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Recombinant protein quality: approaches for the quantitative evaluation of functional and structural features of purified proteins and antibodies

Edited by Prof Ario de Marco

Microbial Cell Factories welcomes submissions to this thematic series, covering all aspects of methodologies aimed at analyzing the preservation of the structure, solubility, and functionality of purified proteins and antibodies. Applications of new biophysical and imaging technologies are welcome, as well as the description of automated approaches. See here for further information, including how to submit.

This collection of articles has not been sponsored and articles have undergone the journal’s standard peer-review process. The Guest Editors declare no competing interests.

  1. Research

    A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis

    Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a centr...

    Dongbo Cai, Hao Wang, Penghui He, Chengjun Zhu, Qin Wang, Xuetuan Wei, Christopher T. Nomura and Shouwen Chen

    Microbial Cell Factories 2017 16:70

    Published on: 24 April 2017

  2. Research

    Elimination of N-glycosylation by site mutation further prolongs the half-life of IFN-α/Fc fusion proteins expressed in Pichia pastoris

    Interferon (IFN)-α has been commonly used as an antiviral drug worldwide; however, its short half-life in circulation due to its low molecular weight and sensitivity to proteases impacts its efficacy and patie...

    Hao Jia, Yugang Guo, Xiaoping Song, Changsheng Shao, Jing Wu, Jiajia Ma, Mingyang Shi, Yuhui Miao, Rui Li, Dong Wang, Zhigang Tian and Weihua Xiao

    Microbial Cell Factories 2016 15:209

    Published on: 7 December 2016

  3. Research

    Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

    Lactic acid is a versatile chemical platform with many different industrial applications. Yeasts have been demonstrated as attractive alternative to natural lactic acid producers since they can grow at low pH,...

    Nadia Maria Berterame, Danilo Porro, Diletta Ami and Paola Branduardi

    Microbial Cell Factories 2016 15:39

    Published on: 17 February 2016

  4. Research

    A new potential secretion pathway for recombinant proteins in Bacillus subtilis

    Secretion of cytoplasmic expressed proteins into growth media has significant advantages. Due to the lack of an outer membrane, Bacillus subtilis is considered as a desirable ‘cell factory’ for the secretion of r...

    Guangqiang Wang, Yongjun Xia, Zhennan Gu, Hao Zhang, Yong Q. Chen, Haiqin Chen, Lianzhong Ai and Wei Chen

    Microbial Cell Factories 2015 14:179

    Published on: 10 November 2015

  5. Research

    Mammalian prion protein (PrP) forms conformationally different amyloid intracellular aggregates in bacteria

    An increasing number of proteins are being shown to assemble into amyloid structures that lead to pathological states. Among them, mammalian prions outstand due to their ability to transmit the pathogenic conf...

    Bruno Macedo, Ricardo Sant’Anna, Susanna Navarro, Yraima Cordeiro and Salvador Ventura

    Microbial Cell Factories 2015 14:174

    Published on: 4 November 2015

  6. Research

    Enhanced production of a single domain antibody with an engineered stabilizing extra disulfide bond

    Single domain antibodies derived from the variable region of the unique heavy chain antibodies found in camelids yield high affinity and regenerable recognition elements. Adding an additional disulfide bond th...

    Jinny L. Liu, Ellen R. Goldman, Dan Zabetakis, Scott A. Walper, Kendrick B. Turner, Lisa C. Shriver-Lake and George P. Anderson

    Microbial Cell Factories 2015 14:158

    Published on: 9 October 2015

  7. Technical Notes

    High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG

    For membrane protein production, the Escherichia coli T7 RNA polymerase (T7 RNAP)-based protein production strain BL21(DE3) in combination with T7-promoter based expression vectors is widely used. Cells are routi...

    Zhe Zhang, Grietje Kuipers, Łukasz Niemiec, Thomas Baumgarten, Dirk Jan Slotboom, Jan-Willem de Gier and Anna Hjelm

    Microbial Cell Factories 2015 14:142

    Published on: 16 September 2015

  8. Research

    Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis

    Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host to produce proteins of ...

    Frédéric Samazan, Bachra Rokbi, Delphine Seguin, Fabienne Telles, Valérie Gautier, Gilbert Richarme, Didier Chevret, Paloma Fernández Varela, Christophe Velours and Isabelle Poquet

    Microbial Cell Factories 2015 14:104

    Published on: 16 July 2015

  9. Research

    Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins

    Lipopolysaccharide (LPS), also referred to as endotoxin, is the major constituent of the outer leaflet of the outer membrane of virtually all Gram-negative bacteria. The lipid A moiety, which anchors the LPS m...

