Fanconi anemia
Fanconi anemia (FA) is a rare autosomal or X-linked recessive disease characterized by chromosomal instability and cancer susceptibility. All FA complementation groups (discussed below) except FA-B are inherited autosomally 12. FA prevalence is estimated to be 1–5 per million and the frequency of heterozygous carriers to be 1 in 300 34. Notwithstanding the small number of FA patients, this disease constitutes a dynamic research area because the FA pathway plays a crucial role in preventing genomic instability and provides an attractive model for understanding the interplay between DNA repair and ubiquitin biology in tumorigenesis and cancer therapy (reviewed in 56789101112131415).
The clinical course and the treatment of FA have been extensively reviewed elsewhere 3416. Clinically, FA is characterized by childhood onset aplastic anemia, increased cancer/leukemia susceptibility and developmental defects. Typically, FA patients develop bone marrow failure leading to aplastic anemia during the first decade of life and at least 20% develop malignancies. Most commonly, these include acute myelogenous leukemia and myelodysplastic syndrome, but also head and neck squamous cell carcinoma, gynecological squamous cell carcinoma, esophageal carcinoma, and liver, brain, skin and renal tumors 171819. FA subtypes FA-D1 and FA-N are associated with increased predisposition to medulloblastoma, Wilms' tumor and acute leukemia in early childhood, and are clinically different from the other FA subtypes 2021222324. Common developmental defects observed in FA are short stature, developmental disability and abnormalities of the skin, upper extremities, head, eyes, kidneys and ears 3. In addition, male FA patients often present abnormal gonads 3.
FA cells are hypersensitive to treatment with DNA crosslinking agents such as cisplatin, mitomycin C (MMC), melphalan and diepoxybutane 25, leading to increased chromosome breakage 2526.
The Fanconi anemia pathway
Proteins involved in the Fanconi anemia pathway
FA can be divided into at least thirteen complementation groups (FA-A, -B, -C, -D1, -D2, -E, -F, -G, -I, -J, -L, -M and -N), of which the responsible genes for all groups except FA-I have been identified (summarized in Table 1) 6232427282930. All FA proteins are required for cellular resistance to DNA crosslinking agents 6 and are considered to cooperate in a common pathway (the FA pathway) that regulates the sensing, signaling and/or repair of interstrand DNA crosslinks (Figure 1). FA proteins are closely related to the breast/ovarian cancer susceptibility genes products BRCA1 and BRCA2, and to their partner proteins, as described below. FANCD1 (mutated in FA-D1) is identical to BRCA231 and the most recently identified FA gene, FANCN, is in fact PALB2 (
artner nd ocalizer of RCA2) 2324, a crucial regulator of the BRCA2 protein 32. Additionally, FANCJ is identical to BACH1/BRIP1293033, a DNA helicase that interacts directly with BRCA1. Furthermore, FANCD2, FANCD1/BRCA2, FANCN/PALB2 and BRCA1 colocalize in nuclear foci at the site of DNA damage 323435 and BRCA1 itself is required for efficient nuclear foci formation of FANCD2 3436. In light of these interplays between FA and BRCA proteins, the FA pathway is also called the “FA-BRCA pathway” 5 or “FA-BRCA network” 11.
<p>Table 1</p>
The responsible genes for Fanconi anemia
Subtype
Responsible gene
Protein (kDa)
Requirement for FANCD2 monoubiquitylation
Function of the protein, etc.
Homologs in
Yeast
Plant
Fly
Worm
Fish
Amphibian
Human, mouse, chicken
S. cerevisiae
A. thaliana
D. melanogaster
C. elegans
D. rerio
X. laevis
A
FANCA
163
+
FA core complex
+ [123]
+ [124]
+
B
FANCB
95
+
FA core complex
+ [123]
+ [124]
+
C
FANCC
63
+
FA core complex
+ [123]
+ [124]
+
D1
FANCD1/BRCA2
380
-
RAD51 recruitment
+ [135]
+ [123]
+ [124]
+
D2
FANCD2
155,162
+
monoubiquitylated protein
+ [133]
+ [27,125]
+ [126]
+ [123,127]
+ [124]
+
E
FANCE
60
+
FA core complex
+ [123]
+ [124]
+
F
FANCF
42
+
FA core complex
+ [123]
+ [136]
+
G
FANCG/XRCC9
68
+
FA core complex
+ [123]
+ [124]
+
I
not identified
?
+
?
J
FANCJ/BACH1/BRIP1
130
-
5′→3′ DNA helicase/ATPase
+ [132]
+ [126]
+
L
FANCL/PHF9/POG
43
+
FA core complex, E3 ubiquitin ligase
+ [134]
+ [27,125]
+ [123]
+ [124]
+
M
FANCM/Hef
250
+
FA core complex, ATPase/translocase, DNA helicase motifs
+ [27]
+ [125]
+ [126]
+
N
FANCN/PALB2
130
-
regulation of BRCA2 localization
+
+
+
(Adapted with modifications from “The molecular pathogenesis of Fanconi anemia: recent progress” Taniguchi T and D'Andrea AD. Blood, Jun 2006; 107: 4223–4233. [6])
FANC protein homologs shown in the table are limited to the ones reported in the literature [27,123–127,132–136].
<p>Figure 1</p>
Current model of the Fanconi anemia pathway
Current model of the Fanconi anemia pathway. (Adapted with modifications from “The molecular pathogenesis of Fanconi anemia: recent progress” Taniguchi T and D'Andrea AD. Blood, Jun 2006; 107: 4223 – 4233. 6) Eight FA proteins (FANC-A, -B, -C, -E, -F, -G, -L and -M), a FANCM-interacting protein called FAAP24 and an unidentified factor (FAAP100) form a nuclear complex (the FA core complex) with E3 ubiquitin ligase activity. FANCL is the catalytic subunit of the complex and directly interacts with the E2 ubiquitin conjugating enzyme UBE2T through its PHD/RING domain. UBE2T can be inactivated by auto-monoubiquitylation on lysine 91 (K91), but is suggested to form a thioester intermediate with ubiquitin through it's catalytic cysteine 86 (C86) when taking part in the ubiquitylation of FANCD2 (see below). The FA core complex, BLM, RPA and topoisomerase IIIα form a larger super-complex called BRAFT. Note that for diagrammatic purposes the FA core complex is shown here interacting with the FAAP100 subunit, but in reality the quaternary structure of the BRAFT complex is unknown. In response to exogenous DNA damage, or during normal S phase progression, the FANCD2 protein is monoubiquitylated on lysine 561 (K561) in an FA core complex- and UBE2T-dependent manner. DNA damage-induced monoubiquitylation of FANCD2 also requires ATR and RPA. Monoubiquitylation of FANCD2 targets the protein into nuclear foci where it co-localizes with BRCA1, FANCD1/BRCA2, FANCN/PALB2, RAD51, FANCJ/BRIP1 and other proteins. At least some components of the FA core complex (FANCC, FANCE and FANCG) also form nuclear foci. All of these factors are required for cellular resistance to DNA crosslinking agents. Monoubiquitylation of PCNA on lysine 164 (K164) requires RAD6 as an E2 and RAD18 as an E3, but not the FA core complex. In turn, modification of PCNA causes recruitment of translesion synthesis (TLS) DNA polymerases at the site of stalled replication forks. USP1 deubiquitylates both PCNA and FANCD2, negatively regulating both the Fanconi anemia pathway and monoubiquitylation of PCNA.
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The FA core complex and monoubiquitylation of FANCD2
Ubiquitin plays a crucial role in the regulation of the FA pathway. Eight FA proteins (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL and FANCM), a newly identified FANCM-interacting protein called FAAP24 (
NCA-ssociated olypeptide) 137 and an unidentified factor called FAAP100 form a nuclear protein complex (the FA core complex) required for monoubiquitylation of FANCD2 on lysine 561 27343738. One of the components of the FA core complex, FANCL, has a PHD (plant homeodomain) finger/RING finger domain exhibiting auto-ubiquitin ligase activity in vitro38394041. FANCL associates through its PHD/RING finger domain with UBE2T, a ubiquitin conjugating enzyme (E2), which is also required for in vivo FANCD2 monoubiquitylation 42. Taken together, the FA core complex is assumed to constitute a multi-subunit E3 ubiquitin ligase complex for FANCD2, in which FANCL is the catalytic E3 ubiquitin ligase subunit. The catalytic activity of this complex appears to be regulated through the inhibitory auto-monoubiquitylation of UBE2T, stimulated in the presence of FANCL 42. However, as the direct monoubiquitylation of FANCD2 by the FA core complex has not been reconstituted so far in vitro, the existence of other E2 and E3 enzymes responsible for FANCD2 monoubiquitylation (and somehow controlled by the FA core complex, the E2 activity of UBE2T and the E3 activity of FANCL) cannot be ruled out formally.
