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Yeast biotechnology

Edited by Prof Diethard Mattanovich

Microbial Cell Factories invites submissions to the recently launched thematic series on “Yeast biotechnology”.

Mankind has exploited the metabolic activities of yeasts in baking and brewing for several millennia. The invention of the fed-batch technology for production of bakers' yeast has been a cornerstone of modern biotechnology, enabling the development of efficient production processes for antibiotics, biopharmaceuticals and technical enzymes. Alcoholic fermentation, on the other hand, is a model for microbial metabolite production and has become a major research focus for the manufacturing of biofuels. Expanding from our knowledge on bakers' yeast, diverse yeast species are explored today. The series highlights the latest research to produce biopharmaceuticals, enzymes, alcohols, organic acids, flavours and fine chemicals, underlining the huge potential of yeast biotechnology.

This collection of articles has not been sponsored and articles have undergone the journal’s standard peer-review process. The Guest Editors declare no competing interests.

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  1. Content type: Research

    Cultivation of recombinant Pichia pastoris (Komagataella sp.) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expres...

    Authors: Núria Adelantado, Pablo Tarazona, Karlheinz Grillitsch, Xavier García-Ortega, Sergi Monforte, Francisco Valero, Ivo Feussner, Günther Daum and Pau Ferrer

    Citation: Microbial Cell Factories 2017 16:86

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  2. Content type: Research

    Bacterial inclusion bodies (IBs) are non-toxic protein aggregates commonly produced in recombinant bacteria. They are formed by a mixture of highly stable amyloid-like fibrils and releasable protein species wi...

    Authors: Fabián Rueda, Brigitte Gasser, Alejandro Sánchez-Chardi, Mònica Roldán, Sandra Villegas, Verena Puxbaum, Neus Ferrer-Miralles, Ugutz Unzueta, Esther Vázquez, Elena Garcia-Fruitós, Diethard Mattanovich and Antonio Villaverde

    Citation: Microbial Cell Factories 2016 15:166

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  3. Content type: Research

    Glycosylation of flavonoids is a promising approach to improve the pharmacokinetic properties and biological activities of flavonoids. Recently, many efforts such as enzymatic biocatalysis and the engineered Esch...

    Authors: Huimin Wang, Yan Yang, Lin Lin, Wenlong Zhou, Minzhi Liu, Kedi Cheng and Wei Wang

    Citation: Microbial Cell Factories 2016 15:134

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  4. Content type: Research

    The yeast amino acid catabolism plays an important role in flavour generation since higher alcohols and acetate esters, amino acid catabolism end products, are key components of overall flavour and aroma in fe...

    Authors: Jiri Stribny, Gabriele Romagnoli, Roberto Pérez-Torrado, Jean-Marc Daran and Amparo Querol

    Citation: Microbial Cell Factories 2016 15:51

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  5. Content type: Research

    Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combine...

    Authors: Danna Lee, Natoiya D. R. Lloyd, Isak S. Pretorius and Anthony R. Borneman

    Citation: Microbial Cell Factories 2016 15:49

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  6. Content type: Research

    With increasing concern about the environmental impact of a petroleum based economy, focus has shifted towards greener production strategies including metabolic engineering of microbes for the conversion of pl...

    Authors: Sarah Rodriguez, Charles M. Denby, T. Van Vu, Edward E. K. Baidoo, George Wang and Jay D. Keasling

    Citation: Microbial Cell Factories 2016 15:48

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  7. Content type: Research

    Several plant diterpenes have important biological properties. Among them, forskolin is a complex labdane-type diterpene whose biological activity stems from its ability to activate adenylyl cyclase and to ele...

    Authors: Codruta Ignea, Efstathia Ioannou, Panagiota Georgantea, Fotini A. Trikka, Anastasia Athanasakoglou, Sofia Loupassaki, Vassilios Roussis, Antonios M. Makris and Sotirios C. Kampranis

    Citation: Microbial Cell Factories 2016 15:46

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  8. Content type: Research

    Lactic acid is a versatile chemical platform with many different industrial applications. Yeasts have been demonstrated as attractive alternative to natural lactic acid producers since they can grow at low pH,...

    Authors: Nadia Maria Berterame, Danilo Porro, Diletta Ami and Paola Branduardi

    Citation: Microbial Cell Factories 2016 15:39

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  9. Content type: Research

    Saccharomyces cerevisiae can be engineered to perform a multitude of different chemical reactions that are not programmed in its original genetic code. It has a large potential to func...

