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Plant Genome Editing

Plant Methods

© Schiml and Puchta. 2016The exciting field of genome editing is rapidly advancing and precise genome editing techniques have already become an important tool for both fundamental research and plant biotechnology.

This collection includes both invited reviews and unsolicited research covering all aspects of genome editing technologies as applied to plant research (both for crop plants and model organisms).

This collection is published in conjunction with the GARNet-OpenPlant CRISPR-Cas Workshop held at the John Innes Centre Norwich on the 7-8 September 2015.

If you are interested in contributing to this series you can do so via our online submission system. If you are uncertain about the suitability of a manuscript for consideration, please feel free to contact the Editors at

  1. Reduction in rice yield caused by high temperature-induced spikelet sterility has been a serious concern in rice production. To date, several screening methods have been used, although their reproducibility i...

    Authors: Makoto Hakata, Hiroshi Wada, Chisato Masumoto-Kubo, Ryo Tanaka, Hiroyuki Sato and Satoshi Morita
    Citation: Plant Methods 2017 13:34
  2. The efficiency of multiplex editing in plants by the RNA-guided Cas9 system is limited by efficient introduction of its components into the genome and by their activity. The possibility of introducing large fragm...

    Authors: Wenhao Yan, Dijun Chen and Kerstin Kaufmann
    Citation: Plant Methods 2016 12:23
  3. Although customized endonucleases [transcription activator-like effector nucleases (TALENs) and RNA-guided endonucleases (RGENs)] are known to be effective agents of mutagenesis in various host plants, newly d...

    Authors: Nagaveni Budhagatapalli, Sindy Schedel, Maia Gurushidze, Stefanie Pencs, Stefan Hiekel, Twan Rutten, Stefan Kusch, Robert Morbitzer, Thomas Lahaye, Ralph Panstruga, Jochen Kumlehn and Goetz Hensel
    Citation: Plant Methods 2016 12:18
  4. The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the constructi...

    Authors: Marta Vazquez-Vilar, Joan Miquel Bernabé-Orts, Asun Fernandez-del-Carmen, Pello Ziarsolo, Jose Blanca, Antonio Granell and Diego Orzaez
    Citation: Plant Methods 2016 12:10
  5. Targeted genome engineering has been described as a “game-changing technology” for fields as diverse as human genetics and plant biotechnology. One technique used for precise gene editing utilises the CRISPR-C...

    Authors: Geraint Parry, Nicola Patron, Ruth Bastow and Colette Matthewman
    Citation: Plant Methods 2016 12:6