificity 2-keto acid decarboxylase Aro10p of Saccharomyces kudriavzevii and its implication in aroma development

    The yeast amino acid catabolism plays an important role in flavour generation since higher alcohols and acetate esters, amino acid catabolism end products, are key components of overall flavour and aroma in fe...

    Jiri Stribny, Gabriele Romagnoli, Roberto Pérez-Torrado, Jean-Marc Daran and Amparo Querol

    Microbial Cell Factories 2016 15:51

    Published on: 12 March 2016

  5. Research

    Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion

    Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combine...

    Danna Lee, Natoiya D. R. Lloyd, Isak S. Pretorius and Anthony R. Borneman

    Microbial Cell Factories 2016 15:49

    Published on: 4 March 2016

  6. Research

    ATP citrate lyase mediated cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae

    With increasing concern about the environmental impact of a petroleum based economy, focus has shifted towards greener production strategies including metabolic engineering of microbes for the conversion of pl...

    Sarah Rodriguez, Charles M. Denby, T. Van Vu, Edward E. K. Baidoo, George Wang and Jay D. Keasling

    Microbial Cell Factories 2016 15:48

    Published on: 3 March 2016

  7. Research

    Production of the forskolin precursor 11β-hydroxy-manoyl oxide in yeast using surrogate enzymatic activities

    Several plant diterpenes have important biological properties. Among them, forskolin is a complex labdane-type diterpene whose biological activity stems from its ability to activate adenylyl cyclase and to ele...

    Codruta Ignea, Efstathia Ioannou, Panagiota Georgantea, Fotini A. Trikka, Anastasia Athanasakoglou, Sofia Loupassaki, Vassilios Roussis, Antonios M. Makris and Sotirios C. Kampranis

    Microbial Cell Factories 2016 15:46

    Published on: 26 February 2016

  8. Research

    Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

    Lactic acid is a versatile chemical platform with many different industrial applications. Yeasts have been demonstrated as attractive alternative to natural lactic acid producers since they can grow at low pH,...

    Nadia Maria Berterame, Danilo Porro, Diletta Ami and Paola Branduardi

    Microbial Cell Factories 2016 15:39

    Published on: 17 February 2016

  9. Research

    Increased availability of NADH in metabolically engineered baker’s yeast improves transaminase-oxidoreductase coupled asymmetric whole-cell bioconversion

    Saccharomyces cerevisiae can be engineered to perform a multitude of different chemical reactions that are not programmed in its original genetic code. It has a large potential to func...

    Jan Dines Knudsen, Cecilia Hägglöf, Nora Weber and Magnus Carlquist

    Microbial Cell Factories 2016 15:37

    Published on: 15 February 2016

  10. Research

    Key role of lipid management in nitrogen and aroma metabolism in an evolved wine yeast strain

    Fermentative aromas play a key role in the organoleptic profile of young wines. Their production depends both on yeast strain and fermentation conditions. A present-day trend in the wine industry consists in d...

    Stéphanie Rollero, Jean-Roch Mouret, Isabelle Sanchez, Carole Camarasa, Anne Ortiz-Julien, Jean-Marie Sablayrolles and Sylvie Dequin

    Microbial Cell Factories 2016 15:32

    Published on: 9 February 2016

  11. Research

    Mechanism of imidazolium ionic liquids toxicity in Saccharomyces cerevisiae and rational engineering of a tolerant, xylose-fermenting strain

    Imidazolium ionic liquids (IILs) underpin promising technologies that generate fermentable sugars from lignocellulose for future biorefineries. However, residual IILs ar...

    Quinn Dickinson, Scott Bottoms, Li Hinchman, Sean McIlwain, Sheena Li, Chad L. Myers, Charles Boone, Joshua J. Coon, Alexander Hebert, Trey K. Sato, Robert Landick and Jeff S. Piotrowski

    Microbial Cell Factories 2016 15:17

    Published on: 20 January 2016

  12. Research

    Catabolism of coniferyl aldehyde, ferulic acid and p-coumaric acid by Saccharomyces cerevisiae yields less toxic products

    Lignocellulosic substrates and pulping process streams are of increasing relevance to biorefineries for second generation biofuels and biochemical production. They are known to be rich in sugars and inhibitors...

    Peter Temitope Adeboye, Maurizio Bettiga, Fredrik Aldaeus, Per Tomas Larsson and Lisbeth Olsson

    Microbial Cell Factories 2015 14:149

    Published on: 21 September 2015

  13. Research

    Three-pathway combination for glutathione biosynthesis in Saccharomyces cerevisiae

    Glutathione (GSH), a pivotal non-protein thiol, can be biosynthesized through three pathways in different organisms: (1) two consecutive enzymatic reactions catalyzed by γ-glutamylcysteine synthetase (Gsh1 or ...

