Fluorescence-based quantitative real-time PCR (qRT-PCR) is a widely and commonly used technology to quantify DNA and RNA products. The main applications of qRT-PCR are diagnostic for rapid detection of nucleic acids characteristic of infectious diseases, cancer or genetic abnormalities and, when coupled with reverse transcription, it is mainly used to provide quantitative measurements of gene transcription. The validity and reproducibility of the results of qRT-PCR studies depend on numerous factors, including but not limited to, adequate reporting of experimental settings, choice of appropriate reference genes and statistical analysis of the data generated. This topical series is a collection of manuscripts relevant to researchers interested in normalization and validation of qRT-PCR experiments.
Dr Joshua Yuan