    Authors: Jan Dines Knudsen, Cecilia Hägglöf, Nora Weber and Magnus Carlquist

    Citation: Microbial Cell Factories 2016 15:37

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  10. Content type: Research

    Fermentative aromas play a key role in the organoleptic profile of young wines. Their production depends both on yeast strain and fermentation conditions. A present-day trend in the wine industry consists in d...

    Authors: Stéphanie Rollero, Jean-Roch Mouret, Isabelle Sanchez, Carole Camarasa, Anne Ortiz-Julien, Jean-Marie Sablayrolles and Sylvie Dequin

    Citation: Microbial Cell Factories 2016 15:32

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  11. Content type: Research

    Imidazolium ionic liquids (IILs) underpin promising technologies that generate fermentable sugars from lignocellulose for future biorefineries. However, residual IILs ar...

    Authors: Quinn Dickinson, Scott Bottoms, Li Hinchman, Sean McIlwain, Sheena Li, Chad L. Myers, Charles Boone, Joshua J. Coon, Alexander Hebert, Trey K. Sato, Robert Landick and Jeff S. Piotrowski

    Citation: Microbial Cell Factories 2016 15:17

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  12. Content type: Commentary

    Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interes...

    Authors: Brigitte Gasser, Matthias G. Steiger and Diethard Mattanovich

    Citation: Microbial Cell Factories 2015 14:196

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  13. Content type: Research

    Lignocellulosic substrates and pulping process streams are of increasing relevance to biorefineries for second generation biofuels and biochemical production. They are known to be rich in sugars and inhibitors...

    Authors: Peter Temitope Adeboye, Maurizio Bettiga, Fredrik Aldaeus, Per Tomas Larsson and Lisbeth Olsson

    Citation: Microbial Cell Factories 2015 14:149

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  14. Content type: Research

    Glutathione (GSH), a pivotal non-protein thiol, can be biosynthesized through three pathways in different organisms: (1) two consecutive enzymatic reactions catalyzed by γ-glutamylcysteine synthetase (Gsh1 or ...

    Authors: Liang Tang, Weiwei Wang, Wenlong Zhou, Kai Cheng, Yan Yang, Minzhi Liu, Kedi Cheng and Wei Wang

    Citation: Microbial Cell Factories 2015 14:139

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  15. Content type: Research

    Monoterpenes are important contributors to grape and wine aroma. Moreover, certain monoterpenes have been shown to display health benefits with antimicrobial, anti-inflammatory, anticancer or hypotensive prope...

    Authors: Ester Pardo, Juan Rico, José Vicente Gil and Margarita Orejas

    Citation: Microbial Cell Factories 2015 14:136

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  16. Content type: Research

    Saccharomyces cerevisiae has become a popular host for production of non-native compounds. The metabolic pathways involved generally require a n...

    Authors: Tim Vos, Pilar de la Torre Cortés, Walter M. van Gulik, Jack T. Pronk and Pascale Daran-Lapujade

    Citation: Microbial Cell Factories 2015 14:133

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  17. Content type: Research

    Aroma is one of the most important attributes defining wine quality in which yeasts play a crucial role, synthesizing aromatic compounds or releasing odourless conjugate...

    Authors: Amparo Gamero, Carmela Belloch and Amparo Querol

    Citation: Microbial Cell Factories 2015 14:128

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  18. Content type: Research

    The introduction of yeast starter cultures consisting in a blend of Saccharomyces cerevisiae and non-Saccharomyces yeast strains is emerging for...

    Authors: Catarina Barbosa, Arlete Mendes-Faia, Patrícia Lage, Nuno P. Mira and Ana Mendes-Ferreira

    Citation: Microbial Cell Factories 2015 14:124

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  19. Content type: Research

    A Saccharomyces cerevisiae strain carrying deletions in all three pyruvate decarboxylase (PDC) genes (also called Pdc negative yeast) represents a non-ethanol producing platform strain for the production of pyruv...

    Authors: Yiming Zhang, Guodong Liu, Martin K M Engqvist, Anastasia Krivoruchko, Björn M Hallström, Yun Chen, Verena Siewers and Jens Nielsen

    Citation: Microbial Cell Factories 2015 14:116

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  20. Content type: Research

    Sugar alcohols have been widely applied in the fields of food and medicine owing to their unique properties. Compared to chemical production, microbial production of sugar alcohols has become attractive becaus...

