BALB/c severe-combined immunodeficient (scid) mice were inoculated with 2 × 10⁵ pfu per eye of an HSV-1 ICP0^- virus, n212 (described in Table 1). At 2 and 12 hours post inoculation (p.i.), infectious virus was not detectable in the ocular tear film of mice. At 24 hours p.i., an average of 3000 pfu of n212 was recovered from the eyes of scid mice (black circles in Fig. 2A). Replication of the ICP0^- virus remained low to undetectable between days 3 and 70 p.i., and the n212 infection produced no disease. Thus, 100% of n212-infected scid mice remained healthy and survived for 70 days p.i. (red line in Fig. 2A). Secondary challenge with wild-type HSV-1 strain KOS on day 70 p.i. verified that n212-infected scid mice had not mounted an adaptive immune response to HSV-1. KOS sustained high levels of replication in the eyes of scid mice, and produced a uniformly lethal infection (Fig. 2A).

Multiple experiments confirmed that the ICP0^- virus n212 was avirulent in scid mice, whereas the wild-type KOS strain produced uniformly lethal infections in scid mice (Fig. 2B). Moreover, n212 appeared to rapidly exit the productive cycle of viral replication in scid mice based on i. low to undetectable levels of infectious virus in the tear film of scid mice between 3 and 70 days p.i., ii. undetectable levels of infectious virus in homogenates of eyes or trigeminal ganglia (TG) at 35 or 70 days p.i. (n = 10 tissues per time point), and iii. the fact that n212-infected scid mice remained indistinguishable from uninfected mice for more than 2 months p.i.

To determine if the IFN-induced antiviral state 1920 or the IFN-induced pro-myelocytic leukemia (PML)-associated protein 21 is relevant to the innate mechanisms by which mice rapidly repress HSV-1 ICP0^- null mutants in vivo, the acute ocular replication of an ICP0^- virus was compared in wild-type strain 129 mice, recombination-activated gene 2^-/- (rag2^-/-) mice, PML^-/- mice, or stat1^-/- mice (described in Table 1). Following inoculation with 2 × 10⁵ pfu per eye of the HSV-1 ICP0^- virus n212, ~1000 pfu per eye of virus was recovered from all strains of mice at 24 hours p.i. (Fig. 3A). On day 3 p.i., titers of infectious n212 were ~1000-fold higher in the eyes of stat1^-/- mice relative to the other strains of mice (Fig. 3A). While rag2^-/- mice survived n212 infection for >60 days, n212 infection was lethal in 3 of 4 stat1^-/- mice by day 12 p.i. Loss of Stat 1 alleviated host repression of n212, but loss of PML did not produce a comparable effect. PML^-/- mice repressed n212 replication at the site of ocular inoculation with the same kinetics as wild-type mice and rag2^-/- mice (Fig. 3A).

The n212 virus bears a small 14 bp linker insertion in the ICP0 gene (Fig. 4), which can revert to a wild-type ICP0 gene by excision of the linker sequence in vivo (unpublished observation). To verify that an ICP0^- virus itself, as opposed to a wild-type revertant, was capable of producing disease in stat1^-/- mice, a second experiment was performed with the ICP0^- virus, 0^--GFP (Fig. 4; described in Table 1). At 24 hours p.i., ~1000 pfu per eye of 0^--GFP was recovered from the eyes of wild-type mice, rag2^-/- mice, stat1^-/- mice, or rag2^-/- stat1^-/- mice (Fig. 3B). On day 3 p.i., titers of infectious 0^--GFP were ~1000-fold higher in the eyes of stat1^-/- mice and rag2^-/- stat1^-/- mice relative to wild-type mice or rag2^-/- mice (Fig. 3B). At all times p.i., the virus recovered from stat1^-/- mice and rag2^-/- stat1^-/- mice retained the GFP insertion in the ICP0 gene (Fig. 4) based on the GFP⁺ phenotype of plaques that formed in plaque assays. In this experiment, 0^--GFP infection was lethal in 100% of stat1^-/- mice and rag2^-/- stat1^-/- mice by day 12 p.i.