Even though a direct DNA binding activity has been demonstrated for unmodified FANCD2 in vitro43, monoubiquitylation of the protein is required for its translocation to chromatin in vivo and nuclear foci formation at the site of DNA damage, as well as for cellular resistance to DNA crosslinking agents 3444. FANCD2 monoubiquitylation and nuclear foci formation occur in response to DNA damaging agents (ionizing radiation, UV light irradiation, DNA crosslinking agents, hydroxyurea, etc.) and during S phase of the cell cycle even in the absence of exogenous DNA damage 3445. A DNA damage-activated signaling kinase, ATR, and a single-strand DNA binding protein complex, RPA, are required for DNA damage-inducible monoubiquitylation and foci formation of FANCD2, indicating an upstream role for these factors in the activation of the FA pathway 46. The exact signal and activation cascade required for FANCD2 monoubiquitylation, however, remain elusive. The existence of a specific FANCD2 receptor responsible for recruitment of the monoubiquitylated protein to chromatin has also been proposed 44, but not demonstrated to date.
Roles for the FA core complex in DNA enzymatic processing
The FA core complex is not simply the ubiquitin ligase complex for FANCD2. In addition to its requirement for FANCD2 monoubiquitylation, the FA core complex is required for the translocation into chromatin of monoubiquitylated FANCD2, and for cellular resistance to interstrand DNA crosslinks even if FANCD2 is localized in chromatin, as elegantly demonstrated in chicken DT40 cells using FANCD2–monoubiquitin and FANCD2–histone H2B chimeric proteins 47. In addition, one component of the FA core complex, FANCM, harbors DNA helicase motifs, a degenerate nuclease motif and in vitro DNA-stimulated ATPase and translocase activities 27. The newly identified FAAP24 protein, in complex with FANCM, has been shown to preferentially bind to ssDNA and branched DNA structures 137. Speculatively, FANCM DNA translocase activity could play an important role in displacing the FA core complex along the DNA, allowing DNA damage recognition, or FAAP24 specificity for ssDNA structures may target FANCM and the FA core complex to abnormal, branched DNA structures. In summary, the FA core complex itself can interact with DNA and displays some important functions outside of FANCD2 monoubiquitylation.
Furthermore, the FA core complex forms a larger complex with BLM, RPA and topoisomerase IIIα called BRAFT (for
LM, P, A, and opoisomerase IIIα). Although the functional relevance of BRAFT is not clear, it harbors a DNA unwinding activity potentially relevant for DNA repair 37.
Negative regulation of the Fanconi anemia pathway by FANCD2 deubiquitylation
FANCD2 monoubiquitylation is a regulated and reversible process. USP1, a deubiquitylating enzyme, removes ubiquitin from monoubiquitylated FANCD2, therefore negatively regulating the FA pathway 48. Interestingly, USP1 also deubiquitylates monoubiquitylated PCNA (
roliferating ell uclear ntigen), a DNA polymerase processivity factor 49. PCNA monoubiquitylation leads to the switch from a replicative polymerase to a translesion synthesis (TLS) polymerase at the site of stalled replication forks 50 and participates in the bypass of DNA lesion. Monoubiquitylation of PCNA is dependent on RAD6 (an E2) and RAD18 (an E3) 51, but is independent of the FA core complex. Therefore, these two pathways (the FA pathway and TLS activation through PCNA monoubiquitylation) have different activation mechanisms, but share a common shutoff mechanism.
The Fanconi anemia pathway and DNA repair
Interestingly, some TLS polymerases are also implicated in cellular resistance to interstrand DNA crosslinks 52. For example, mutations in TLS polymerase-encoding REV3 or REV1 have been shown to be epistatic with FANCC mutations for crosslinker hypersensitivity in chicken DT40 cells 53. Furthermore, REV1 and FANCD2 colocalize in nuclear foci 53, which could suggest a possible interaction between them. The possible interplay of FA proteins and TLS polymerases in a common pathway regulating the bypass of DNA damage, however, remains to be clarified.
One of the most important issues to clarify concerning the role of the FA pathway in DNA repair is how FA proteins (the FA core complex, FANCD2, FANCD1/BRCA2, FANCN/PALB2, FANCJ/BRIP1/BACH1) and other factors (RAD51, BRCA1, BLM, TLS polymerases etc.) cooperate to sense, signal and repair DNA crosslinks.
In nuclear foci, FANCD2 colocalizes with factors known to be involved in DNA repair and/or DNA damage response, such as BRCA1, FANCD1/BRCA2, FANCN /PALB2, RAD51, BLM, RPA and ATR 323435364546. FANCD2 also partially colocalizes with FANCJ/BRIP1/BACH1 33 and NBS1, part of the MRE11–RAD50–NBS1 complex implicated in the recognition and early signalization of damaged DNA 54. Among the subunits of the FA core complex, at least FANCC and FANCE are reported to colocalize with FANCD2 in nuclear foci 4755, and FANCG with BRCA2 and RAD51 56. All these factors are required for cellular resistance to interstrand DNA crosslinking agents.
