The Navarre Pyrenees area, approximately 2200 km², is situated in the north-east of Navarra and in the south-western part of the Pyrenees (Figure 1). It is a mountainous region with acute unevenness of terrain (from 245 metres over sea-level to 2428 metres at the summit of its highest mountain). The area has a mild oceanic climate in its northern part which shifts to a submediterranean climate type as one moves to the southern areas of Navarre 56; this fact makes it different from other Pyrenean regions (Central and Eastern Pyrenees), which exhibit a basically Mediterranean climate 57. Lithology is varied: limestone mountains dating from the Upper Cretaceous Era rise out of valleys which are composed mainly of Palaeozoic and Eocene flysch (Tertiary Era), with marls and limestone marls from the Mid and Upper Eocene Era towards the western fringe 58.

All this produces a distinctive flora consisting of about 1822 taxa 7, including the species of the adjacent Pyrenean regions. Forests such as those of Fagus sylvatica L. and of Quercus humilis L. are plentiful, as well as of Pinus sylvestris L., Acer opalus Miller, Buxus sempervirens L., Lonicera etrusca G. Santi, Viburnum lantana L., of various species of the Rosa genus 59, and of endemic species such as Campanula ficarioides Timb.-Lagr. ssp. orhyi, Alchemilla iratiana S.E Fröhner or Euphorbia pyrenaica Jord, among others. 7.

Given its biological and cultural richness, the region currently enjoys a good variety of protected areas: 3 integral reservation zones, 8 natural reservation zones, 14 locations proposed as Sites of Community Interest (SCIs) within the Natura network, and 11 flower areas introduced by Blanco 60 in his areas of botanical interest (such as the Irati forest, one of the most important beech woods in Europe), along with a further 31 zones under diverse forms of biological protection.

In the seven valleys of the Navarre Pyrenees human settlement dates back 7,000 years, as has been proven by archaeological findings in the Aizpea Cave 61. The first inhabitants in the region, the Vascones, were influenced by several cultures that sought to settle in this territory as well, such as the Romans and the Visigoths. Nowadays, the population in this area lives in small villages and houses scattered across the hills and valleys. They earn a living through cattle raising, hand-made exploitation of forests and the cultivation of a variety of vegetables for consumption. Yet, these activities have been reduced in the shift towards computer and service industries and because of emigration to large centres of population. Despite the fact that cultural traditions have remained well-rooted in the region, only 10% of the population speaks 'Euskara', the language of the Basque ethnic group; the percentage of Euskara speakers was higher at other times in recent history (18^th and 19^th centuries) 50, a fact which influences different aspects of the present culture, including ethnobotany.

All these biotic and cultural factors, and the need for a scientific review of the pharmacology of the Navarre Pyrenees, constitute the primary motive of this study, whose objectives are:

-to compile an ethnobotanical and medical catalogue of the Navarre Pyrenean Region;

-to perform a quantitative analysis of the results and to compare it with other ethnobotanical studies of the Pyrenees region;

-to carry out a review of the drugs used within the community through study of the monographs published by WHO, ESCOP and the E Commission of the German Department of Health, institutions responsible for the safety and efficacy of medicinal plants, in order to assess the official validity of the pharmacology of the Navarre Pyrenees.

Botanical remedies of the folk pharmacopoeia in the Pyrenean region of Navarra, comprise 92 species (see additional file 1). Of these species, 39 species were cited by three or more informants. This does not mean that a cure or species mentioned by only one or two informants is without value, but may simply reflect the disappearance of particular knowledge; the fact of being reported on a relatively low scale may also mean that these plants are worth of further study.

The predominating botanical families are Asteraceae (15 species), Lamiaceae (13 species) and Rosaceae (8 species). Only a few botanical families mentioned by the informants comprise between one-third and one-half of the total number of plants cited. This corresponds to other ethnobotanical studies carried out using the same method in the Pyrenees 44. It is not surprising, because these families are well represented in the Pyrenees flora and contain some very common plants (the Asteraceae family is the most important of them, and Lamiaceae and Rosaceae are among the top seven) 7. As confirmed by Johns et al. 72 and other authors 184144, the more common a plant is in an area, the greater the probability of its popular use.