    Authors: Guoqiang Zhang, Yuping Lin, Xianni Qi, Lixian Wang, Peng He, Qinhong Wang and Yanhe Ma

    Citation: Microbial Cell Factories 2015 14:112

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  21. Content type: Research

    Membrane proteins are important drug targets in many human diseases and gathering structural information regarding these proteins encourages the pharmaceutical industry ...

    Authors: Augusto Q Pedro, Luís M Martins, João M L Dias, Maria J Bonifácio, João A Queiroz and Luís A Passarinha

    Citation: Microbial Cell Factories 2015 14:113

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  22. Content type: Research

    Microbial population dynamics in bioreactors depend on both nutrients availability and changes in the growth environment. Research is still ongoing on the optimization o...

    Authors: Stefano Mazzoleni, Carmine Landi, Fabrizio Cartenì, Elisabetta de Alteriis, Francesco Giannino, Lucia Paciello and Palma Parascandola

    Citation: Microbial Cell Factories 2015 14:109

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  23. Content type: Research

    Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glyce...

    Authors: Simona Capone, Jernej Horvat, Christoph Herwig and Oliver Spadiut

    Citation: Microbial Cell Factories 2015 14:101

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  24. Content type: Research

    Cold stress reduces microbial growth and metabolism being relevant in industrial processes like wine making and brewing. Knowledge on the cold transcriptional response of S...

    Authors: Lidia Ballester-Tomás, Francisca Randez-Gil, Roberto Pérez-Torrado and Jose Antonio Prieto

    Citation: Microbial Cell Factories 2015 14:100

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  25. Content type: Research

    One of the bottlenecks in production of biochemicals and pharmaceuticals in Saccharomyces cerevisiae is stable and homogeneous expression of pathway genes. Integration of genes into the genome of the production o...

    Authors: Carlotta Ronda, Jérôme Maury, Tadas Jakočiu̅nas, Simo Abdessamad Baallal Jacobsen, Susanne Manuela Germann, Scott James Harrison, Irina Borodina, Jay D Keasling, Michael Krogh Jensen and Alex Toftgaard Nielsen

    Citation: Microbial Cell Factories 2015 14:97

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  26. Content type: Review

    The yeast Saccharomyces cerevisiae is one of the oldest and most frequently used microorganisms in biotechnology with successful applications in the production of both bulk and fine chemicals. Yet, yeast research...

    Authors: Martin Kavšček, Martin Stražar, Tomaž Curk, Klaus Natter and Uroš Petrovič

    Citation: Microbial Cell Factories 2015 14:94

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  27. Content type: Technical Notes

    Predictable control of gene expression is necessary for the rational design and optimization of cell factories. In the yeast Saccharomyces cerevisiae, the promoter is one of the most ...

    Authors: Bingyin Peng, Thomas C Williams, Matthew Henry, Lars K Nielsen and Claudia E Vickers

    Citation: Microbial Cell Factories 2015 14:91

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  28. Content type: Research

    A moderate-temperature, astaxanthin-overproducing mutant strain (termed MK19) of Phaffia rhodozyma was generated in our laboratory. The intracellular astaxanthin content of MK19 was 17-fold higher than that of wi...

    Authors: Shuang Chi, Yanfeng He, Jie Ren, Qian Su, Xingchao Liu, Zhi Chen, Mingan Wang, Ying Li and Jilun Li

    Citation: Microbial Cell Factories 2015 14:90

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  29. Content type: Research

    Apocarotenoids, like the C13-norisoprenoids, are natural compounds that contribute to the flavor and/or aroma of flowers and foods. They are produced in aromatic plants—like raspberries and roses—by the enzyma...

    Authors: Javiera López, Karen Essus, Il-kwon Kim, Rui Pereira, Jan Herzog, Verena Siewers, Jens Nielsen and Eduardo Agosin

    Citation: Microbial Cell Factories 2015 14:84

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  30. Content type: Research

    L-tyrosine is a common precursor for a wide range of valuable secondary metabolites, including benzylisoquinoline alkaloids (BIAs) and many polyketides. An industrially tractable yeast strain optimized for pro...

    Authors: Nicholas D. Gold, Christopher M. Gowen, Francois-Xavier Lussier, Sarat C. Cautha, Radhakrishnan Mahadevan and Vincent J. J. Martin

    Citation: Microbial Cell Factories 2015 14:73

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  31. Content type: Research

    Wine yeasts can produce undesirable sulfur compounds during alcoholic fermentation, such as SO2 and H2S, in variable amounts depending mostly on the yeast strain but also on the conditions. However, although sulf...