In multiple experiments, the ICP0^- viruses n212 and 0^--GFP did not produce disease in strain 129 mice, rag2^-/- mice, and PML^-/- mice, and 100% of the mice survived for 60 days p.i. (Fig. 3C). In contrast, n212 and 0^--GFP produced lethal infections in 50 to 100% of stat1^-/- mice and in 100% of rag2^-/- stat1^-/- mice (Fig. 3C). Thus, the IFN-activated Stat 1 transcription factor was required for rag2^-/- mice to rapidly repress the replication of ICP0^- viruses in vivo.

Given the severity of the immunodeficiency of rag2^-/- stat1^-/- mice, mortality in this strain may have been due to secondary infections introduced by corneal scarification and/or human handling. To address this possibility, a series of in vitro and in vivo experiments were performed comparing the ICP0^- virus, 0^--GFP, to the replication-defective HSV-1 ICP4^- virus, n12 (described in Table 1). In vitro, an inoculum of 2.5 pfu per cell of 0^--GFP replicated relatively efficiently in Vero cells, whereas the ICP4^- virus produced no viral progeny (Fig. 5A). When Vero cells were treated with the IFN-α/β receptor agonist, IFN-β, both 0^--GFP and the ICP4^- virus failed to produce viral progeny (Fig. 5B). In contrast, wild-type HSV-1 resisted repression by IFN-β and was only transiently delayed in its replication relative to untreated cells (Fig. 5B). Thus, ICP0 was required for HSV-1 replication when cultured cells were exposed to the Stat 1 activator, IFN-β.

In vivo, 0^--GFP replicated to high titers in the eyes of rag2^-/- stat1^-/- mice, acute swelling of periocular tissue occurred, and none of the mice survived beyond day 11 p.i. (Fig. 5C). In contrast, the ICP4^- virus failed to replicate in rag2^-/- stat1^-/- mice or rag2^-/- mice, and all of the ICP4^- virus-infected mice remained healthy for the 30-day test period (Fig. 5C and 5D). In rag2^-/- mice, which retained a functional Stat 1 pathway, the ocular replication of 0^--GFP was rapidly repressed and 100% of 0^--GFP-infected rag2^-/- mice remained healthy for the 30-day observation period (Fig. 5D). Thus, the pathogenesis of 0^--GFP infection observed in rag2^-/- stat1^-/- mice appeared to be the result of unchecked viral replication, and was not the result of an unanticipated infection with the flora of the mice or their human handlers.

To determine if the Stat 1-dependent host response was relevant to wild-type HSV-1 infection, strain 129 mice, rag2^-/- mice, stat1^-/- mice, or rag2^-/- stat1^-/- mice were inoculated with 2 × 10⁵ pfu per eye of KOS-GFP, a GFP-expressing recombinant of strain KOS (described in Table 1). On day 1 p.i., titers of KOS-GFP were equivalent in the eyes of all groups of mice (Fig. 6A). On day 3 p.i., KOS-GFP titers were ~100-fold greater in the eyes of stat1^-/- and rag2^-/- stat1^-/- mice relative to wild-type and rag2^-/- mice (Fig. 6A). Likewise, GFP fluorescence was nearly undetectable in the eyes of wild-type and rag2^-/- mice on day 3 p.i., but persisted in the eyes of stat1^-/- mice and rag2^-/- stat1^-/- mice (Fig. 6B). Infectious KOS-GFP titers were ~10-fold higher on day 5 p.i. in the TG of stat1^-/- and rag2^-/- stat1^-/- mice relative to wild-type and rag2^-/- mice (Fig. 6A). Likewise, GFP fluorescence emanated from large tracts of cells in the TG of stat1^-/- and rag2^-/- stat1^-/- mice on day 5 p.i., whereas the TG of wild-type and rag2^-/- mice possessed discrete foci of GFP fluorescence (Fig. 6B). In wild-type and rag2^-/- mice, only limited spread of KOS-GFP to the hindbrain of mice was observed on days 5 and 7 p.i. (Fig. 6A, 6B). In contrast, GFP expression was evident in the hindbrain of 17 of 20 stat1^-/- and rag2^-/- stat1^-/- mice on days 5 and 7 p.i. (Fig. 6B). Thus, an innate Stat 1-dependent host response is necessary to prevent extensive spread of KOS-GFP infection from the corneal epithelium to the central nervous system of mice.

