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Recombinant protein quality: approaches for the quantitative evaluation of functional and structural features of purified proteins and antibodies

Edited by Prof Ario de Marco

Microbial Cell Factories welcomes submissions to this thematic series, covering all aspects of methodologies aimed at analyzing the preservation of the structure, solubility, and functionality of purified proteins and antibodies. Applications of new biophysical and imaging technologies are welcome, as well as the description of automated approaches. See here for further information, including how to submit.

This collection of articles has not been sponsored and articles have undergone the journal’s standard peer-review process. The Guest Editors declare no competing interests.

  1. Content type: Research

    The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient...

    Authors: Milica Popovic, Elisa Mazzega, Barbara Toffoletto and Ario de Marco

    Citation: Microbial Cell Factories 2018 17:6

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  2. Content type: Research

    Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a centr...

    Authors: Dongbo Cai, Hao Wang, Penghui He, Chengjun Zhu, Qin Wang, Xuetuan Wei, Christopher T. Nomura and Shouwen Chen

    Citation: Microbial Cell Factories 2017 16:70

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  3. Content type: Research

    Interferon (IFN)-α has been commonly used as an antiviral drug worldwide; however, its short half-life in circulation due to its low molecular weight and sensitivity to proteases impacts its efficacy and patie...

    Authors: Hao Jia, Yugang Guo, Xiaoping Song, Changsheng Shao, Jing Wu, Jiajia Ma, Mingyang Shi, Yuhui Miao, Rui Li, Dong Wang, Zhigang Tian and Weihua Xiao

    Citation: Microbial Cell Factories 2016 15:209

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  4. Content type: Research

    Lactic acid is a versatile chemical platform with many different industrial applications. Yeasts have been demonstrated as attractive alternative to natural lactic acid producers since they can grow at low pH,...

    Authors: Nadia Maria Berterame, Danilo Porro, Diletta Ami and Paola Branduardi

    Citation: Microbial Cell Factories 2016 15:39

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  5. Content type: Research

    Disulfide bonds are the most common structural, post-translational modification found in proteins. Antibodies contain up to 25 disulfide bonds depending on type, with sc...

    Authors: Anna Gaciarz, Johanna Veijola, Yuko Uchida, Mirva J. Saaranen, Chunguang Wang, Sohvi Hörkkö and Lloyd W. Ruddock

    Citation: Microbial Cell Factories 2016 15:22

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  6. Content type: Research

    Secretion of cytoplasmic expressed proteins into growth media has significant advantages. Due to the lack of an outer membrane, Bacillus subtilis is considered as a desirable ‘cell factory’ for the secretion of r...

    Authors: Guangqiang Wang, Yongjun Xia, Zhennan Gu, Hao Zhang, Yong Q. Chen, Haiqin Chen, Lianzhong Ai and Wei Chen

    Citation: Microbial Cell Factories 2015 14:179

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  7. Content type: Research

    An increasing number of proteins are being shown to assemble into amyloid structures that lead to pathological states. Among them, mammalian prions outstand due to their ability to transmit the pathogenic conf...

    Authors: Bruno Macedo, Ricardo Sant’Anna, Susanna Navarro, Yraima Cordeiro and Salvador Ventura

    Citation: Microbial Cell Factories 2015 14:174

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  8. Content type: Research

    Single domain antibodies derived from the variable region of the unique heavy chain antibodies found in camelids yield high affinity and regenerable recognition elements. Adding an additional disulfide bond th...

    Authors: Jinny L. Liu, Ellen R. Goldman, Dan Zabetakis, Scott A. Walper, Kendrick B. Turner, Lisa C. Shriver-Lake and George P. Anderson

    Citation: Microbial Cell Factories 2015 14:158

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  9. Content type: Technical Notes

    For membrane protein production, the Escherichia coli T7 RNA polymerase (T7 RNAP)-based protein production strain BL21(DE3) in combination with T7-promoter based expression vectors is widely used. Cells are routi...

    Authors: Zhe Zhang, Grietje Kuipers, Łukasz Niemiec, Thomas Baumgarten, Dirk Jan Slotboom, Jan-Willem de Gier and Anna Hjelm

    Citation: Microbial Cell Factories 2015 14:142

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  10. Content type: Research

    Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host to produce proteins of ...

    Authors: Frédéric Samazan, Bachra Rokbi, Delphine Seguin, Fabienne Telles, Valérie Gautier, Gilbert Richarme, Didier Chevret, Paloma Fernández Varela, Christophe Velours and Isabelle Poquet

    Citation: Microbial Cell Factories 2015 14:104

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  11. Content type: Research

    The formation of protein inclusions is connected to the onset of many human diseases. Human RNA binding proteins containing intrinsically disordered regions with an amino acid composition resembling those of y...

    Authors: Susanna Navarro, Patrizia Marinelli, Marta Diaz-Caballero and Salvador Ventura

    Citation: Microbial Cell Factories 2015 14:102

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  12. Content type: Research

    Lipopolysaccharide (LPS), also referred to as endotoxin, is the major constituent of the outer leaflet of the outer membrane of virtually all Gram-negative bacteria. The lipid A moiety, which anchors the LPS m...

    Authors: Uwe Mamat, Kathleen Wilke, David Bramhill, Andra Beate Schromm, Buko Lindner, Thomas Andreas Kohl, José Luis Corchero, Antonio Villaverde, Lana Schaffer, Steven Robert Head, Chad Souvignier, Timothy Charles Meredith and Ronald Wesley Woodard

    Citation: Microbial Cell Factories 2015 14:57

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    The Erratum to this article has been published in Microbial Cell Factories 2015 14:81

  13. Content type: Research

    The hERG potassium channel is essential for repolarization of the cardiac action potential. Due to this vital function, absence of unintended and potentially life-threatening interactions with hERG is ...