    Liang Tang, Weiwei Wang, Wenlong Zhou, Kai Cheng, Yan Yang, Minzhi Liu, Kedi Cheng and Wei Wang

    Microbial Cell Factories 2015 14:139

    Published on: 16 September 2015

  14. Research

    Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain

    A Saccharomyces cerevisiae strain carrying deletions in all three pyruvate decarboxylase (PDC) genes (also called Pdc negative yeast) represents a non-ethanol producing platform strain for the production of pyruv...

    Yiming Zhang, Guodong Liu, Martin K M Engqvist, Anastasia Krivoruchko, Björn M Hallström, Yun Chen, Verena Siewers and Jens Nielsen

    Microbial Cell Factories 2015 14:116

    Published on: 8 August 2015

  15. Research

    An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures

    Membrane proteins are important drug targets in many human diseases and gathering structural information regarding these proteins encourages the pharmaceutical industry ...

    Augusto Q Pedro, Luís M Martins, João M L Dias, Maria J Bonifácio, João A Queiroz and Luís A Passarinha

    Microbial Cell Factories 2015 14:113

    Published on: 7 August 2015

  16. Research

    A novel process-based model of microbial growth: self-inhibition in Saccharomyces cerevisiae aerobic fed-batch cultures

    Microbial population dynamics in bioreactors depend on both nutrients availability and changes in the growth environment. Research is still ongoing on the optimization o...

    Stefano Mazzoleni, Carmine Landi, Fabrizio Cartenì, Elisabetta de Alteriis, Francesco Giannino, Lucia Paciello and Palma Parascandola

    Microbial Cell Factories 2015 14:109

    Published on: 30 July 2015

  17. Research

    Redox engineering by ectopic expression of glutamate dehydrogenase genes links NADPH availability and NADH oxidation with cold growth in Saccharomyces cerevisiae

    Cold stress reduces microbial growth and metabolism being relevant in industrial processes like wine making and brewing. Knowledge on the cold transcriptional response of S...

    Lidia Ballester-Tomás, Francisca Randez-Gil, Roberto Pérez-Torrado and Jose Antonio Prieto

    Microbial Cell Factories 2015 14:100

    Published on: 9 July 2015

  18. Research

    CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae

    One of the bottlenecks in production of biochemicals and pharmaceuticals in Saccharomyces cerevisiae is stable and homogeneous expression of pathway genes. Integration of genes into the genome of the production o...

    Carlotta Ronda, Jérôme Maury, Tadas Jakočiu̅nas, Simo Abdessamad Baallal Jacobsen, Susanne Manuela Germann, Scott James Harrison, Irina Borodina, Jay D Keasling, Michael Krogh Jensen and Alex Toftgaard Nielsen

    Microbial Cell Factories 2015 14:97

    Published on: 7 July 2015

  19. Review

    Yeast as a cell factory: current state and perspectives

    The yeast Saccharomyces cerevisiae is one of the oldest and most frequently used microorganisms in biotechnology with successful applications in the production of both bulk and fine chemicals. Yet, yeast research...

    Martin Kavšček, Martin Stražar, Tomaž Curk, Klaus Natter and Uroš Petrovič

    Microbial Cell Factories 2015 14:94

    Published on: 30 June 2015

  20. Technical Notes

    Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities

    Predictable control of gene expression is necessary for the rational design and optimization of cell factories. In the yeast Saccharomyces cerevisiae, the promoter is one of the most ...

    Bingyin Peng, Thomas C Williams, Matthew Henry, Lars K Nielsen and Claudia E Vickers

    Microbial Cell Factories 2015 14:91

    Published on: 26 June 2015

  21. Research

    Overexpression of a bifunctional enzyme, CrtS, enhances astaxanthin synthesis through two pathways in Phaffia rhodozyma

    A moderate-temperature, astaxanthin-overproducing mutant strain (termed MK19) of Phaffia rhodozyma was generated in our laboratory. The intracellular astaxanthin content of MK19 was 17-fold higher than that of wi...

    Shuang Chi, Yanfeng He, Jie Ren, Qian Su, Xingchao Liu, Zhi Chen, Mingan Wang, Ying Li and Jilun Li

    Microbial Cell Factories 2015 14:90

    Published on: 18 June 2015

  22. Research

    Production of β-ionone by combined expression of carotenogenic and plant CCD1 genes in Saccharomyces cerevisiae

    Apocarotenoids, like the C13-norisoprenoids, are natural compounds that contribute to the flavor and/or aroma of flowers and foods. They are produced in aromatic plants—like raspberries and roses—by the enzyma...