To determine if host IFNs are the principal activators of Stat 1-dependent repression of HSV-1, the progression of KOS-GFP (ICP0⁺) or 0^--GFP (ICP0^-) infection was compared in mice of the following genotypes: 1. wild-type, 2. rag2^-/-, 3. ifngr^-/- (IFN-γ receptor-null), 4. ifnar^-/- (IFN-α/β receptor-null), 5. ifnar^-/- ifngr^-/-, 6. stat1^-/-, 7. rag2^-/- stat1^-/-, or 8. rag2^-/- ifnar^-/- (described in Table 1). Following inoculation with 2 × 10⁵ pfu per eye of KOS-GFP, similar levels of GFP fluorescence were observed in the corneas of mice at 36 hours p.i. (Fig. 7A). By 60 and 84 hours p.i., GFP fluorescence was nearly undetectable in the corneas of wild-type, rag2^-/-, and ifngr^-/- mice (Fig. 7A). All strains of mice with a defect in the ifnar or stat1 genes failed to limit KOS-GFP spread, and thus GFP fluorescence was still evident throughout the cornea at 84 hours p.i. (Fig. 7A). Likewise, KOS-GFP titers were an average 10- to 300-times higher in the tear film of ifnar^-/-, ifnar^-/- ifngr^-/-, stat1^-/-, rag2^-/- stat1^-/-, and rag2^-/- ifnar^-/- mice relative to wild-type mice at 72 hours p.i. (Table 2). Stat1^-/- mice, rag2^-/- stat1^-/- mice, and rag2^-/- ifnar^-/- mice died of a typical viral encephalitis 8 to 9 days p.i., based on the symptoms of hunched posture, ataxia, and hyperexcitability, which preceded death by ~18 hours (Table 2). Ifnar^-/- ifngr^-/- mice succumbed to KOS-GFP infection just 5.2 ± 0.1 days p.i. (Table 2), and presented with acute lethargy ~8 hours prior to death. Consistent with the findings of Luker, et al. 19, dissection at the time of death revealed that the livers of ifnar^-/- ifngr^-/- mice were visibly discolored and the entire liver mass was GFP⁺ (not shown). Thus, fulminant viral infection of the liver, and presumably liver failure, appeared to be the primary cause of death in KOS-GFP-infected ifnar^-/- ifngr^-/- mice.

Following inoculation with 2 × 10⁵ pfu per eye of 0^--GFP, similar levels of GFP reporter gene expression from the ICP0 gene (diagram in Fig. 4) were observed in the corneas of mice at 36 hours p.i. (Fig. 7B). By 60 and 84 hours p.i., GFP fluorescence decreased to nearly undetectable levels in the corneas of wild-type, rag2^-/-, and ifngr^-/- mice (Fig. 7B). All mice with a defect in the ifnar or stat1 genes failed to limit 0^--GFP spread, and GFP fluorescence was still evident throughout the cornea at 84 hours p.i. (Fig. 7B). Likewise, 0^--GFP titers were an average 300 to 1000 times higher in the tear film of ifnar^-/- ifngr^-/-, stat1^-/-, rag2^-/- stat1^-/-, and rag2^-/- ifnar^-/- mice relative to wild-type mice at 72 hours p.i. (Table 2). Most of the mice that shed titers of >1000 pfu per eye of 0^--GFP on day 3 p.i. died of the infection. Rag2^-/- mice survived 0^--GFP-infection for 60 days and exhibited no symptoms of disease. In contrast, rag2^-/- stat1^-/- mice and rag2^-/- ifnar^-/- mice uniformly succumbed to 0^--GFP infection (Table 2). Thus, the IFN-α/β receptor and downstream Stat 1 transcription factor are essential for the innate host response that represses 0^--GFP replication in rag2^-/- mice.

