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1.

Methodology article   Open Access Highly Accessed

Rapid production of antigen-specific monoclonal antibodies from a variety of animals

Nobuyuki Kurosawa, Megumi Yoshioka, Rika Fujimoto, Fuminori Yamagishi, Masaharu Isobe BMC Biology 2012, 10:80 (28 September 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Using a fluorescent dye to label the greatly expanded endoplasmic reticulum characteristic of antibody-secreting cells, Kurosawa and colleagues demonstrate how monoclonal antibodies can be produced from a variety of immunized animals in just one week.

2.

Focus   Open Access

Programmed cell death in trypanosomatids: is it an altruistic mechanism for survival of the fittest?

Alain Debrabant, Hira Nakhasi Kinetoplastid Biology and Disease 2003, 2:7 (25 June 2003)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

3.

Review   Open Access Highly Accessed

The "impact factor" revisited

Peng Dong, Marie Loh, Adrian Mondry Biomedical Digital Libraries 2005, 2:7 (5 December 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

4.

Research   Open Access Highly Accessed

Effect of peppermint water on prevention of nipple cracks in lactating primiparous women: a randomized controlled trial

Manizheh Sayyah Melli, Mohammad Reza Rashidi, Abbas Delazar, Elaheh Madarek, Mohammad Hassan Kargar Maher, Alieh Ghasemzadeh, Kamran Sadaghat, Zohreh Tahmasebi International Breastfeeding Journal 2007, 2:7 (19 April 2007)

Abstract | Full text | PDF | PubMed

5.

Method   Open Access Highly Accessed

ZINBA integrates local covariates with DNA-seq data to identify broad and narrow regions of enrichment, even within amplified genomic regions

Naim U Rashid, Paul G Giresi, Joseph G Ibrahim, Wei Sun, Jason D Lieb Genome Biology 2011, 12:R67 (25 July 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

ZINBA is a method for analyzing peaks in next generation sequencing datasets such as ChIP-seq or DNase-seq

6.

Research article   Open Access

Markers of thrombogenesis are activated in unmedicated patients with acute psychosis: a matched case control study

Jiří Masopust, Radovan Malý, Ctirad Andrýs, Martin Vališ, Jan Bažant, Ladislav Hosák BMC Psychiatry 2011, 11:2 (3 January 2011)

Abstract | Full text | PDF | PubMed | 1 comment

7.

Review   Open Access Highly Accessed

Recent advances in asthma genetics

Jian Zhang, Peter D Paré, Andrew J Sandford Respiratory Research 2008, 9:4 (15 January 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

8.

Methodology   Open Access Highly Accessed

Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito

Li Mei, Peng Wu, Jing Ye, Guangping Gao, Lin Shao, Shaomei Huang, Yaoming Li, Xiaohong Yang, Huanchun Chen, Shengbo Cao Virology Journal 2012, 9:4 (6 January 2012)

Abstract | Full text | PDF | PubMed

9.

Review   Open Access Highly Accessed

Childhood obesity, prevalence and prevention

Mahshid Dehghan, Noori Akhtar-Danesh, Anwar T Merchant Nutrition Journal 2005, 4:24 (2 September 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 2 comments

10.

Methodology   Open Access Highly Accessed

Stool substitute transplant therapy for the eradication of Clostridium difficile infection: ‘RePOOPulating’ the gut

Elaine O Petrof, Gregory B Gloor, Stephen J Vanner, Scott J Weese, David Carter, Michelle C Daigneault, Eric M Brown, Kathleen Schroeter, Emma Allen-Vercoe Microbiome 2013, 1:3 (9 January 2013)

Abstract | Full text | PDF |  Editor’s summary

A super-probiotic synthetic stool, composed of 33 different bacterial strains, was developed and used to treat two patients with severe C. difficile infection. The probiotic bacteria could still be detected up to six months after inoculation.

11.

Research   Open Access

Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells

Hendrik Schmidt, Christoph Gelhaus, Melanie Nebendahl, Marcus Lettau, Ralph Lucius, Matthias Leippe, Dietrich Kabelitz, Ottmar Janssen Cell Communication and Signaling 2011, 9:4 (21 January 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

12.

Research article   Open Access Highly Accessed

Meat consumption and mortality - results from the European Prospective Investigation into Cancer and Nutrition

Sabine Rohrmann, Kim Overvad, H Bueno-de-Mesquita, Marianne U Jakobsen, Rikke Egeberg, Anne Tjønneland, Laura Nailler, Marie-Christine Boutron-Ruault, Françoise Clavel-Chapelon, Vittorio Krogh, Domenico Palli, Salvatore Panico, Rosario Tumino, Fulvio Ricceri, Manuela M Bergmann, Heiner Boeing, Kuanrong Li, Rudolf Kaaks, Kay-Tee Khaw, Nicholas J Wareham, Francesca L Crowe, Timothy J Key, Androniki Naska, Antonia Trichopoulou, Dimitirios Trichopoulos, Max Leenders, Petra HM Peeters, Dagrun Engeset, Christine L Parr, Guri Skeie et al. BMC Medicine 2013, 11:63 (7 March 2013)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 2 comments |  Editor’s summary

A large study finds a positive association between processed meat consumption and mortality due to cardiovascular disease and cancer, indicating that reducing the amount of processed meat in the diet could reduce mortality risk.