Among FA proteins, FANCD1/BRCA2 and FANCJ/BRIP1/BACH1 are neither part of the FA core complex nor required for FANCD2 monoubiquitylation or foci formation 2831. They may work downstream of FANCD2 monoubiquitylation and/or in another pathway. Interestingly, these proteins have been strongly linked to DNA double-strand breaks repair by homologous recombination (HR) 3357. Specifically, FANCD1/BRCA2 regulates HR by controlling the activity of RAD51, the eukaryotic homolog of bacterial RecA 5758. FANCD1/BRCA2 stability and localization in nuclear structures (chromatin and nuclear matrix) is in turn regulated by FANCN/PALB2 32. Whether FANCD1/BRCA2, FANCN/PALB2 and RAD51 act downstream or independently of monoubiquitylated FANCD2 and other FA proteins remains unclear. Evidence in favor of a regulatory role for the FA pathway on FANCD1/BRCA2 and RAD51 function comes from reports that monoubiquitylated FANCD2 is required for both the loading of FANCD1/BRCA2 onto damaged chromatin and the increase in FANCD1/BRCA2 and RAD51 nuclear foci in response to DNA damage 35. However, the requirement for the FA core complex and FANCD2 in efficient RAD51 foci formation in response to DNA damage 355960, as well as the efficiency of HR in FA cells (reviewed in reference 6), remain controversial. FANCD1/BRCA2- and FANCN/PALB2-deficient cells have a clear defect in RAD51 foci formation and HR efficiency 233257616263. In other FA cells, at least mild defects in HR have been documented 33536264656667, although other reports show normal HR efficiency in these cells 686970.
FANCJ/BRIP1/BACH1 has DNA-dependent ATPase activity and 5′–3′ DNA helicase activity, and directly binds to the phospho-protein binding BRCT (
CA1 arboxyl-erminal domain 7273) of BRCA1 71. How FANCJ/BRIP1/BACH1 interacts with other FA proteins has not been reported, except for partial colocalization with FANCD2 in nuclear foci 33. Though RAD51 foci formation is normal in FA-J cells (deficient in FANCJ/BRIP1/BACH1) 63, HR efficiency appears to be affected 33.
Taken together, these evidences suggest that FANCD2 plays a role in regulating DNA repair (especially of interstrand DNA crosslinks) in chromatin in cooperation with BRCA1, FANCD1/BRCA2, FANCJ/BRIP1/BACH1, the FA core complex and other factors, but the precise mechanism by which this occurs remains unknown. The highly regulated process of FANCD2 monoubiquitylation could play a crucial role in orienting the repair of DNA damage to HR and/or in attracting TLS polymerases to sites of DNA damage.
Other roles for the Fanconi anemia proteins outside of DNA repair
Several FA proteins function outside of DNA crosslink repair. For example, the phosphorylation of FANCD2 at serine 222 by ATM kinase in response to ionizing radiation is required for the establishment of IR-induced intra-S phase checkpoint, but is not required for resistance to DNA crosslinking agents 74. Additionally, FANCC (localized in both the cytoplasm and nucleus) is implicated in several cytoplasmic functions, such as JAK/STAT and apoptotic signaling 757677787980.
Alterations in the Fanconi anemia pathway in human cancer in the general (non-FA) population
FA patients have an increased risk of developing leukemia and solid tumors, but alterations in the FA pathway have also been reported in a wide variety of human cancers in the general (non-FA) population 8182838485868788899091929394 (reviewed in references 610). Abnormalities in BRCA1 and BRCA2 in cancer have been reviewed elsewhere 95. Methylation of FANCF, leading to its decreased expression, has been reported in ovarian cancer 8182, breast cancer 84, non-small cell lung cancer 85, cervical cancer 86, testicular cancer 87, head and neck squamous cell carcinoma 85, and granulosa cell tumors of the ovary 83. Inherited and somatic mutations of FANCC and FANCG have been detected in a subset of young onset pancreatic cancer 8996. Additionally, inherited mono-allelic mutations in BACH1/BRIP1/FANCJ and FANCN/PALB2 have been recently implicated in breast cancer predisposition (familial breast cancer) 972324, suggesting that these genes may be the low penetrance breast/ovarian cancer susceptibility genes researchers have been looking for to explain the non-BRCA1/non-BRCA2 cases. Because the integrity of the FA pathway is crucial for cellular resistance to interstrand DNA crosslinking agents (cisplatin, MMC, melphalan, etc.), tumors with defects in the pathway may be hypersensitive to these agents. In fact, in some cancer cell lines (human ovarian cancer cell lines (TOV-21G and 2008) 81, human myeloma cell lines (8226 and U266) 98 and a human pancreatic cancer cell line (PL11) 99), the integrity of the FA pathway is a determinant of cisplatin (or melphalan) resistance in vitro or in vivo (mouse xenograft model) 819899100.