A subset of neurons synthesize LAT RNAs as soon as HSV-1 infection spreads to the TG 22. The relative frequency of LAT⁺ neurons and viral antigen (Ag)⁺ neurons was compared in the TG of wild-type mice, rag2^-/- mice, stat1^-/- mice, or rag2^-/- stat1^-/- mice inoculated with HSV-1 strain KOS (2 × 10⁵ pfu per eye). On days 3 and 5.5 p.i., the frequency of LAT⁺ neurons was equivalent in all strains of mice, and approximately 1 to 3 LAT⁺ neurons were observed for every 1000 TG neurons counted (Table 3). Thus, the process by which HSV-1 rapidly establishes latent infections in this subset of neurons is not dependent on lymphocytes (as previously shown; Ref. 22) or the Stat 1 signaling pathway.

During the acute infection, HSV Ag⁺ neurons were >100-fold more abundant in TG than LAT⁺ neurons (Table 3). On day 3 p.i., the frequency of HSV Ag⁺ neurons was equivalent in all groups of TG, and ~100 to 300 HSV Ag⁺ neurons were observed for every 1000 TG neurons analyzed. On day 5.5 p.i., HSV Ag⁺ neurons were twice as abundant in the TG of rag2^-/- mice relative to strain 129 mice (Table 3). On day 5.5 p.i., HSV Ag⁺ neurons were too numerous to count in the TG of stat1^-/- and rag2^-/- stat1^-/- mice and viral CPE severely compromised the integrity of the tissue. Thus, consistent with other results, the Stat 1 signaling pathway was essential to restrict the spread of wild-type HSV-1 from the site of inoculation to the TG.

The relative efficiency with which an ICP0^- virus establishes latent infection in the TG of wild-type mice, ifnar^-/- mice, ifngr^-/- mice, or stat1^-/- mice was compared to wild-type HSV-1 strain KOS. All strain 129 mice inoculated with 2 × 10⁵ pfu per eye of KOS survived the acute infection, as did all strain 129 mice, ifnar^-/- mice, or ifngr^-/- mice inoculated with 2 × 10⁵ pfu per eye of 0^--GFP (Table 4). Despite a ten-fold reduction in viral inoculum, 100% of rag2^-/- ifnar^-/- mice, 79% of ifnar^-/- ifngr^-/- mice, and 50% of stat1^-/- mice succumbed to acute infection following inoculation with 2 × 10⁴ pfu per eye of 0^--GFP (Table 4).

HSV-1 genome loads per TG were analyzed by competitive PCR amplification of a virion protein 16 (VP16) gene sequence. VP16 PCR products were not amplified from uninfected TG DNA, but were consistently amplified from HSV-1 infected TG DNA samples (Fig. 8A). VP16 PCR products amplified from a VP16 plasmid DNA dilution series defined the relationship between PCR product yield and viral genome copy number per PCR (Fig. 8A and 8B). Viral genome load per TG was evaluated in 0^--GFP infected mice that developed encephalitis at day 9 p.i. (Fig. 8A). VP16 PCR product amplification was well outside the quantitative range of the PCR assay, but demonstrated that the TG of encephalitic rag2^-/- ifnar^-/- mice, ifnar^-/- ifngr^-/- mice, or stat1^-/- mice all possessed in excess of 10⁷ HSV-1 genomes per TG on day 9 p.i. (Fig. 8A).

HSV-1 latently infected mice were sacrificed at day 40 p.i. to measure viral genome load per TG. KOS-latently infected strain 129 mice contained an average of 3.0 × 10⁵ viral genomes per TG (Fig. 8C; Table 4). In strain 129 mice and ifngr^-/- mice, the number of latent 0^--GFP genomes per TG was ~40% of the wild-type level achieved by KOS. In ifnar^-/- mice, the number of latent 0^--GFP genomes per TG was ~115% of the wild-type level. In stat1^-/- mice (inoculated with a ten-fold lower dose of 0^--GFP), the number of latent 0^--GFP genomes per TG was ~60% of the wild-type level (Fig. 8C; Table 4). If one considers the primary data, 0^--GFP established latent infections in 50% of ifnar^-/- mice and stat1^-/- mice that met or exceeded the latent viral genome load per TG achieved by KOS in strain 129 mice (Fig. 8A).

The efficiency with which latent KOS (ICP0⁺) and 0^--GFP (ICP0^-) reactivated from TG explants was compared. TG were harvested from mice on days 38 and 39 p.i., heat stressed at 43°C for 3 hours, co-cultured with L7 cell monolayers for 14 days, and were homogenized on day 14 to test for the presence of infectious virus within each TG. Of the KOS-latently infected TG harvested from strain 129 mice, 11 of 16 TG explants produced infectious virus that spread to co-cultured L7 cells (Table 4), and 14 of 16 TG homogenates contained infectious KOS on day 14 post explant (Table 4). Of the 0^--GFP-latently infected TG harvested from strain 129 mice, ifngr^-/- mice, ifnar^-/- mice, ifnar^-/- ifngr^-/- mice, or stat1^-/- mice, 0 of 98 TG explants produced infectious virus that spread to co-cultured L7 cells (Table 4). In contrast, infectious 0^--GFP was detected in 2 of 16 ifnar^-/- TG homogenates and 2 of 22 stat1^-/- TG homogenates on day 14 post explant (Table 4). Reactivation of 0^--GFP was detected in none of the other TG homogenates on day 14 post explant (Table 4). Thus, consistent with previous results, even when wild-type HSV-1 and ICP0^- viruses establish equivalent latent infections (e.g., ifnar^-/- mice; Table 4), ICP0^- viruses are severely impaired in their capacity to reactivate from latently infected TG explants 2324.

Interferon-sensitive ICP0^- viruses have not been considered for their potential to vaccinate against herpetic disease. The efficacy with which an ICP0^- virus or ICP4^- virus induced protective immunity against lethal HSV-1 challenge was compared. Strain 129 mice were inoculated with 2 × 10⁵ pfu per eye of 0^--GFP or an ICP4^- virus. The ICP0^- virus replicated transiently, but titers of 0^--GFP in ocular tear film remained low to undetectable between days 3 and 30 p.i. (Fig. 9A), and the ICP4^- virus failed to replicate (Fig. 9B). As anticipated, 100% of 0^--GFP- or ICP4^- virus-infected mice survived and remained healthy for 30 days p.i. (Fig. 9A, 9B). On day 30 p.i., these mice were challenged with 2 × 10⁵ pfu per eye of HSV-1 strain McKrae. In 0^--GFP-infected strain 129 mice, one of six mice shed detectable virus at 24 hours post-challenge (Fig. 9A). All of the 0^--GFP-infected mice remained healthy until the conclusion of the experiment, 30 days after secondary challenge with McKrae (Fig. 9A). In ICP4^- virus-infected mice, McKrae replicated to high titers in the eyes of mice, and progressed to fatal encephalitis within 5.8 ± 0.2 days (Fig. 9B).

