BioMed Central | Members | New England Biolabs

1.

Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase – engineering a thermostable ATP independent enzyme

Alexander M Zhelkovsky, Larry A McReynolds BMC Molecular Biology 2012, 13:24 (18 July 2012)

Abstract | Full text | PDF | PubMed | Editor’s summary

Mutation of a single amino acid residue in the Methanobacterium thermoautotrophicum RNA ligase results in ATP-independent ligase activity at high temperatures, suggesting that this RNA ligase could be a useful tool for RNA ligation experiments.

2.

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

Julie Lobstein, Charlie A Emrich, Chris Jeans, Melinda Faulkner, Paul Riggs, Mehmet Berkmen Microbial Cell Factories 2012, 11:56 (8 May 2012)

3.

Efficient in vitro RNA interference and immunofluorescence-based phenotype analysis in a human parasitic nematode, Brugia malayi

Frédéric Landmann, Jeremy M Foster, Barton E Slatko, William Sullivan Parasites & Vectors 2012, 5:16 (13 January 2012)

This manuscript demonstrates the efficient use of heterogeneous short interfering RNA mixes for performing RNAi in the parasitic nematode Brugia malayi and immunofluorescence-based analysis of cellular phenotypes. Image: Defective 2 cell embryo of Brugia malayi following RNAi targeting the polarity-determining gene, par-1.

4.

T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis

Sebastien Viollet, Ryan T Fuchs, Daniela B Munafo, Fanglei Zhuang, Gregory B Robb BMC Biotechnology 2011, 11:72 (1 July 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

5.

Controlled expression of functional miR-122 with a ligand inducible expression system

Cathy M Shea, George Tzertzinis BMC Biotechnology 2010, 10:76 (20 October 2010)

Abstract | Full text | PDF | PubMed | Editor’s summary

A new expression system relying on modified nuclear receptors and a synthetic ligand allows the construction of stable cell lines where the expression of miRNA or siRNA can be tightly and reversibly controlled.

6.

Open source tool for prediction of genome wide protein-protein interaction network based on ortholog information

Chandra Sekhar Pedamallu, Janos Posfai Source Code for Biology and Medicine 2010, 5:8 (4 August 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

7.

Cloning of NruI and Sbo13I restriction and modification sstems in E. coli and amino acid sequence comparison of M.NruI and M.Sbo13I with other amino-methyltransferases

Zhenyu Zhu, Chandra Pedamallu, Alexey Fomenkov, Jack Benner, Shuang-yong Xu BMC Research Notes 2010, 3:139 (24 May 2010)

Abstract | Full text | PDF | PubMed

8.

DNA damage in preserved specimens and tissue samples: a molecular assessment

Juergen Zimmermann, Mehrdad Hajibabaei, David C Blackburn, James Hanken, Elizabeth Cantin, Janos Posfai, Thomas C Evans Frontiers in Zoology 2008, 5:18 (23 October 2008)

Abstract | Full text | PDF | PubMed

9.

A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based

Jason R Hillebrecht, Shaorong Chong BMC Biotechnology 2008, 8:58 (29 July 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

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	1.	Research article *Structure-function analysis of Methanobacterium thermoautotrophicum* RNA ligase – engineering a thermostable ATP independent enzyme** Alexander M Zhelkovsky, Larry A McReynolds BMC Molecular Biology 2012, 13:24 (18 July 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Mutation of a single amino acid residue in the Methanobacterium thermoautotrophicum RNA ligase results in ATP-independent ligase activity at high temperatures, suggesting that this RNA ligase could be a useful tool for RNA ligation experiments.
	2.	Research *SHuffle, a novel Escherichia coli* protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm** Julie Lobstein, Charlie A Emrich, Chris Jeans, Melinda Faulkner, Paul Riggs, Mehmet Berkmen Microbial Cell Factories 2012, 11:56 (8 May 2012) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| f1000
	3.	Research Efficient in vitro RNA interference and immunofluorescence-based phenotype analysis in a human parasitic nematode, Brugia malayi Frédéric Landmann, Jeremy M Foster, Barton E Slatko, William Sullivan Parasites & Vectors 2012, 5:16 (13 January 2012) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary This manuscript demonstrates the efficient use of heterogeneous short interfering RNA mixes for performing RNAi in the parasitic nematode Brugia malayi and immunofluorescence-based analysis of cellular phenotypes. Image: Defective 2 cell embryo of Brugia malayi following RNAi targeting the polarity-determining gene, par-1.
	4.	Research article T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis Sebastien Viollet, Ryan T Fuchs, Daniela B Munafo, Fanglei Zhuang, Gregory B Robb BMC Biotechnology 2011, 11:72 (1 July 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	5.	Research article Controlled expression of functional miR-122 with a ligand inducible expression system Cathy M Shea, George Tzertzinis BMC Biotechnology 2010, 10:76 (20 October 2010) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A new expression system relying on modified nuclear receptors and a synthetic ligand allows the construction of stable cell lines where the expression of miRNA or siRNA can be tightly and reversibly controlled.
	6.	Software review Open source tool for prediction of genome wide protein-protein interaction network based on ortholog information Chandra Sekhar Pedamallu, Janos Posfai Source Code for Biology and Medicine 2010, 5:8 (4 August 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	7.	Research article *Cloning of NruI and Sbo13I restriction and modification sstems in E. coli* and amino acid sequence comparison of M.NruI and M.Sbo13I with other amino-methyltransferases** Zhenyu Zhu, Chandra Pedamallu, Alexey Fomenkov, Jack Benner, Shuang-yong Xu BMC Research Notes 2010, 3:139 (24 May 2010) Abstract \| Full text \| PDF \| PubMed
	8.	Research DNA damage in preserved specimens and tissue samples: a molecular assessment Juergen Zimmermann, Mehrdad Hajibabaei, David C Blackburn, James Hanken, Elizabeth Cantin, Janos Posfai, Thomas C Evans Frontiers in Zoology 2008, 5:18 (23 October 2008) Abstract \| Full text \| PDF \| PubMed
	9.	Research article *A comparative study of protein synthesis in in vitro* systems: from the prokaryotic reconstituted to the eukaryotic extract-based** Jason R Hillebrecht, Shaorong Chong BMC Biotechnology 2008, 8:58 (29 July 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central