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Plant Genome Editing

Plant Methods

© Schiml and Puchta. 2016The exciting field of genome editing is rapidly advancing and precise genome editing techniques have already become an important tool for both fundamental research and plant biotechnology.

This collection includes both invited reviews and unsolicited research covering all aspects of genome editing technologies as applied to plant research (both for crop plants and model organisms).

This collection is published in conjunction with the GARNet-OpenPlant CRISPR-Cas Workshop held at the John Innes Centre Norwich on the 7-8 September 2015.

If you are interested in contributing to this series you can do so via our online submission system. If you are uncertain about the suitability of a manuscript for consideration, please feel free to contact the Editors at

  1. Methodology

    Development of a new heat tolerance assay system for rice spikelet sterility

    Reduction in rice yield caused by high temperature-induced spikelet sterility has been a serious concern in rice production. To date, several screening methods have been used, although their reproducibility i...

    Makoto Hakata, Hiroshi Wada, Chisato Masumoto-Kubo, Ryo Tanaka, Hiroyuki Sato and Satoshi Morita

    Plant Methods 2017 13:34

    Published on: 10 May 2017

  2. Research

    A simple test for the cleavage activity of customized endonucleases in plants

    Although customized endonucleases [transcription activator-like effector nucleases (TALENs) and RNA-guided endonucleases (RGENs)] are known to be effective agents of mutagenesis in various host plants, newly d...

    Nagaveni Budhagatapalli, Sindy Schedel, Maia Gurushidze, Stefanie Pencs, Stefan Hiekel, Twan Rutten, Stefan Kusch, Robert Morbitzer, Thomas Lahaye, Ralph Panstruga, Jochen Kumlehn and Goetz Hensel

    Plant Methods 2016 12:18

    Published on: 9 March 2016

  3. Methodology

    A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard

    The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the constructi...

    Marta Vazquez-Vilar, Joan Miquel Bernabé-Orts, Asun Fernandez-del-Carmen, Pello Ziarsolo, Jose Blanca, Antonio Granell and Diego Orzaez

    Plant Methods 2016 12:10

    Published on: 1 February 2016

  4. Commentary

    Meeting report: GARNet/OpenPlant CRISPR-Cas workshop

    Targeted genome engineering has been described as a “game-changing technology” for fields as diverse as human genetics and plant biotechnology. One technique used for precise gene editing utilises the CRISPR-C...

    Geraint Parry, Nicola Patron, Ruth Bastow and Colette Matthewman

    Plant Methods 2016 12:6

    Published on: 27 January 2016