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Next Generation Sequencing technologies for plant research

A joint GARNet-Genetics Society meeting on “New Technologies to Advance Plant Research”, held in November 2012 at Liverpool University, was the catalyst for this special Thematic Series on Next Generation Sequencing Technologies for Plant Research. Next-generation sequencing (NGS) encompasses a number of different sequencing technologies (e.g. Illumina/Solexa, Roche 454, Ion torrent and SOLiD sequencing) that share the feature of allowing us to sequence DNA and RNA much more quickly and cheaply than Sanger sequencing. These new massively parallel sequencing technologies are revolutionising molecular biology by providing insights into the genome, the transcriptome and the epigenome on an unprecedented scale. The thematic series aims to cover all aspects of the application of new sequencing technologies to plant research - and new submissions (including Methodology papers) will be encouraged until 31st December 2013.

  1. Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within ...

    Authors: Adam Healey, Agnelo Furtado, Tal Cooper and Robert J Henry
    Citation: Plant Methods 2014 10:21
    Content type: Methodology Published on:

  3. Molecular profiling of gene families is a versatile tool to study diversity between individual genomes in sexual crosses and germplasm. Nucleotide binding site (NBS) profiling, in particular, targets conserved...

    Authors: Jack H Vossen, Sara Dezhsetan, Danny Esselink, Marjon Arens, Maria J Sanz, Walter Verweij, Estelle Verzaux and C Gerard van der Linden
    Citation: Plant Methods 2013 9:37
    Content type: Methodology Published on:

  4. The genomic sequences of many important Triticeae crop species are hard to assemble and analyse due to their large genome sizes, (in part) polyploid genomes and high repeat content. Recently, the draft genomes of...

    Authors: Manuel Spannagl, Mihaela M Martis, Matthias Pfeifer, Thomas Nussbaumer and Klaus FX Mayer
    Citation: Plant Methods 2013 9:35
    Content type: Review Published on: