Previous studies in C. elegans suggested that discrete DNA recognition sites for the DCC called recruitment element on the X (rex) are widely distributed along the X chromosome 8. To find the signature of rex sites, McDonel et al. 5 first tested extrachromosomal arrays comprising multiple copies of large DNA fragments sampled from regions known to bind the DCC, followed by deletion mapping of the DNA fragments to refine the identity of candidate binding sites. In this way they identified for the first time four minimal rex sites for binding, whose sizes range from 115 to 411 bp of the C. elegans X chromosome (Figure 1). When present in extrachromosomal arrays in multiple copies these rex sites have full DCC-binding activity.

The new data support the model of C. elegans DCC action in which the condensin-like DCC is first recruited to individual sites and then spreads or diffuses to neighboring regions to repress gene transcription over the entire X chromosome 5. McDonel et al. 5 also define two short degenerate DNA motifs (A and B) that are clustered within the rex sites (Figure 1). They showed by mutation analysis that motif A is critical for DCC binding, whereas motif B is also important, but to a lesser extent. Intriguingly, these motifs are not X-chromosome-specific and are not even enriched on the X chromosome. Their co-occurrence at high density is essential for full DCC recruitment activity in rex arrays (Figure 1), indicating that it is their clustered distribution on the X chromosome that is critical for their function.

Global recognition of X chromosomes may have evolved in a similar way among different species. In the somatic cells of Drosophila, the single male X chromosome is bound and upregulated by a dosage-compensation complex called the male-specific lethal complex (MSL), which includes specific proteins and two noncoding RNAs transcribed from genes on the X chromosome - RNA on the X 1 and 2 (roX1 and roX2) 1. A number of X-linked sites have affinity for MSL, but to a variable extent 910. It has been proposed that densely clustered sites with a range of affinities for MSL work cooperatively at short and medium distances to recruit the complex to the whole X chromosome 1112.

Two recent analyses, based in one case on the sequence of high-affinity binding sites for MSL and in the other on a compilation of MSL-binding sequences found by whole-genome chromatin immunoprecipitation analyzed by high-resolution genome tiling arrays (ChIP-chip), have identified several recurrent motifs made up of various combinations of degenerate short DNA sequences 1013. Notably, most of these short elements contain GA or CA dinucleotides, and mutation of GAGA repeats in the weakly conserved sequence of around 200 bp in roX1 and roX2 genes, which encode the RNA components of the DCC, disrupts MSL binding to the roX genes 14. Although the presence of these short DNA sequences partially explains MSL binding on the X chromosome, not all binding sites can be predicted. Interestingly, in Drosophila there is no overall enrichment on the X chromosome of the degenerate short DNA sequences that recruit the MSL complex 1013; it is the combination of these sequences that seems to be important - a situation similar to that reported for C. elegans by McDonel et al. 5.

In mammals, the mechanism of X upregulation is not known 15, whereas silencing of one X chromosome in females by X inactivation is well characterized. X inactivation is initiated at the XIST locus located within the X-inactivation center, from which silencing spreads in cis along the chromosome 2. Repeats of the LINE-1 element are enriched on the X chromosome and might act as 'relay elements' to facilitate the spreading of gene silencing 16. Enrichment in specific dinucleotides has also been reported for the mammalian X chromosome 17, but it remains to be determined whether these play any role in silencing, or possibly in upregulation. Segregation of the inactive X chromosome as a body of condensed chromatin (the Barr body) could also facilitate the spreading of silencing, which may be initiated by nucleation of heterochromatin at the center of the Barr body 1819.

The model of McDonel et al. 5 predicts that a cosmid with at least one motif A and one motif B clustered within a window of 600 bp should have DCC-binding activity. Using this simple model, the authors showed that the distribution of predicted 30 kb segments of this type corresponded well to the DCC-recruiting activity of large regions of the X chromosome mapped in previous work 8. Although this model predicted DCC-binding cosmids with 44% accuracy, it is notable that a proportion of the predictions were incorrect, suggesting that other DNA motifs might be involved in DCC binding. Because of the limitation of extrachromosomal array analysis, it will be difficult and time-consuming to search for other rex sites and DNA motifs using this approach. As mentioned above, whole-genome ChIP-chip in Drosophila has revealed the complete DCC-binding pattern along the X chromosome 1320. Such an analysis in C. elegans would generate global DCC-binding profiles and provide clues for the identification of additional rex sites with high binding activity. Additional factors might play a role in DCC recruiting: for example, active transcription and nuclear pore proteins have been shown to affect DCC targeting to the Drosophila X chromosome 2122. Highly expressed X-linked genes are frequent DCC targets, implying either the need for dosage compensation for these active X-linked genes or facilitation of DCC binding due to the open chromatin structure 1320.

Another intriguing finding by McDonel et al. 5 is that motifs A and B are partially functionally redundant despite their distinct DNA sequences. Furthermore, the binding activity of a single type of rex site in an extrachromosomal array is closely correlated with the number of motifs, suggesting that these motifs might work cooperatively to recruit the DCC. How are these clustered short motifs recognized? Do they interact with multiple domains of one protein or with different proteins of the C. elegans DCC simultaneously? It will be important to identify these proteins, or possibly novel proteins, as well as their order of recruitment. Other potential candidates that may recognize the cis motifs are noncoding RNAs. Large noncoding RNAs, such as XIST in mammals and roX in flies, play an important role in directing the DCC to the X chromosome. Interestingly, both XIST and roX RNAs contain functionally redundant elements, which might interact collaboratively with degenerate DNA elements via imprecise base pairing 21223. It will be of great interest to search for noncoding transcripts from the C. elegans chromosomal regions containing rex sites.

Finally, it should be noted that the dissection of C. elegans rex sites by McDonel et al. 5 was carried out mostly using extrachromosomal arrays, which might behave differently from the native rex sites. For example, in mutants of SDC-3, a core protein component of the DCC, another core protein component, SDC-2, still binds to the rex-1 arrays but not to the X chromosome containing native rex sites 5. The nature of these cis-acting DNA elements will need to be ultimately addressed by mutation analysis of endogenous rex sites. It would be interesting to test whether deletion of any rex site is lethal to the animal, affects spreading or recognition of DCC to adjacent regions, or derepresses neighboring X-linked genes. Such studies will greatly expand our understanding of the basis of chromosome-wide recognition of the X chromosome in dosage compensation.