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1.

Methodology article   Open Access Highly Accessed

Rapid production of antigen-specific monoclonal antibodies from a variety of animals

Nobuyuki Kurosawa, Megumi Yoshioka, Rika Fujimoto, Fuminori Yamagishi, Masaharu Isobe BMC Biology 2012, 10:80 (28 September 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Using a fluorescent dye to label the greatly expanded endoplasmic reticulum characteristic of antibody-secreting cells, Kurosawa and colleagues demonstrate how monoclonal antibodies can be produced from a variety of immunized animals in just one week.

2.

Review   Open Access Highly Accessed

Recent advances in asthma genetics

Jian Zhang, Peter D Paré, Andrew J Sandford Respiratory Research 2008, 9:4 (15 January 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

3.

Methodology   Open Access Highly Accessed

Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito

Li Mei, Peng Wu, Jing Ye, Guangping Gao, Lin Shao, Shaomei Huang, Yaoming Li, Xiaohong Yang, Huanchun Chen, Shengbo Cao Virology Journal 2012, 9:4 (6 January 2012)

Abstract | Full text | PDF | PubMed

4.

Review   Open Access Highly Accessed

Childhood obesity, prevalence and prevention

Mahshid Dehghan, Noori Akhtar-Danesh, Anwar T Merchant Nutrition Journal 2005, 4:24 (2 September 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 2 comments

5.

Research   Open Access

Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells

Hendrik Schmidt, Christoph Gelhaus, Melanie Nebendahl, Marcus Lettau, Ralph Lucius, Matthias Leippe, Dietrich Kabelitz, Ottmar Janssen Cell Communication and Signaling 2011, 9:4 (21 January 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

6.

Research   Open Access Highly Accessed

The effect of earplugs during the night on the onset of delirium and sleep perception: a randomized controlled trial in intensive care patients

Bart Van Rompaey, Monique M Elseviers, Wim Van Drom, Veronique Fromont, Philippe G Jorens Critical Care 2012, 16:R73 (4 May 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment | F1000 Biology |  Editor’s summary

The use of earplugs in the ICU can lead to a better night's sleep for patients, lower the incidence of confusion, and delay the onset of cognitive disturbances.

7.

Methodology   Open Access Highly Accessed

Stool substitute transplant therapy for the eradication of Clostridium difficile infection: ‘RePOOPulating’ the gut

Elaine O Petrof, Gregory B Gloor, Stephen J Vanner, Scott J Weese, David Carter, Michelle C Daigneault, Eric M Brown, Kathleen Schroeter, Emma Allen-Vercoe Microbiome 2013, 1:3 (9 January 2013)

Abstract | Full text | PDF |  Editor’s summary

A super-probiotic synthetic stool, composed of 33 different bacterial strains, was developed and used to treat two patients with severe C. difficile infection. The probiotic bacteria could still be detected up to six months after inoculation.

8.

Question and Answer   Open Access Highly Accessed

Q&A: 'Toxic' effects of sugar: should we be afraid of fructose?

Luc Tappy BMC Biology 2012, 10:42 (21 May 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Before the colonial era of sugar plantations we consumed, on average, about 15-fold less fructose than we do today. Luc Tappy explains, in question and answer format, the special features of fructose metabolism and discusses the evidence that high fructose intake has contributed to the current epidemic of obesity and metabolic disease.

9.

Opinion   Open Access Highly Accessed

Spectrum of gluten-related disorders: consensus on new nomenclature and classification

Anna Sapone, Julio C Bai, Carolina Ciacci, Jernej Dolinsek, Peter HR Green, Marios Hadjivassiliou, Katri Kaukinen, Kamran Rostami, David S Sanders, Michael Schumann, Reiner Ullrich, Danilo Villalta, Umberto Volta, Carlo Catassi, Alessio Fasano BMC Medicine 2012, 10:13 (7 February 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Fasano and colleagues propose new nomenclature and classifications within the spectrum of gluten-related disorders (GRDs), prompted by indications from the past decade that reactions to gluten may be caused by conditions other than celiac disease.

10.

Research   Open Access

Cancer dynamics for identical twin brothers

Ali Ghaffari, Mostafa Khazaee Theoretical Biology and Medical Modelling 2012, 9:4 (6 February 2012)

Abstract | Full text | PDF | PubMed

11.

Research article   Open Access Highly Accessed

The weight of nations: an estimation of adult human biomass

Sarah Walpole, David Prieto-Merino, Phil Edwards, John Cleland, Gretchen Stevens, Ian Roberts BMC Public Health 2012, 12:439 (18 June 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

National food energy requirements are primarily determined by the total biomass of the population rather than the population, suggesting that increasing body mass could have the same implications for world energy requirements as an extra 473 million people on earth.

12.

Minireview   Open Access Highly Accessed

Understanding the benefit of metformin use in cancer treatment

Ryan JO Dowling, Pamela J Goodwin, Vuk Stambolic BMC Medicine 2011, 9:33 (6 April 2011)

Abstract | Full text | PDF | PubMed | 1 comment |  Editor’s summary

Metformin has long been known to combat diabetes, but its mechanisms of action are now known to also provide potent anti-tumor action, reviewed here by Stambolic and colleagues.

13.

Research article   Open Access Highly Accessed

A qualitative study of nursing student experiences of clinical practice

Farkhondeh Sharif, Sara Masoumi BMC Nursing 2005, 4:6 (9 November 2005)

Abstract | Full text | PDF | PubMed

14.

Review   Open Access Highly Accessed

Physical education, school physical activity, school sports and academic performance

François Trudeau, Roy J Shephard International Journal of Behavioral Nutrition and Physical Activity 2008, 5:10 (25 February 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

15.

Review   Open Access Highly Accessed

ISSN exercise & sport nutrition review: research & recommendations

Richard B Kreider, Colin D Wilborn, Lem Taylor, Bill Campbell, Anthony L Almada, Rick Collins, Mathew Cooke, Conrad P Earnest, Mike Greenwood, Douglas S Kalman, Chad M Kerksick, Susan M Kleiner, Brian Leutholtz, Hector Lopez, Lonnie M Lowery, Ron Mendel, Abbie Smith, Marie Spano, Robert Wildman, Darryn S Willoughby, Tim N Ziegenfuss, Jose Antonio Journal of the International Society of Sports Nutrition 2010, 7:7 (2 February 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

16.

Review   Open Access Highly Accessed

Nutritional therapies for mental disorders

Shaheen E Lakhan, Karen F Vieira Nutrition Journal 2008, 7:2 (21 January 2008)

Abstract | Full text | PDF | PubMed | 1 comment

17.

Review   Open Access Highly Accessed

Weight Science: Evaluating the Evidence for a Paradigm Shift

Linda Bacon, Lucy Aphramor Nutrition Journal 2011, 10:9 (24 January 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

18.

Research   Open Access Highly Accessed

The effects of oral iron supplementation on cognition in older children and adults: a systematic review and meta-analysis

Martin Falkingham, Asmaa Abdelhamid, Peter Curtis, Susan Fairweather-Tait, Louise Dye, Lee Hooper Nutrition Journal 2010, 9:4 (25 January 2010)

Abstract | Full text | PDF | PubMed

19.

Commentary   Open Access Highly Accessed

Language acquisition for deaf children: Reducing the harms of zero tolerance to the use of alternative approaches

Tom Humphries, Poorna Kushalnagar, Gaurav Mathur, Donna Napoli, Carol Padden, Christian Rathmann, Scott R Smith Harm Reduction Journal 2012, 9:16 (2 April 2012)

Abstract | Full text | PDF | PubMed

20.

Research article   Open Access Highly Accessed

A stable pattern of EEG spectral coherence distinguishes children with autism from neuro-typical controls - a large case control study

Frank H Duffy, Heidelise Als BMC Medicine 2012, 10:64 (26 June 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

A large scale study assessing electroencephalography (EEG) patterns in children diagnosed with autism and matched controls identifies differences between these groups, which could be used to provide an EEG signature for autism diagnosis.

21.

Research article   Open Access Highly Accessed

Managing change in the nursing handover from traditional to bedside handover – a case study from Mauritius

Hemant K Kassean, Zaheda B Jagoo BMC Nursing 2005, 4:1 (28 January 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

22.

Review   Open Access Highly Accessed

A macroepigenetic approach to identify factors responsible for the autism epidemic in the United States

Renee Dufault, Walter J Lukiw, Raquel Crider, Roseanne Schnoll, David Wallinga, Richard Deth Clinical Epigenetics 2012, 4:6 (10 April 2012)

Abstract | Full text | PDF | PubMed

23.

Research   Open Access Highly Accessed

An observational study of associations among maternal fluids during parturition, neonatal output, and breastfed newborn weight loss

Joy Noel-Weiss, A Kirsten Woodend, Wendy E Peterson, William Gibb, Dianne L Groll International Breastfeeding Journal 2011, 6:9 (15 August 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

The volume of intravenous fluids received by women during labour impacts newborn birth weight, meaning weight loss in the first 24 hours post delivery may not be a true measure of early breastfeeding success.

24.

Research article   Open Access Highly Accessed

Reversal of type 1 diabetes via islet β cell regeneration following immune modulation by cord blood-derived multipotent stem cells

Yong Zhao, Zhaoshun Jiang, Tingbao Zhao, Mingliang Ye, Chengjin Hu, Zhaohui Yin, Heng Li, Ye Zhang, Yalin Diao, Yunxiang Li, Yingjian Chen, Xiaoming Sun, Mary Fisk, Randal Skidgel, Mark Holterman, Bellur Prabhakar, Theodore Mazzone BMC Medicine 2012, 10:3 (10 January 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

The safe reversal of Type 1 diabetes is possible with Stem Cell Educator therapy which uses stem cells from cord blood to re-educate a diabetic's own T cells, restarts pancreatic function, and reduces the need for insulin.

25.

Research article   Open Access Highly Accessed

Perinatal nicotine exposure induces asthma in second generation offspring

Virender K Rehan, Jie Liu, Erum Naeem, Jia Tian, Reiko Sakurai, Kenny Kwong, Omid Akbari, John S Torday BMC Medicine 2012, 10:129 (30 October 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Smoking during pregnancy induces asthma in offspring, and the effects are permanently transmitted to subsequent generations via epigenetic changes in germ cells, providing a breakthrough in studying transgenerational effects of toxins.

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