A number of factors that relay the local activity of the plant hormone auxin in plant development have been identified 456789. They include factors that actively regulate auxin distribution, such as PIN-FORMED (PIN) proteins, as well as transcriptional regulators that convert cellular auxin concentration into gene-expression responses. PIN-dependent auxin transport is essential for embryonic axis formation 6, as is cellular auxin signaling, which is intertwined with auxin transport through feedback loops 101112. Two key mutants in embryonic auxin signaling, bodenlos (bdl) and monopteros (mp), fail to form an embryonic root meristem and therefore yield rootless seedlings 1314. BDL encodes a transcriptional corepressor of the AUX/IAA family that inhibits the activation potential of auxin-response factor (ARF) transcription factors, such as the MP protein, by direct protein-protein interaction 7815. Several lines of investigation have demonstrated that AUX/IAA proteins are degraded upon auxin-mediated interaction with auxin receptors, resulting in the release of ARFs to activate downstream targets of auxin signaling 1617. The dominant bdl mutant carries a mutation that desensitizes BDL protein to auxin-induced degradation. Similarly to the loss of MP activity in recessive mp mutants, this results in severely impaired auxin-dependent embryonic gene expression.

The tpl mutation is a rare temperature-sensitive mutation that changes apical cell fate. In the first examinations of a possible role for TPL in the auxin pathway by Long et al. 2, double mutants were generated between tpl and mp. Apically, these largely resembled tpl single mutants, whereas basally they resembled mp, although the reduced hypocotyl and vascular differentiation of mp was partially suppressed by tpl (Figure 1a). When the synthetic DR5 reporter construct, which monitors ARF activity and thereby auxin concentration, was introduced into tpl embryos, it rarely displayed strong ectopic apical expression 2. Altogether, these findings led Long et al. to suggest that disturbance in the auxin pathway could not be responsible for the tpl phenotype. However, further experiments with the TPL protein by the same laboratory 3 reveal that this assumption was premature.

Molecular cloning of TPL indicated that the TPL protein resembles known transcriptional corepressors 18. Consistent with this idea, TPL is localized to the nucleus and mutations in a histone acetyltransferase, a known transcriptional coactivator, suppress tpl phenotypes. The tpl mutation acts as a dominant negative, probably interfering with the activity of other TPL-related proteins 18. The quintuple knockout of the whole TPL-related gene family confirmed this notion, as it phenocopies the original tpl semi-dominant allele 18. TPL is initially expressed throughout the embryo and then restricted to the incipient vasculature, resembling the expression patterns of MP and BDL 18.

Szemenyei et al. 3 have now found that TPL interacts directly with BDL and indirectly, by BDL bridging, with MP in planta, suggesting that these proteins can exist in a ternary complex (Figure 1b). They determined the biological significance of these interactions by generating tpl bdl double mutants. Strikingly, these double mutants, unlike bdl single mutants, formed hypocotyls, roots and cotyledon vasculature, suggesting that tpl can suppress both basal and apical bdl patterning defects 3. This suppression can be traced back to embryogenesis, demonstrating that tpl bdl double-mutant embryos form normal basal structures with restored auxin responsiveness as monitored by DR5. Conversely, while another, loss-of-function, allele of bdl does not display visible phenotypes, it enhances the frequency of severe tpl phenotypes at the permissive temperature (24°C) in the respective double mutant with tpl.

To corroborate these findings, Szemenyei et al. 3 carried out a series of innovative in planta experiments, which showed that TPL affects transcriptional repression by BDL. Moreover, expression of a chimeric protein consisting of the MP-interaction domain of BDL and the repression domain of TPL triggered bdl and mp phenotypes. Importantly, this chimeric protein was missing the BDL domain required for its auxin-mediated degradation, as well as the EAR motif of BDL, which mediates transcriptional repression of ARFs 15 and interaction with TPL (Figure 1b). Thus, the EAR motif of AUX/IAA proteins appears to repress transcription by recruiting TPL-family proteins and bringing them in close vicinity to ARFs.

How can the findings about the molecular interplay of these diverse players be reconciled with the various mutant phenotypes? A parsimonious explanation would be that they reflect region-specific variations in ARF activity. For instance, in the wild-type embryo, the polar auxin-transport machinery concentrates auxin at the basal pole of the embryo, leading to high ARF (here mainly MP) activity and thus root formation 6. In contrast, in the apical regions of the embryo auxin concentration, and presumably ARF activity, is lower, resulting in formation of a shoot rather than a root. In tpl mutants, the maximal interference with TPL-like activity at restrictive temperatures might remove the repression of ARF activity to a level that overrides the normally insufficient apical auxin concentration, resulting in formation of root instead of shoot structures.

A caveat to this idea is the weak or absent apical DR5 reporter expression in early tpl embryos 2. However, this could reflect the temporal delay associated with reprogramming of the shoot or the fact that DR5 is an artificial reporter of global auxin-induced transcription and may not always faithfully monitor activity of auxin target subsets. Global interference with the auxin pathway by the mutant protein tpl might not be necessary to initiate root formation. Rather, above-threshold expression of a few ARF-controlled master regulators of root formation, such as the PLETHORA genes 19, might suffice.

In tpl mp double mutants, the dominant-negative effects of the tpl mutation are limited by the absence of MP, particularly in the basal embryo, where this ARF is the limiting factor for root formation 8. In the apical embryo, however, where other ARFs act redundantly with MP 20, tpl can still exert an effect, which explains the partial restoration of mp hypocotyl and cotyledon vasculature defects and the occurrence of apical roots in tpl mp double mutants.

So what about the tpl single-mutant phenotypes at more permissive temperatures? In these conditions, apical roots are rarely observed. Rather, the tpl seedlings increasingly display fused cotyledons 2. This temperature-sensitive shift in tpl phenotypes could simply reflect a temperature-dependent decrease in auxin levels 21 and thus less stringent requirement for TPL activity. Therefore, the phenotypes of tpl at more permissive temperatures might reflect a quantitatively less severe ectopic activation of ARFs. This would suggest that an elevation, as well as a lack, of auxin signaling can result in apical patterning defects. This idea is supported by analyses that showed correlation of fused cotyledon frequency with increased auxin signaling 22. Moreover, apical accumulation of auxin also results in cotyledon fusions 2324. An important distinction between the various genetic and physiological conditions that lead to aberrant cotyledon patterns is that in cases of diminished auxin signaling, the apical defects are accompanied by a reduction in vasculature (for example, bdl, mp) 814. By contrast, in cases of elevated auxin signaling, vascularization remains intact or even increases (for example 2223). The tpl mutant falls into the latter class, because it does not display reduced vascular tissue and can even suppress reduced vasculature of bdl and mp.

Previous work has shown that gain-of-function phenotypes triggered by excess post-embryonic MP activity can be neutralized by the dominant bdl allele 20. The recent work on TPL by Szemenyei et al. 3 underscores this inherently quantitative nature of auxin signaling and clearly demonstrates that quantitative shifts in ARF repression yield very disparate developmental readouts. The temperature-sensitive action of the original tpl allele is a marvelous tool to address this topic in more detail. Using a tool-kit of specifically and/or conditionally expressed dominant-negative TPL-family proteins, dominant-negative AUX/IAAs and various ARF mutants and overexpressors, it is now possible to uncover how the auxin pathway diverged quantitatively in specific contexts to give rise to the multitude of morphological and physiological responses controlled by this hormone.