Sambucus nigra is the most reported species and almost all its organs are used and many of their applications are also mentioned in Bonet et al. 44, Agelet and Vallès 42 and Vallès et al. 15. Apart from the fact that different parts of the plant are used, harvesting of the plant, practically all year round, has been reported, which encourages its continued use generation after generation. Other plants in significant use, as recorded in other studies about the Pyrenees, are: Santolina chamaecyparissus ssp. squarrosa and Urtica dioica (stinging nettle).

The parts of the plants most used for medicinal purposes are, in descending order: leaves, flowers (including floral summits and flowering heads), aerial parts and fruits, results similar to those observed by Bonet et al. 44 and very similar to those obtained by Cerdanya 4546. According to Bonet et al. 44, the reason why leaves are used most is because they are easily accessible. Besides, recalling the line of reasoning proposed by Johns et al. did is remember ('the more common a plant in the area, the higher its probability of use'), if the leaf is the most accessible or common part in the flora of the area, and the most abundant as well, it is more likely to be used. It was also observed that the interviewees showed a marked inclination to using leaves in the process of identifying and distinguishing medicinal plants. Thus, if the leaf is a key factor in the identification of plants, and of frequent and easy access, it follows that it would be more used than other plant organs.

The percentage of external uses (54.4%) is lightly higher than internal uses (45.6%) and infusion is the main method of preparation for oral administration, as reported in the majority of the Pyrenean studies 4144. It is also important to point out that it is not always easy to differentiate this procedure from decoction on the basis of information given in interviews, a fact confirmed by several authors 94573.

Regarding external uses, the most common is the poultice, according to the data collected in this study and in others on the central and eastern Pyrenees. 414448. In the Navarre Pyrenees, there were 20 references mentioned maceration as a method of preparation. The most usual is maceration with anisette (with a use entirely internal and digestive), as in the case of the preparate called 'patxaran', the most famous and characteristic liquor not only of the Pyrenean region but also of Navarra as a whole. Its production and consumption has also been reported in certain neighbouring villages and towns in the central Pyrenees 48. Other liquors with digestive uses are produced; for example "patxaka" are produced, which is prepared from anisette and rosehips from several species of the genus Rosa. Maceration in alcohol (always used externally), oil or anisette and its traditional use have been preserved due to the easy process of preparation and the long durability of the products.

Only a few medicinal species are used as edible plants. This fact is widely different to the rest of Pyrenees regions. The explanation is that the number of medicinal food plants from the rest of the Pyrenees is similar to other European Mediterranean areas, whereas our data alike other Temperate areas from northern Iberian Peninsula 1620. In addition the informants clearly distinguish between culinary and medicinal uses alike other Temperate areas, although these use may sometimes overlap 2021.

Another important issue to be pointed out is the fact that whereas in other studies about the Pyrenees, the use of liquors or teas has been regarded as falling between medicinal and edible uses because of its social character 44, this is not the case in this study because, although the use of some liquors has been socialized, these liquors continue to be consumed for medicinal purposes.

Almost all of the species are used alone, very few mixtures have been identified, such as the use of garlic and verbena in the production of poultices, or the use of garlic and elder in the preparation of ointments. The widespread use of garlic, mainly in dermatological preparations, shows the popular valued attributed to this species, although interviewees pointed out that the plants accompanying the garlic usually perform the most significant role.

In Navarre Pyrenees the number nine is repeated in methods concerning the popular administration of plants, which are always given for nine consecutive days (known as a "novena") during the seasons of autumn and spring, as also happens in other cultures in the Pyrenees 414244. From the ethnographic point of view this phenomenon reflects the idea that the number nine represents a magical number in traditional medicine 7778 or, as Bonet et al. 44 indicate, the idea that the use of the plant over more days could have harmful effects for the organism.

The use of medicinal plants in certain symbolic rites still occurs in the Navarre Pyrenees. The informants do not positively state whether the healing principle is the rite or the plant. Nevertheless, as happens in other places in the Pyrenees, people still place certain previously blessed species 48 on the doors of the houses, to protect them from illness. This has no scientific basis nowadays, but folklore continues to give equal importance to both conceptions of illness and remedy.