Equal numbers of rag2^-/- mice were included in this experiment. Inoculation of rag2^-/- mice with 0^--GFP or the ICP4^- virus produced an equivalent pattern of viral shedding as was observed in strain 129 mice between days 1 and 30 p.i. (Fig. 9C and 9D). On day 30 p.i., rag2^-/- mice were secondarily challenged with McKrae. McKrae replicated to high titers in rag2^-/- mice that were first inoculated with 0^--GFP or the ICP4^- virus (Fig. 9C and 9D). In ICP4^- virus-infected rag2^-/- mice, McKrae infection progressed to fatal encephalitis by 5.7 ± 0.1 days post challenge (Fig. 9D). In 0^--GFP-infected rag2^-/- mice, McKrae infection required 9.0 ± 0.5 days to progress to fatal disease (Fig. 9C), suggesting that the ongoing innate response to 0^--GFP infection delayed the progression of McKrae infection to a fatal encephalitis. Therefore, while 0^--GFP infection is avirulent in rag2^-/- mice, 0^--GFP cannot induce protective immunity against HSV-1 in animals that lack a lymphocyte-driven adaptive immune response.

Loss of IFN-α/β receptors in ifnar^-/- mice compromised innate resistance to HSV-1, but the defect was not as profound as observed in stat1^-/- mice. Thus, on day 3 p.i., ifnar^-/- mice shed 30- to 100-times less infectious virus per eye than stat1^-/- mice (Table 2). Likewise, ifnar^-/- mice uniformly survived ICP0^- viral infections with little disease (Table 2), whereas 50% of stat1^-/- mice died of 0^--GFP infection and all of the survivors were frankly diseased. The capacity of rag2^-/- mice to repress 0^--GFP replication was clearly dependent on the IFN-α/β receptor and Stat 1, because 0^--GFP produced uniformly fatal infections in rag2^-/- ifnar^-/- mice and rag2^-/- stat1^-/- mice. Mice that lacked IFN-α/β receptors and IFN-γ receptors failed to suppress the replication of wild-type or ICP0^- viruses at the site of inoculation. Because the extent of the defect was similar to that observed in stat1^-/- mice, the combined activities of IFN-α/β and IFN-γ appear to activate the Stat 1-dependent host response that limits HSV-1 spread in vivo.

The innate resistance of host structural cells to HSV-1 appears to be compromised in the absence of Stat 1 or IFN-α/β receptors, presumably because these cells fail to mount an antiviral state (Fig. 7). However, this simple explanation does not account for all of the observations presented herein. For example, HSV-1 produced a rapid and fatal disease in ifnar^-/- ifngr^-/- mice, which as described by Luker, et al. 19, appeared to be a viral hepatitis. HSV-1 infection rarely causes hepatitis in humans 25, and liver infection is not a prominent feature in animal models. Moreover, stat1^-/- mice infected with wild-type HSV-1 died of encephalitis two to three days later. This divergence in the outcomes of infection in ifnar^-/- ifngr^-/- mice and stat1^-/- mice draws attention to an important caveat of studies in knockout mice: despite the targeted nature of the genetic mutations, loss of a single protein may have pleiotropic effects. For example, basic immune functions such as hematopoiesis or leukocyte activation may be altered by combinations of these mutations. Alternatively, the non-permissiveness of leukocytes for HSV-1 replication may be dependent on the combined activities of IFN-α/β receptors and IFN-γ receptors 2627. Thus, the fulminant liver infection that develops in HSV-1-infected ifnar^-/- ifngr^-/- mice may be due to uncontrolled HSV-1 replication in at least one leukocyte subset, which would confer upon HSV-1 the unusual property of blood-borne spread to the liver. Given such possibilities, the results of the current study must be interpreted with a certain degree of caution.