13.

Research   Open Access

Cancer dynamics for identical twin brothers

Ali Ghaffari, Mostafa Khazaee Theoretical Biology and Medical Modelling 2012, 9:4 (6 February 2012)

Abstract | Full text | PDF | PubMed

14.

Question and Answer   Open Access Highly Accessed

Q&A: 'Toxic' effects of sugar: should we be afraid of fructose?

Luc Tappy BMC Biology 2012, 10:42 (21 May 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Before the colonial era of sugar plantations we consumed, on average, about 15-fold less fructose than we do today. Luc Tappy explains, in question and answer format, the special features of fructose metabolism and discusses the evidence that high fructose intake has contributed to the current epidemic of obesity and metabolic disease.

15.

Research   Open Access Highly Accessed

The effect of earplugs during the night on the onset of delirium and sleep perception: a randomized controlled trial in intensive care patients

Bart Van Rompaey, Monique M Elseviers, Wim Van Drom, Veronique Fromont, Philippe G Jorens Critical Care 2012, 16:R73 (4 May 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment | F1000 Biology |  Editor’s summary

The use of earplugs in the ICU can lead to a better night's sleep for patients, lower the incidence of confusion, and delay the onset of cognitive disturbances.

16.

Research article   Open Access Highly Accessed

The weight of nations: an estimation of adult human biomass

Sarah Walpole, David Prieto-Merino, Phil Edwards, John Cleland, Gretchen Stevens, Ian Roberts BMC Public Health 2012, 12:439 (18 June 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

National food energy requirements are primarily determined by the total biomass of the population rather than the population, suggesting that increasing body mass could have the same implications for world energy requirements as an extra 473 million people on earth.

17.

Research article   Open Access Highly Accessed

Unique immunologic patterns in fibromyalgia

Frederick G Behm, Igor M Gavin, Oleksiy Karpenko, Valerie Lindgren, Sujata Gaitonde, Peter A Gashkoff, Bruce S Gillis BMC Clinical Pathology 2012, 12:25 (17 December 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Cytokine levels in stimulated blood cells taken from patients suffering from the chronic pain and fatigue syndrome fibromyalgia were significantly lower than in cells taken from healthy controls, offering a potential diagnostic test for this disease

18.

Review   Open Access Highly Accessed

Weight Science: Evaluating the Evidence for a Paradigm Shift

Linda Bacon, Lucy Aphramor Nutrition Journal 2011, 10:9 (24 January 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

19.

Opinion   Open Access Highly Accessed

Spectrum of gluten-related disorders: consensus on new nomenclature and classification

Anna Sapone, Julio C Bai, Carolina Ciacci, Jernej Dolinsek, Peter HR Green, Marios Hadjivassiliou, Katri Kaukinen, Kamran Rostami, David S Sanders, Michael Schumann, Reiner Ullrich, Danilo Villalta, Umberto Volta, Carlo Catassi, Alessio Fasano BMC Medicine 2012, 10:13 (7 February 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Fasano and colleagues propose new nomenclature and classifications within the spectrum of gluten-related disorders (GRDs), prompted by indications from the past decade that reactions to gluten may be caused by conditions other than celiac disease.

20.

Review   Open Access Highly Accessed

Physical education, school physical activity, school sports and academic performance

François Trudeau, Roy J Shephard International Journal of Behavioral Nutrition and Physical Activity 2008, 5:10 (25 February 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

21.

Minireview   Open Access Highly Accessed

Understanding the benefit of metformin use in cancer treatment

Ryan JO Dowling, Pamela J Goodwin, Vuk Stambolic BMC Medicine 2011, 9:33 (6 April 2011)

Abstract | Full text | PDF | PubMed | 1 comment |  Editor’s summary

Metformin has long been known to combat diabetes, but its mechanisms of action are now known to also provide potent anti-tumor action, reviewed here by Stambolic and colleagues.

22.

Review   Open Access Highly Accessed

ISSN exercise & sport nutrition review: research & recommendations

Richard B Kreider, Colin D Wilborn, Lem Taylor, Bill Campbell, Anthony L Almada, Rick Collins, Mathew Cooke, Conrad P Earnest, Mike Greenwood, Douglas S Kalman, Chad M Kerksick, Susan M Kleiner, Brian Leutholtz, Hector Lopez, Lonnie M Lowery, Ron Mendel, Abbie Smith, Marie Spano, Robert Wildman, Darryn S Willoughby, Tim N Ziegenfuss, Jose Antonio Journal of the International Society of Sports Nutrition 2010, 7:7 (2 February 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

23.