Informants used 112 local names to refer to the 92 medicinal species catalogued, 14 of them in Basque (12,5%), a slightly higher percentage than that of Basque-speakers (10%). Among the Spanish names 10 refer to the place where the plants are recollected ("manzanilla de monte", mountain camomile, "té de roca", rock tea); others refer to their attributed use ("hierba para las almorranas", herb for piles, because of its use on haemorrhoids, "hierba para las piedras del riñon" herb for kidney stones); or its resemblance to animals ("patamula", mule's leg; reported as well in the neighbouring Pyrenean region 48, "pedo de lobo" wolf's fart or "cola de caballo" horse's tail). Special mention should be made of "cabardera" or "cabarda" to name bushes from the genus Rosa, most likely with the same phytonomous origin as that of the "gabarderas" reported in the ethnobotanical study of the Central Pyrenees 48. These references have also been found among the Basque names recorded, such as plant names based on their use "pasmobelarra", amazement herb, or "iodobelarra", iodine herb, because of its resemblance to this chemical product. Mistletoe is commonly known in the area as "bizko", "migula", "mihura" (from Basque "mi", a corruption of "mamia", flesh or pulp, and "ura", water, which refers to the fruit 79, or the name "patxaran", which comes from the word "basarana" "basoa", forest, and "arana" plum: forest plum. A name related to the sun, "eguzkilore", or sun thistle 80, has been reported as well, which reflects the close relationship that ancient dwellers had with their environment and is used to protect against "evil spirits" and illnesses at night.

A total of 200 popular uses have been compiled, in which dermatology is the most frequently cited category, followed by those categories related to gastrointestinal problems and the respiratory tract. In other regions of the Pyrenees more or less the same uses have been recorded, digestive and dermatological categories being likewise the most important ones 41444648.

This is not surprising, given the fact that, as Bonet and Muntané mention 4445, the way work and life is led in rural areas and the lack of health and hygienic conditions, encouraged the search for natural remedies to cure infected wounds caused in daily life, or tisanes that helped the digestion of high calorie meals eaten to withstand cold temperatures. It should be borne in mind that most of the informants lived an exclusively rural way of life until approximately two decades ago.

Another point of interest is that traditional cures are usually limited to the treatment of mild and chronic diseases, such as Reuter and Bonet studied in other regions [mentioned in 44]. However, it is known that quacks from the Navarre Pyrenees usually treated serious diseases with medicinal plants when people were not able to treat them with the remedies that are presented above. Although those quacks have been reported in many European ethnobotanical research studies 8182, it is not possible to find them on Navarre Pyrenees as they died.

Table 2 shows the results of some quantitative data from the Navarra Pyrenees, as well as the results of other studies performed in the Pyrenees. The radius MP/Km² is slightly higher than that of the neighbouring Pyrenean region [mentioned in 48], but clearly lower than some results from the eastern Pyrenees, with a more Mediterranean character, which may have a strong influence on the presence and use of more species in a region milder than the Navarre Pyrenees.

The pharmacological ethnobotany index (EI) is clearly lower (5,05%) than the other with which this one has been compared, which suggests:

a) from the floral data of an area larger (the Pyrenees and the Prepyrenees) than that of this study (only the Pyrenees), the resulting EI is affected and shows a lower value than the actual one (EI = 5,05);

b) it is possible that the data reflects a cultural loss in the ethnobotanical and medical knowledge in the area, as suggested by the relatively low number of species (39) reported by at least three of the informants;

c) according to Mesa-Jiménez 83, a smaller number of medicinal plants used by a community means a higher rate of validation of those plants, because their efficacy has meant that other remedies have not been sought among other species, and therefore shows a greater level of adaptation of the inhabitants to their environment. In order to check if this study reflects this theory, the Shannon-Wiener index and the Equitability Index have been calculated. H' = 3,855, which means high diversity because the maximum value (H_max) is 4,521; and E = 0,85 (value close to the maximum, which is 1). Therefore, these indexes show that the level of adaptation to the environment is low, according to Mesa-Jimenez's argument.

Besides, the FIC value is 0,65. The value of this index (ranging from 0 to 1) for the area studied, despite being high, is considerably lower than values calculated in several areas of the Iberian Peninsula: 0,85 and 0,91 for a Portuguese and a Catalan region respectively 3614.

For each therapeutic category established, Table 3 shows the comparison between the popular use of drugs and their evaluation by the WHO, ESCOP and the E Commission, as well as the references to each plant in relation to each therapeutic application. In this table, taxa are ordered alphabetically and there are missing species that is, species with no monograph history.

Of the 92 species in the ethnobotanical catalogue, 39 do not appear in the published monographs, which indicates that their safety and efficacy are not officially recognized because of a lack of scientific study, despite the fact that several bioactive compounds and active ingredients of some plants have been recognized by a number of authors, as outlined below. A third of all plants belongs to the category related to dermatological disorders, the most frequently cited category in the Navarre Pyrenees. The extent to which the Navarre Pyrenees ethnopharmacology has been officially reviewed is 57,6% of the total, which suggests a large scope of research still to be done.

The traditional ointment of the second bark of Sambucus nigra is barely mentioned in the scientific bibliography, could be a possible route for further pharmacological study because preliminary studies on the elder bark 84 have confirmed the presence of non-toxic ribosome-inactivating protein (RIP), leading to the inhibition of protein synthesis. According to Uncini-Manganelli et al. 85 and Girbes et al. 84 conjugation of RIP to monoclonal antibodies is a promising tool for cancer therapy. So a far-reaching study of this plant to test these biological properties may be of significant interest.

On the other hand, attention should be focused on the use of plants known popularly as "manzanilla" (chamomile), which correspond to the following species: Chamaemelum nobile, Santolina chamaecyparissus ssp. squarrosa and Tanacetum parthenium, generally used for gastrointestinal disorders. The difference which was observed between these three taxa is that from valley to valley the taxon known as "manzanilla" varies. Thus Chamaemelum nobile is used in the most western part of the region (which has the most humid climate), Santolina chamaecyparissus in the most Eastern parts (bordering the Central Pyrenees Region, a more Mediterranean climate), and Tanacetum parthenium only cultivated in kitchen gardens in the several villages of the most eastern valleys. This factor can be explained by the influence of the climate on the selection of plants named and used as chamomiles. These similar species, and with the same use, may be used interchangeably, depending on their accessibility and availability, among other reasons 86.

Hypericum perforatum is cited as an excellent plant for the treatment of diarrhoea. It is more commonly used for this gastrointestinal problem than for other purposes such as, for example, as a tranquillizer, as happens frequently in other cultures of the world. This plant was cited along with Verbena officinalis as a tranquillizer; taken during autumn and winter to raise the spirits because of the depression or physical decline which some inhabitants of this region suffered during these period of the year. Moreover, this plant is usually harvested on St. John's Eve along with Sambucus nigra, Verbena officinalis and Rosa canina, among others; this harvest is regarded as an ancestral custom, and the magical or medicinal properties of these plants are believed to increase that night.

Verbena officinalis is also used in folk medicine as an expectorant and anti-rheumatic and anti-inflammatory substance. Although some of these uses have been proven scientifically 87, WHO, ESCOP and the E Commission have not published any monographs that ensure their safe and effective application.

Regarding the mistletoe (Viscum album), all the informants agreed that the only plant which "worked" was the one which appears on species such as blackthorn (Prunus spinosa), apple (Malus sylvestris) or other species in the rose family. Viscum album, is characterized by its action on the cardiovascular system. Nevertheless, this is the sub-species album 65, which is a parasite on plants of the rose family, and so it would seem that the active compounds could vary according to the particular sub-species of Viscum album in question.

Scrophularia auriculata is a species barely mentioned in terms of traditional medicine in scientific bibliography. However, studies revealing glycoterpenoids in its chemical composition have been carried out 88. These compounds reduce inflammatory injury and suppress cellular infiltration, which would corroborate the traditional application of this plant, although it has not yet been officially approved, as is the case also with Dorycnium pentaphyllum and Saxifraga longifolia, which have not been the object of no critical evaluation of their scientific properties; and therefore, no monographs have been published and this study marks the first time they have been recorded in the context of medicinal purposes.

Rosa sp., Viola sp., Ocimum basilicum, Origanum vulgare and Nerium oleander are the only species about which negative monograph have been published (71), which advise against prescribing the fruit on the grounds that its therapeutic effects have not been proven sufficiently. Another plant about which the E Commission has reported negatively is Fragaria vesca which, although scarce in the zone, it is used to relieve 'prostate discomfort'. Its popular use as a diuretic and antigout treatment has been recorded in other areas, using leaves and fruits respectively 8990, and the beneficial effect of this species (among others) has been scientifically proven for the post-operatory period in prostatic adenomectomy 91.

In this context, therefore, more detailed, pharmacological study of these and other interesting species may be of considerable value, not only to validate their use and the future validation of traditional medicine in rural areas, but also, as recommended by WHO 92, because they may provide support for sustainable development projects in these areas, as mentioned by Pieroni et al. 8193.