Guest Editors: Barbara Wold and Kin Fai Au
In this new special issue in Genome Biology, we present a series of papers using Long-read sequencing. Long-read sequencing technologies provide unprecedented opportunities to solve many complex biomedical problems, especially for those unsolved by short-read sequencing. Their long read lengths offer unambiguous mappability for reliable identification of genomic events and are also informative for long-range analyses, such as haplotyping and genome assembly. In addition, the long-read techniques, PacBio and Oxford Nanopore Technologies sequence single molecules and can detect canonical nucleotides as well as base modifications. Both experimental and computational method developments are needed to fully utilize long-read sequencing while accounting for their corresponding weakness (e.g. higher error rates). To translate the technological advances into biomedical breakthroughs, other avenues of research are exploring innovative applications of long-read sequencing to significant biomedical contexts.
(Image by Jane Cha at the Jackson Laboratory)