Research article   Open Access Highly Accessed

Managing change in the nursing handover from traditional to bedside handover – a case study from Mauritius

Hemant K Kassean, Zaheda B Jagoo BMC Nursing 2005, 4:1 (28 January 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

24.

Research   Open Access Highly Accessed

Effect of fruit restriction on glycemic control in patients with type 2 diabetes – a randomized trial

Allan S Christensen, Lone Viggers, Kjeld Hasselström, Søren Gregersen Nutrition Journal 2013, 12:29 (5 March 2013)

Abstract | Full text | PDF | PubMed

25.

Research article   Open Access Highly Accessed

Perinatal nicotine exposure induces asthma in second generation offspring

Virender K Rehan, Jie Liu, Erum Naeem, Jia Tian, Reiko Sakurai, Kenny Kwong, Omid Akbari, John S Torday BMC Medicine 2012, 10:129 (30 October 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Smoking during pregnancy induces asthma in offspring, and the effects are permanently transmitted to subsequent generations via epigenetic changes in germ cells, providing a breakthrough in studying transgenerational effects of toxins.

26.

Review   Open Access Highly Accessed

Protein timing and its effects on muscular hypertrophy and strength in individuals engaged in weight-training

Matthew Stark, Judith Lukaszuk, Aimee Prawitz, Amanda Salacinski Journal of the International Society of Sports Nutrition 2012, 9:54 (14 December 2012)

Abstract | Full text | PDF | PubMed

27.

Research   Open Access Highly Accessed

The effects of oral iron supplementation on cognition in older children and adults: a systematic review and meta-analysis

Martin Falkingham, Asmaa Abdelhamid, Peter Curtis, Susan Fairweather-Tait, Louise Dye, Lee Hooper Nutrition Journal 2010, 9:4 (25 January 2010)

Abstract | Full text | PDF | PubMed

28.

Review   Open Access Highly Accessed

Dietary protein intake and renal function

William F Martin, Lawrence E Armstrong, Nancy R Rodriguez Nutrition & Metabolism 2005, 2:25 (20 September 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

29.

Review   Open Access Highly Accessed

A macroepigenetic approach to identify factors responsible for the autism epidemic in the United States

Renee Dufault, Walter J Lukiw, Raquel Crider, Roseanne Schnoll, David Wallinga, Richard Deth Clinical Epigenetics 2012, 4:6 (10 April 2012)

Abstract | Full text | PDF | PubMed

30.

Review   Open Access Highly Accessed

Procalcitonin for diagnosis of infection and guide to antibiotic decisions: past, present and future

Philipp Schuetz, Werner Albrich, Beat Mueller BMC Medicine 2011, 9:107 (22 September 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Mueller and colleagues kick off our new thematic series on Clinical Biomarkers with a review on the utility of procalcitonin in the diagnosis of bacterial infection and decisions about antibiotic treatment in different clinical settings.

31.

Research article   Open Access Highly Accessed

A qualitative study of nursing student experiences of clinical practice

Farkhondeh Sharif, Sara Masoumi BMC Nursing 2005, 4:6 (9 November 2005)

Abstract | Full text | PDF | PubMed

32.

Research article   Open Access Highly Accessed

A stable pattern of EEG spectral coherence distinguishes children with autism from neuro-typical controls - a large case control study

Frank H Duffy, Heidelise Als BMC Medicine 2012, 10:64 (26 June 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

A large scale study assessing electroencephalography (EEG) patterns in children diagnosed with autism and matched controls identifies differences between these groups, which could be used to provide an EEG signature for autism diagnosis.

33.

Review   Open Access Highly Accessed

Nutritional therapies for mental disorders

Shaheen E Lakhan, Karen F Vieira Nutrition Journal 2008, 7:2 (21 January 2008)

Abstract | Full text | PDF | PubMed | 1 comment

34.

Commentary   Open Access Highly Accessed

Language acquisition for deaf children: Reducing the harms of zero tolerance to the use of alternative approaches

Tom Humphries, Poorna Kushalnagar, Gaurav Mathur, Donna Napoli, Carol Padden, Christian Rathmann, Scott R Smith Harm Reduction Journal 2012, 9:16 (2 April 2012)

Abstract | Full text | PDF | PubMed

35.

Debate   Open Access Highly Accessed

A theory of organizational readiness for change

Bryan J Weiner Implementation Science 2009, 4:67 (19 October 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

36.

