Analysis of molecular inversion probe performance for allele copy number determination.
Wang Y,
Moorhead M,
Karlin-Neumann G,
Wang NJ,
Ireland J,
Lin S,
Chen C,
Heiser LM,
Chin K,
Esserman L,
Gray JW,
Spellman PT,
Faham M.
Affymetrix Inc, Shoreline Blvd, South San Francisco, CA 94080, USA. Yuker_Wang@affymetrix.com
We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 mug) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies.
PMID: 18028543 [PubMed - indexed for MEDLINE]
PMCID: PMC2258201