RNA: Methods and Protocols

This special collection of articles has been launched with the intention of providing novel insights into the latest methods within the field of RNA, addressing questions of general interest to the RNA community, as well as predictions on future uses, modifications and improvements that would advance existing methods beyond their current use. If you would like to submit a manuscript for the series, please use our online submission system and indicate in your cover letter that you would like it to be considered for the series; alternatively, send a pre-submission enquiry to editorial@silencejournal.com.

Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection

High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abu...

Authors: Zhao Zhang, William E Theurkauf, Zhiping Weng and Phillip D Zamore

Citation: Silence 2012 3:9

Content type: Methods Published on: 28 December 2012
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RNA: methods and protocols — a new series

This month, Silence launches a new series on methods and protocols to study silencing pathways and analyze nucleic acids and proteins.

Authors: Phillip D Zamore

Citation: Silence 2012 3:7

Content type: Editorial Published on: 7 June 2012
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Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana

Cytosine methylation is an important chromatin modification that maintains genome integrity and regulates gene expression through transcriptional gene silencing. Major players in de novo methylation guided by siR...

Authors: So Youn Won, Shengben Li, Binglian Zheng, Yuanyuan Zhao, Dongming Li, Xin Zhao, Huilan Yi, Lei Gao, Thanh Theresa Dinh and Xuemei Chen

Citation: Silence 2012 3:6

Content type: Methods Published on: 7 June 2012
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RNA: Methods and Protocols

Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection

RNA: methods and protocols — a new series

Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana