http://www.biomedcentral.com/ The latest research articles published by BioMed Central 2012-06-02T00:00:00Z This is an RSS newsfeed from BioMed Central It is intended to be used with an RSS reader. For more information about RSS newsfeeds from BioMed Central, visit http://www.biomedcentral.com/info/about/rss/ Peste des petits ruminants virus (PPRV) causes high mortality in goats and sheep and the disease has shown a greatly increased geographic distribution over the last 15 years. It is responsible for serious socioeconomic problems in some of the poorest developing countries. The ability to create recombinant PPRV would provide a useful tool for investigating the biology of the virus and the pathology of disease, as well as for developing new vaccines and diagnostic methods. Here we report the first successful rescue of recombinant PPRV from a full-length cDNA clone of the virus genome. Successful recovery of PPRV was achieved by using a RNA polymerase II promoter to drive transcription of the full-length virus antigenome. We have used this technique to construct a virus expressing a tracer protein (green fluorescent protein, GFP). The recombinant virus replicated as well as the parental virus and could stably express GFP during at least 10 passages. The newly established reverse genetics system for PPRV provides a novel method for constructing a vaccine using PPRV as a vector, and will also prove valuable for fundamental research on the biology of the virus. We found that our recombinant virus allowed more rapid and higher throughput assessment of PPRV neutralization antibody titer via the virus neutralization test (VNT) compared with the traditional method. http://www.veterinaryresearch.org/content/43/1/48 Qianqian Hu Weiye Chen Kehe Huang Michael D Baron Zhigao Bu Veterinary Research 2012, 43:48 2012-06-02T00:00:00Z ${item.identifier} /content/figures/1297-9716-43-48-toc.gif Veterinary Research 1297-9716 43 48 2012-06-02T00:00:00Z PDF No description available http://www.biomedcentral.com/1753-6561/6/S3/P24 Seung Jeong Marcia C Haigis BMC Proceedings 2012, 6:P24 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-P24-toc.gif BMC Proceedings 1753-6561 6 P24 2012-06-01T00:00:00Z XML No description available http://www.biomedcentral.com/1753-6561/6/S3/O15 Jason W Locasale Alexandra Grassian Rameen Beroukhim Matthew Meyerson Gerhard Wagner John M Asara Joan S Brugge Matthew G Vander Heiden Lewis C Cantley BMC Proceedings 2012, 6:O15 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-O15-toc.gif BMC Proceedings 1753-6561 6 O15 2012-06-01T00:00:00Z XML No description available http://www.biomedcentral.com/1753-6561/6/S3/O19 Christopher B Newgard BMC Proceedings 2012, 6:O19 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-O19-toc.gif BMC Proceedings 1753-6561 6 O19 2012-06-01T00:00:00Z XML No description available ${item.link} BMC Proceedings 2012, 6:P20 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-P20-toc.gif BMC Proceedings 1753-6561 6 P20 2012-06-01T00:00:00Z XML No description available http://www.biomedcentral.com/1753-6561/6/S3/O12 Rudolf Kaaks BMC Proceedings 2012, 6:O12 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-O12-toc.gif BMC Proceedings 1753-6561 6 O12 2012-06-01T00:00:00Z XML No description available ${item.link} BMC Proceedings 2012, 6:O4 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-O4-toc.gif BMC Proceedings 1753-6561 6 O4 2012-06-01T00:00:00Z XML No description available http://www.biomedcentral.com/1753-6561/6/S3/P26 Gerald L Klein Eric M Weaver BMC Proceedings 2012, 6:P26 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-P26-toc.gif BMC Proceedings 1753-6561 6 P26 2012-06-01T00:00:00Z XML No description available http://www.biomedcentral.com/1753-6561/6/S3/P27 Silvina M Kroetz Jagan Srinivasan Jonathan Yaghoobian Paul W Sternberg Ray L Hong BMC Proceedings 2012, 6:P27 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-P27-toc.gif BMC Proceedings 1753-6561 6 P27 2012-06-01T00:00:00Z XML No description available http://www.biomedcentral.com/1753-6561/6/S3/O16 Steven A Kliewer David J Mangelsdorf BMC Proceedings 2012, 6:O16 2012-06-01T00:00:00Z ${item.identifier} /content/figures/1753-6561-6-S3-O16-toc.gif BMC Proceedings 1753-6561 6 O16 2012-06-01T00:00:00Z XML