    Uwe Mamat, Kathleen Wilke, David Bramhill, Andra Beate Schromm, Buko Lindner, Thomas Andreas Kohl, José Luis Corchero, Antonio Villaverde, Lana Schaffer, Steven Robert Head, Chad Souvignier, Timothy Charles Meredith and Ronald Wesley Woodard

    Microbial Cell Factories 2015 14:57

    Published on: 16 April 2015

    The Erratum to this article has been published in Microbial Cell Factories 2015 14:81

  10. Research

    An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

    Cell display technologies (e.g. bacterial display) are attractive in directed evolution as they provide the option to use flow-cytometric cell sorting for selection from combinatorial libraries. The aim of thi...

    Filippa Fleetwood, Ken G Andersson, Stefan Ståhl and John Löfblom

    Microbial Cell Factories 2014 13:179

    Published on: 30 December 2014

  11. Review

    Quality assessment and optimization of purified protein samples: why and how?

    Purified protein quality control is the final and critical check-point of any protein production process. Unfortunately, it is too often overlooked and performed hastily, resulting in irreproducible and mislea...

    Bertrand Raynal, Pascal Lenormand, Bruno Baron, Sylviane Hoos and Patrick England

    Microbial Cell Factories 2014 13:180

    Published on: 30 December 2014

  12. Research

    Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies

    The isolation of recombinant antibody fragments from displayed libraries represents a powerful alternative to the generation of IgGs using hybridoma technology. The selected antibody fragments can then be easi...

    Selma Djender, Aurelie Schneider, Anne Beugnet, Ronan Crepin, Klervi Even Desrumeaux, Chiara Romani, Sandrine Moutel, Franck Perez and Ario de Marco

    Microbial Cell Factories 2014 13:140

    Published on: 16 September 2014

  13. Research

    Influence of pH control in the formation of inclusion bodies during production of recombinant sphingomyelinase-D in Escherichia coli

    Inclusion bodies (IBs) are aggregated proteins that form clusters when protein is overexpressed in heterologous expression systems. IBs have been considered as non-usable proteins, but recently they are being ...

    Andrea Castellanos-Mendoza, Ricardo M Castro-Acosta, Alejandro Olvera, Guadalupe Zavala, Miguel Mendoza-Vera, Enrique García-Hernández, Alejandro Alagón, Mauricio A Trujillo-Roldán and Norma A Valdez-Cruz

    Microbial Cell Factories 2014 13:137

    Published on: 12 September 2014

  14. Research

    Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris

    Recombinant antibodies are highly successful in many different pathological conditions and currently enjoy overwhelming recognition of their potential. There are a wide variety of protein expression systems av...

    Ana Blanco-Toribio, Javier Lacadena, Natalia Nuñez-Prado, Ana Álvarez-Cienfuegos, Maider Villate, Marta Compte, Laura Sanz, Francisco J Blanco and Luis Álvarez-Vallina

    Microbial Cell Factories 2014 13:116

    Published on: 12 August 2014

  15. Research

    Effect of metal catalyzed oxidation in recombinant viral protein assemblies

    Protein assemblies, such as virus-like particles, have increasing importance as vaccines, delivery vehicles and nanomaterials. However, their use requires stable assemblies. An important cause of loss of stabi...

    Ricardo M Castro-Acosta, William A Rodríguez-Limas, Brenda Valderrama, Octavio T Ramírez and Laura A Palomares

    Microbial Cell Factories 2014 13:25

    Published on: 17 February 2014

  16. Research

    Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins

    A number of valuable candidates as tuberculosis vaccine have been reported, some of which have already entered clinical trials. The new vaccines, especially subunit vaccines, need multiple administrations in o...

    Luciano Piubelli, Manuela Campa, Caterina Temporini, Elisa Binda, Francesca Mangione, Massimo Amicosante, Marco Terreni, Flavia Marinelli and Loredano Pollegioni

    Microbial Cell Factories 2013 12:115

    Published on: 19 November 2013

  17. Commentary

    Why and how protein aggregation has to be studied in vivo

    The understanding of protein aggregation is a central issue in different fields of protein science, from the heterologous protein production in biotechnology to amyloid aggregation in several neurodegenerative...

    Diletta Ami, Antonino Natalello, Marina Lotti and Silvia Maria Doglia

    Microbial Cell Factories 2013 12:17

    Published on: 15 February 2013