A subset of neurons express LAT RNAs, but not viral proteins, when HSV-1 first enters the TG on day 3 after ocular inoculation 22. Comparison of the numbers of LAT⁺ neurons in stat1^+/+ versus stat1^-/- mice indicated that this phenomenon is not Stat 1-dependent (Table 3). Thus, the Checkpoint Model fails to explain how HSV-1 establishes latent infections in the subpopulation of stat1^-/- neurons that are LAT⁺ on days 3 and 5.5 p.i. (Table 3). Although LAT⁺ neurons were certainly present in stat1^-/- mice, HSV Ag⁺ neurons were 100- to 500-fold more abundant between days 3 and 5.5 p.i. (Table 3). On day 5.5 p.i., stat1^-/- and rag2^-/- stat1^-/- mice were diseased, their TG were beginning to hemorrhage, and the animals were within 24 hours of death. Clinically, herpesviral latency refers to the absence of infectious virus and disease in a host, and requires the repression of viral replication in the entire population of virus-infected cells in the body. Thus, although a small subset of neurons represses HSV-1 replication in a Stat 1-independent manner, we conclude that both Stat 1 and lymphocytes are required for a latent HSV-1 infection to be established at the clinically relevant level of the host organism.

ICP0^- viruses are rapidly repressed in scid or rag2^-/- mice. The failure of ICP0^- viruses to sustain replication in lymphocyte-deficient mice is not due to a leaky adaptive immune response, nor is this a non-specific phenotype of any HSV-1 mutant. For example, VP16^- and thymidine kinase^- mutants of HSV-1 establish slowly progressing infections that are fatal in scid mice 2829. Rather, it appears that an innate host response that is dependent upon the IFN-α/β receptor and Stat 1 potently represses the replication of ICP0^- viruses in scid or rag2^-/- mice.

It is generally believed that ICP0 functions as an activator in the balance between HSV-1 latency and reactivation, and that ICP0 acts to prevent host cells from repressing HSV-1 replication in vitro 5630. However, a credible explanation has been lacking to explain how synthesis of ICP0, or lack thereof, can globally effect whether or not HSV-1 initiates productive viral replication. The results of the current study suggest a potentially simple explanation: when ICP0 is synthesized, the protein overcomes Stat 1-dependent cellular repression of HSV-1 such that the virus can complete its replication cycle; when ICP0 is not synthesized, Stat 1-dependent cellular repression silences the HSV-1 genome.

Given ICP0's capacity to function as a viral IFN antagonist in vitro 9101415, we infer that ICP0 most likely functions in virus-infected cells to prevent an IFN-inducible block to viral replication (Checkpoint 1, Fig. 1C). Although PML has been proposed to serve a critical role in repressing ICP0^- viruses 21, our findings in PML^-/- mice do not support a direct role for PML in the process by which host cells repress ICP0^- viruses in vivo. Therefore, it remains to be determined how ICP0 antagonizes Stat 1-dependent repression of HSV-1.

The available evidence suggests that the 9.3 kb R_L region, which contains the ICP0 gene, plays a pivotal role in regulating HSV-1 latency (Fig. 4). The evidence that supports this point is summarized: 1. Synthesis of ICP0 promotes HSV-1 reactivation in latently infected TG neurons 2431; 2. The antisense LAT gene is the only viral gene transcribed during latency and produces stable 1.5 and 2.0 kb introns that accumulate in neurons 3233; 3. Synthesis of ICP34.5 is necessary for HSV-1 to complete its replication cycle in neurons 343536; and 4. De-repression of the antisense L/ST gene produces an HSV-1 mutant that overexpresses L/ST RNA and is replication-impaired in neurons despite an intact ICP34.5 open-reading frame 3.

Failure to synthesize the R_L-encoded viral IFN antagonists, ICP0 or ICP34.5, is a possible mechanism by which HSV-1 may cease productive replication in vivo (Fig. 1C). Failure to synthesize ICP0 renders HSV-1 vulnerable to repression by the host IFN response. Likewise, failure to synthesize ICP34.5 renders HSV-1 vulnerable to innate immune repression in scid mice 37, and this repression is dependent on IFN-α/β receptors and PKR 11. If synthesis of ICP0 and ICP34.5 is necessary to promote HSV-1 replication in vivo, then it is possible that the LAT and L/ST genes provide HSV-1 with an efficient mechanism to pro-actively halt the synthesis of ICP0 or ICP34.5, such that HSV-1 can rapidly cease productive replication in vivo.