    Javiera López, Karen Essus, Il-kwon Kim, Rui Pereira, Jan Herzog, Verena Siewers, Jens Nielsen and Eduardo Agosin

    Microbial Cell Factories 2015 14:84

    Published on: 12 June 2015

  23. Research

    Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics

    L-tyrosine is a common precursor for a wide range of valuable secondary metabolites, including benzylisoquinoline alkaloids (BIAs) and many polyketides. An industrially tractable yeast strain optimized for pro...

    Nicholas D. Gold, Christopher M. Gowen, Francois-Xavier Lussier, Sarat C. Cautha, Radhakrishnan Mahadevan and Vincent J. J. Martin

    Microbial Cell Factories 2015 14:73

    Published on: 28 May 2015

  24. Research

    Identification of new Saccharomyces cerevisiae variants of the MET2 and SKP2 genes controlling the sulfur assimilation pathway and the production of undesirable sulfur compounds during alcoholic fermentation

    Wine yeasts can produce undesirable sulfur compounds during alcoholic fermentation, such as SO2 and H2S, in variable amounts depending mostly on the yeast strain but also on the conditions. However, although sulf...

    Jessica Noble, Isabelle Sanchez and Bruno Blondin

    Microbial Cell Factories 2015 14:68

    Published on: 8 May 2015

  25. Research

    Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production

    Terpenoids (isoprenoids) have numerous applications in flavors, fragrances, drugs and biofuels. The number of microbially produced terpenoids is increasing as new biosynthetic pathways are being elucidated. Ho...

    Fotini A Trikka, Alexandros Nikolaidis, Anastasia Athanasakoglou, Aggeliki Andreadelli, Codruta Ignea, Konstantia Kotta, Anagnostis Argiriou, Sotirios C Kampranis and Antonios M Makris

    Microbial Cell Factories 2015 14:60

    Published on: 24 April 2015

  26. Research

    Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae

    Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molec...

    Fan Yang, Zhi-Cheng Liu, Xue Wang, Li-Li Li, Lan Yang, Wen-Zhu Tang, Zhi-Min Yu and Xianzhen Li

    Microbial Cell Factories 2015 14:59

    Published on: 17 April 2015

  27. Technical Notes

    Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity

    Measurement of mitochondrial ATP synthesis is a critical way to compare cellular energetic performance. However, fractionation of mitochondria requires large amounts of cells, lengthy purification procedures, ...

    Xiaoting Ye, Kana Morikawa, Shih-Hsin Ho, Michihiro Araki, Keiji Nishida, Tomohisa Hasunuma, Kiyotaka Y Hara and Akihiko Kondo

    Microbial Cell Factories 2015 14:56

    Published on: 16 April 2015

  28. Research

    Fungal cytochrome P450 monooxygenases of Fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered Saccharomyces cerevisiae

    Omega hydroxy fatty acids (ω-OHFAs) are multifunctional compounds that act as the basis for the production of various industrial products with broad commercial and pharmaceutical implications. However, the ter...

    Pradeepraj Durairaj, Sailesh Malla, Saravanan Prabhu Nadarajan, Pyung-Gang Lee, Eunok Jung, Hyun Ho Park, Byung-Gee Kim and Hyungdon Yun

    Microbial Cell Factories 2015 14:45

    Published on: 2 April 2015

  29. Technical Notes

    Dynamic regulation of gene expression using sucrose responsive promoters and RNA interference in Saccharomyces cerevisiae

    Engineering dynamic, environmentally- and temporally-responsive control of gene expression is one of the principle objectives in the field of synthetic biology. Dynamic regulation is desirable because many eng...

    Thomas C Williams, Monica I Espinosa, Lars K Nielsen and Claudia E Vickers

    Microbial Cell Factories 2015 14:43

    Published on: 1 April 2015

  30. Research

    Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris

    The methylotrophic yeast, Pichia pastoris, is widely used as a useful experimental tool in protein engineering and production. It is common for proteins expressed in P. pastoris to exhibit N-glycosylation. In rec...

    Min Yang, Xiao-Wei Yu, Haiyan Zheng, Chong Sha, Caifeng Zhao, Meiqian Qian and Yan Xu

    Microbial Cell Factories 2015 14:40

    Published on: 21 March 2015

  31. Research

    Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production

    The bioethanol production system used in Brazil is based on the fermentation of sucrose from sugarcane feedstock by highly adapted strains of the yeast Saccharomyces cerevisiae. Bacterial contaminants present in ...

    Osmar V Carvalho-Netto, Marcelo F Carazzolle, Luciana S Mofatto, Paulo JPL Teixeira, Melline F Noronha, Luige AL Calderón, Piotr A Mieczkowski, Juan Lucas Argueso and Gonçalo AG Pereira

    Microbial Cell Factories 2015 14:13

    Published on: 30 January 2015

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