Absence of Wolbachia endobacteria in the human parasitic nematode Dracunculus medinensis and two related Dracunculus species infecting wildlife
Parasites & Vectors 2014, 7:140 (31 March 2014)
Most filarial nematodes that infect humans harbour essential Wolbachia endobacteria - a proven target for antibiotic control of filarial infections. We demonstrate that the closely related guinea worm, Dracunculus medinensis, is not colonized by Wolbachia endosymbionts. Image: Extraction of guinea worm, Dracunculus medinensis. (courtesy The Carter Center.)
Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase – engineering a thermostable ATP independent enzyme
BMC Molecular Biology 2012, 13:24 (18 July 2012)
Mutation of a single amino acid residue in the Methanobacterium thermoautotrophicum RNA ligase results in ATP-independent ligase activity at high temperatures, suggesting that this RNA ligase could be a useful tool for RNA ligation experiments.
SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm
Microbial Cell Factories 2012, 11:56 (8 May 2012)
Efficient in vitro RNA interference and immunofluorescence-based phenotype analysis in a human parasitic nematode, Brugia malayi
Parasites & Vectors 2012, 5:16 (13 January 2012)
This manuscript demonstrates the efficient use of heterogeneous short interfering RNA mixes for performing RNAi in the parasitic nematode Brugia malayi and immunofluorescence-based analysis of cellular phenotypes. Image: Defective 2 cell embryo of Brugia malayi following RNAi targeting the polarity-determining gene, par-1.
T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis
BMC Biotechnology 2011, 11:72 (1 July 2011)
Controlled expression of functional miR-122 with a ligand inducible expression system
BMC Biotechnology 2010, 10:76 (20 October 2010)
A new expression system relying on modified nuclear receptors and a synthetic ligand allows the construction of stable cell lines where the expression of miRNA or siRNA can be tightly and reversibly controlled.
Open source tool for prediction of genome wide protein-protein interaction network based on ortholog information
Source Code for Biology and Medicine 2010, 5:8 (4 August 2010)
Cloning of NruI and Sbo13I restriction and modification sstems in E. coli and amino acid sequence comparison of M.NruI and M.Sbo13I with other amino-methyltransferases
BMC Research Notes 2010, 3:139 (24 May 2010)
DNA damage in preserved specimens and tissue samples: a molecular assessment
Frontiers in Zoology 2008, 5:18 (23 October 2008)
A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based
BMC Biotechnology 2008, 8:58 (29 July 2008)