Research   Open Access

Equol an isoflavonoid: potential for improved prostate health, in vitro and in vivo evidence

Trent D Lund, Crystal Blake, Lihong Bu, Amy N Hamaker, Edwin D Lephart Reproductive Biology and Endocrinology 2011, 9:4 (13 January 2011)

Abstract | Full text | PDF | PubMed

37.

Research article   Open Access Highly Accessed

Relationship between Organizational Culture, Leadership Behavior and Job Satisfaction

Yafang Tsai BMC Health Services Research 2011, 11:98 (14 May 2011)

Abstract | Full text | PDF | PubMed

38.

Research   Open Access Highly Accessed

A survey of energy drink consumption patterns among college students

Brenda M Malinauskas, Victor G Aeby, Reginald F Overton, Tracy Carpenter-Aeby, Kimberly Barber-Heidal Nutrition Journal 2007, 6:35 (31 October 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

39.

Review   Open Access Highly Accessed

A review of fatty acid profiles and antioxidant content in grass-fed and grain-fed beef

Cynthia A Daley, Amber Abbott, Patrick S Doyle, Glenn A Nader, Stephanie Larson Nutrition Journal 2010, 9:10 (10 March 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

40.

Review   Open Access Highly Accessed

Nutrition and cancer: A review of the evidence for an anti-cancer diet

Michael S Donaldson Nutrition Journal 2004, 3:19 (20 October 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment

41.

Review   Open Access Highly Accessed

Depression and sickness behavior are Janus-faced responses to shared inflammatory pathways

Michael Maes, Michael Berk, Lisa Goehler, Cai Song, George Anderson, Piotr Gałecki, Brian Leonard BMC Medicine 2012, 10:66 (29 June 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Michael Maes and colleagues review the protective role of inflammation in sickness behavior, in contrast to negative symptoms associated with depression, and suggest these shared immuno-inflammatory pathways can explain the phenomenological similarities between the two conditions.

42.

Research article   Open Access Highly Accessed

Factors influencing nurses' compliance with Standard Precautions in order to avoid occupational exposure to microorganisms: A focus group study

Georgios Efstathiou, Evridiki Papastavrou, Vasilios Raftopoulos, Anastasios Merkouris BMC Nursing 2011, 10:1 (21 January 2011)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Although nurses in Cyprus acknowledge the value of Standard Precautions as a means for providing protection against occupational exposure to microorganisms, several factors may contribute to their decision to not comply with the guidelines.

43.

Research article   Open Access Highly Accessed

Patient advocacy: barriers and facilitators

Reza Negarandeh, Fatemeh Oskouie, Fazlollah Ahmadi, Mansoure Nikravesh, Ingalill Hallberg BMC Nursing 2006, 5:3 (1 March 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

44.

Research article   Open Access Highly Accessed

Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice

Hiroshi Shimoda, Emi Seki, Michio Aitani BMC Complementary and Alternative Medicine 2006, 6:9 (17 March 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

45.

Correspondence   Open Access Highly Accessed

How to do a grounded theory study: a worked example of a study of dental practices

Alexandra Sbaraini, Stacy M Carter, R Evans, Anthony Blinkhorn BMC Medical Research Methodology 2011, 11:128 (9 September 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

46.

Commentary   Open Access Highly Accessed

International Society of Sports Nutrition position stand: protein and exercise

Bill Campbell, Richard B Kreider, Tim Ziegenfuss, Paul La Bounty, Mike Roberts, Darren Burke, Jamie Landis, Hector Lopez, Jose Antonio Journal of the International Society of Sports Nutrition 2007, 4:8 (26 September 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

47.

Research   Open Access Highly Accessed

An observational study of associations among maternal fluids during parturition, neonatal output, and breastfed newborn weight loss

Joy Noel-Weiss, A Kirsten Woodend, Wendy E Peterson, William Gibb, Dianne L Groll International Breastfeeding Journal 2011, 6:9 (15 August 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

The volume of intravenous fluids received by women during labour impacts newborn birth weight, meaning weight loss in the first 24 hours post delivery may not be a true measure of early breastfeeding success.

48.

Research   Open Access

The effects of spatial population dataset choice on estimates of population at risk of disease

Andrew J Tatem, Nicholas Campiz, Peter W Gething, Robert W Snow, Catherine Linard Population Health Metrics 2011, 9:4 (7 February 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

49.

Research   Open Access Highly Accessed

The importance of human resources management in health care: a global context

Stefane M Kabene, Carole Orchard, John M Howard, Mark A Soriano, Raymond Leduc Human Resources for Health 2006, 4:20 (27 July 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

50.

Review   Free Highly Accessed

Clinical review: The hospital of the future - building intelligent environments to facilitate safe and effective acute care delivery

Brian W Pickering, John M Litell, Vitaly Herasevich, Ognjen Gajic Critical Care 2012, 16:220 (26 April 2012)

Abstract | Full text | PDF | PubMed

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