Suppression of a nonsense mutation in mammalian ce...[Nucleic Acids Res. 1985]

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1: Nucleic Acids Res. 1985 Sep 11;13(17):6265-72. Links

Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromomycin.

Burke JF, Mogg AE.

Aminoglycoside antibiotics in Escherichia coli and yeast can cause ribosomes to read through stop codons during translation. This can result in the phenotypic suppression of nonsense mutations. We show here for the first time that the aminoglycosides G-418 and paromomycin have similar effects in monkey (COS-7) cells in vivo. Suppression of an amber mutation (TAG) by aminoglycosides can restore the activity of a mutant gene transfected into COS-7 cells to almost 20% of wild type levels.

PMID: 2995924 [PubMed - indexed for MEDLINE]

PMCID: PMC321951

Introduction of UAG, UAA, and UGA nonsense mutations at a specific site in the Escherichia coli chloramphenicol acetyltransferase gene: use in measurement of amber, ochre, and opal suppression in mammalian cells. [Mol Cell Biol. 1986]
Gene selective suppression of nonsense termination using antisense agents. [Biochim Biophys Acta. 2005]
Aminoglycoside suppression at UAG, UAA and UGA codons in Escherichia coli and human tissue culture cells. [Mol Gen Genet. 1989]
ReviewEffect of structural modifications on the biological properties of aminoglycoside antibiotics containing 2-deoxystreptamine. [Adv Appl Microbiol. 1974]
ReviewPharmacologic therapy for stop mutations: how much CFTR activity is enough? [Curr Opin Pulm Med. 2004]

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Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromomycin.

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