    Authors: Karen Molbaek, Peter Scharff-Poulsen, Claus Helix-Nielsen, Dan A Klaerke and Per Amstrup Pedersen

    Citation: Microbial Cell Factories 2015 14:15

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  14. Content type: Research

    Recombinant protein-based therapeutics have become indispensable for the treatment of many diseases. They are produced using well-established expression systems based on bacteria, yeast, insect and mammalian c...

    Authors: Jennifer Lauber, René Handrick, Sebastian Leptihn, Peter Dürre and Sabine Gaisser

    Citation: Microbial Cell Factories 2015 14:3

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  15. Content type: Research

    Amphiphilic peptides are important building blocks to generate nanostructured biomaterials for drug delivery and tissue engineering applications. We have shown that the self-assembling peptide SA2 (Ac-AAVVLLLW...

    Authors: Mazda Rad-Malekshahi, Matthias Flement, Wim E Hennink and Enrico Mastrobattista

    Citation: Microbial Cell Factories 2014 13:178

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  16. Content type: Review

    Purified protein quality control is the final and critical check-point of any protein production process. Unfortunately, it is too often overlooked and performed hastily, resulting in irreproducible and mislea...

    Authors: Bertrand Raynal, Pascal Lenormand, Bruno Baron, Sylviane Hoos and Patrick England

    Citation: Microbial Cell Factories 2014 13:180

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  17. Content type: Research

    Cell display technologies (e.g. bacterial display) are attractive in directed evolution as they provide the option to use flow-cytometric cell sorting for selection from combinatorial libraries. The aim of thi...

    Authors: Filippa Fleetwood, Ken G Andersson, Stefan Ståhl and John Löfblom

    Citation: Microbial Cell Factories 2014 13:179

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  18. Content type: Research

    The isolation of recombinant antibody fragments from displayed libraries represents a powerful alternative to the generation of IgGs using hybridoma technology. The selected antibody fragments can then be easi...

    Authors: Selma Djender, Aurelie Schneider, Anne Beugnet, Ronan Crepin, Klervi Even Desrumeaux, Chiara Romani, Sandrine Moutel, Franck Perez and Ario de Marco

    Citation: Microbial Cell Factories 2014 13:140

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  19. Content type: Research

    Inclusion bodies (IBs) are aggregated proteins that form clusters when protein is overexpressed in heterologous expression systems. IBs have been considered as non-usable proteins, but recently they are being ...

    Authors: Andrea Castellanos-Mendoza, Ricardo M Castro-Acosta, Alejandro Olvera, Guadalupe Zavala, Miguel Mendoza-Vera, Enrique García-Hernández, Alejandro Alagón, Mauricio A Trujillo-Roldán and Norma A Valdez-Cruz

    Citation: Microbial Cell Factories 2014 13:137

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  20. Content type: Research

    Recombinant antibodies are highly successful in many different pathological conditions and currently enjoy overwhelming recognition of their potential. There are a wide variety of protein expression systems av...

    Authors: Ana Blanco-Toribio, Javier Lacadena, Natalia Nuñez-Prado, Ana Álvarez-Cienfuegos, Maider Villate, Marta Compte, Laura Sanz, Francisco J Blanco and Luis Álvarez-Vallina

    Citation: Microbial Cell Factories 2014 13:116

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  21. Content type: Research

    Protein assemblies, such as virus-like particles, have increasing importance as vaccines, delivery vehicles and nanomaterials. However, their use requires stable assemblies. An important cause of loss of stabi...

    Authors: Ricardo M Castro-Acosta, William A Rodríguez-Limas, Brenda Valderrama, Octavio T Ramírez and Laura A Palomares

    Citation: Microbial Cell Factories 2014 13:25

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  22. Content type: Research

    A number of valuable candidates as tuberculosis vaccine have been reported, some of which have already entered clinical trials. The new vaccines, especially subunit vaccines, need multiple administrations in o...

    Authors: Luciano Piubelli, Manuela Campa, Caterina Temporini, Elisa Binda, Francesca Mangione, Massimo Amicosante, Marco Terreni, Flavia Marinelli and Loredano Pollegioni

    Citation: Microbial Cell Factories 2013 12:115

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  23. Content type: Research

    Hepatitis C virus (HCV) is a common and leading cause for liver cirrhosis and hepatocellular carcinoma. Current therapies to treat HCV infection are shown to be partially effective and poorly tolerated. Theref...

    Authors: Muhammad Ikram Anwar, Mazhar Iqbal, Mohammad S Yousef and Moazur Rahman

    Citation: Microbial Cell Factories 2013 12:111

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  24. Content type: Technical Notes

    Monoterpenes are a class of natural C10 compounds with a range of potential applications including use as fuel additives, fragrances, and chemical feedstocks. Biosynthesis of monoterpenes in heterologous systems ...

    Authors: James BYH Behrendorff, Claudia E Vickers, Panagiotis Chrysanthopoulos and Lars K Nielsen

    Citation: Microbial Cell Factories 2013 12:76

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  25. Content type: Research

    Biologically active inclusion bodies (IBs) have gained much attention in recent years. Fusion with IB-inducing partner has been shown to be an efficient strategy for generating active IBs. To make full use of ...

    Authors: Ziliang Huang, Chong Zhang, Shuo Chen, Fengchun Ye and Xin-Hui Xing

    Citation: Microbial Cell Factories 2013 12:25

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  26. Content type: Commentary

    The understanding of protein aggregation is a central issue in different fields of protein science, from the heterologous protein production in biotechnology to amyloid aggregation in several neurodegenerative...

    Authors: Diletta Ami, Antonino Natalello, Marina Lotti and Silvia Maria Doglia

    Citation: Microbial Cell Factories 2013 12:17

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