GOptions Device=Win; Data TR; Infile 'X:\...' ExpandTabs; Input Con Ct Class; Run; Title1 'Real-time PCR data Analysis'; Title2 'Correlation model for slope estimation'; Data TR; set TR; lcon=log(Con)/log(2); Proc print data=TR; run; Proc mixed Data = TR; By Class; Model Ct = lcon /Cl Alpha=0.05; Run; Goptions reset=global gunit=pct border cback=white colors=(black blue green red) ftitle=swissb ftext=swiss htitle=6 htext=3; Symbol1 color=green width=2 value=triangle height=3; Symbol2 color=blue width=2 value=dot height=3; Symbol3 color=red width=2 value=square height=3; Symbol4 color=black width=2 value=circle height=3; Title 'Data Quality Evaluation for Real-time PCR'; proc gplot data=TR; plot Ct*lcon=Class; run; quit; ÿÿ