    Authors: Jessica Noble, Isabelle Sanchez and Bruno Blondin

    Citation: Microbial Cell Factories 2015 14:68

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  32. Content type: Research

    Isobutanol is an important biorefinery target alcohol that can be used as a fuel, fuel additive, or commodity chemical. Baker’s yeast, Saccharomyces cerevisiae, is a promising organism ...

    Authors: Kengo Ida, Jun Ishii, Fumio Matsuda, Takashi Kondo and Akihiko Kondo

    Citation: Microbial Cell Factories 2015 14:62

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  33. Content type: Research

    Terpenoids (isoprenoids) have numerous applications in flavors, fragrances, drugs and biofuels. The number of microbially produced terpenoids is increasing as new biosynthetic pathways are being elucidated. Ho...

    Authors: Fotini A Trikka, Alexandros Nikolaidis, Anastasia Athanasakoglou, Aggeliki Andreadelli, Codruta Ignea, Konstantia Kotta, Anagnostis Argiriou, Sotirios C Kampranis and Antonios M Makris

    Citation: Microbial Cell Factories 2015 14:60

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  34. Content type: Research

    Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molec...

    Authors: Fan Yang, Zhi-Cheng Liu, Xue Wang, Li-Li Li, Lan Yang, Wen-Zhu Tang, Zhi-Min Yu and Xianzhen Li

    Citation: Microbial Cell Factories 2015 14:59

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  35. Content type: Technical Notes

    Measurement of mitochondrial ATP synthesis is a critical way to compare cellular energetic performance. However, fractionation of mitochondria requires large amounts of cells, lengthy purification procedures, ...

    Authors: Xiaoting Ye, Kana Morikawa, Shih-Hsin Ho, Michihiro Araki, Keiji Nishida, Tomohisa Hasunuma, Kiyotaka Y Hara and Akihiko Kondo

    Citation: Microbial Cell Factories 2015 14:56

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  36. Content type: Research

    Omega hydroxy fatty acids (ω-OHFAs) are multifunctional compounds that act as the basis for the production of various industrial products with broad commercial and pharmaceutical implications. However, the ter...

    Authors: Pradeepraj Durairaj, Sailesh Malla, Saravanan Prabhu Nadarajan, Pyung-Gang Lee, Eunok Jung, Hyun Ho Park, Byung-Gee Kim and Hyungdon Yun

    Citation: Microbial Cell Factories 2015 14:45

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  37. Content type: Technical Notes

    Engineering dynamic, environmentally- and temporally-responsive control of gene expression is one of the principle objectives in the field of synthetic biology. Dynamic regulation is desirable because many eng...

    Authors: Thomas C Williams, Monica I Espinosa, Lars K Nielsen and Claudia E Vickers

    Citation: Microbial Cell Factories 2015 14:43

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  38. Content type: Research

    The methylotrophic yeast, Pichia pastoris, is widely used as a useful experimental tool in protein engineering and production. It is common for proteins expressed in P. pastoris to exhibit N-glycosylation. In rec...

    Authors: Min Yang, Xiao-Wei Yu, Haiyan Zheng, Chong Sha, Caifeng Zhao, Meiqian Qian and Yan Xu

    Citation: Microbial Cell Factories 2015 14:40

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  39. Content type: Research

    Targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the N-terminus of a secretory protein. These signal sequences usually contain an N-terminal...

    Authors: Tohru Yarimizu, Mikiko Nakamura, Hisashi Hoshida and Rinji Akada

    Citation: Microbial Cell Factories 2015 14:20

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  40. Content type: Research

    The hERG potassium channel is essential for repolarization of the cardiac action potential. Due to this vital function, absence of unintended and potentially life-threatening interactions with hERG is ...

    Authors: Karen Molbaek, Peter Scharff-Poulsen, Claus Helix-Nielsen, Dan A Klaerke and Per Amstrup Pedersen

    Citation: Microbial Cell Factories 2015 14:15

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  41. Content type: Research

    The bioethanol production system used in Brazil is based on the fermentation of sucrose from sugarcane feedstock by highly adapted strains of the yeast Saccharomyces cerevisiae. Bacterial contaminants present in ...

    Authors: Osmar V Carvalho-Netto, Marcelo F Carazzolle, Luciana S Mofatto, Paulo JPL Teixeira, Melline F Noronha, Luige AL Calderón, Piotr A Mieczkowski, Juan Lucas Argueso and Gonçalo AG Pereira

    Citation: Microbial Cell Factories 2015 14:13

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