This hypothesis may seem counterintuitive. Why would HSV-1 carry two genes that promote the IFN-induced repression of the viral genome? Therefore, it is worth noting that herpesviruses are presented with two choices at the height of an immune response: 1. cytolytic destruction of the host cell by CD8⁺ T cells, or 2. cessation of viral antigen synthesis before CD8⁺ T cells can destroy the host cell. Given the arrangement of genes in the R_L region (Fig. 4), it is possible that the LAT and/or L/ST genes may serve to ensure the timely shutoff of HSV-1 replication and antigen synthesis when the local immune response exceeds a critical threshold in an HSV-1 infected tissue. This hypothesis may be relevant in explaining: i. how viral antigen synthesis decreases from high to undetectable levels within 8 hours after T cells infiltrate HSV-1 infected ganglia 38; ii. how HSV-1 infected neurons avoid cytolytic destruction by T cells 3940; and iii. why neurons infected with LAT^- viruses undergo apoptosis (cytolysis?) more frequently than neurons infected with LAT⁺ viruses 414243. Given the dearth of evidence that the LAT or L/ST genes actually antagonize the synthesis of ICP0 and ICP34.5, further work will be required to address these hypotheses.

HSV-1 becomes hypersensitive to IFN-inducible repression in the absence of ICP0 or ICP34.5 16. Given the extensive similarity between HSV-1 and HSV-2, the results suggest a logical approach to develop a live, attenuated vaccine against genital herpes. When introduced at the peripheral epithelium, ICP0^- viruses are incapable of producing disease in lymphocyte-deficient scid or rag2^-/- mice. Despite their avirulence, ICP0^- viruses establish latent infections in the ganglia that innervate the site of inoculation (Fig. 8) and elicit an adaptive immune response that protects immunocompetent mice against virulent HSV-1 (Fig. 9). In contrast, a replication-defective ICP4^- virus fails to elicit any semblance of protective immunity.

The advantage of a live, attenuated HSV vaccine would be two-fold: 1. the vaccine strain would be antigenically identical to wild-type HSV with the exception of a single mutated open-reading frame (e.g., ICP0), and 2. the latent infection established by such a virus might boost the adaptive immune response over time through periodic synthesis of viral antigen at the single cell level 4445. Failure to sustain a broad-based immune response over time likely accounts for the failure of subunit vaccines or replication-defective viruses to protect against genital herpes 4647. While a live HSV vaccine is often considered unsafe because the vaccine strain would establish a latent infection in the recipient, such claims ignore the natural history of HSV infections 4849. About 4 billion people currently harbor latent HSV-1 and/or HSV-2 in their nervous system, and ~3 billion of these infections are asymptomatic. Further investigation will be required to determine if ICP0^- strains of HSV-2 can be used to achieve an appropriate balance between safety and antigenicity in developing a live, attenuated vaccine against genital herpes.

Vero cells and L7 cells 52 were propagated in Dulbecco's Modified Eagle's medium (DMEM) containing 0.15% HCO₃^- supplemented with 5% fetal bovine serum (FBS), penicillin G (100 U/ml), and streptomycin (100 mg/ml), hereafter referred to as "complete DMEM." Wild-type HSV-1 strains KOS, KOS-GFP, and McKrae were propagated in Vero cells cultured in complete DMEM. The ICP0^- virus n212 5354 contains a 14 bp insertion (C

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KOS-GFP is a recombinant virus derived from HSV-1 strain KOS that expresses green fluorescent protein (GFP) from a CMV promoter-GFP gene cassette inserted in the intergenic region at the 3' ends of the UL26 and UL27 genes, which converge from opposite strands of DNA 56. To avoid disrupting the 3' untranslated region of either the UL26 or UL27 gene, the intergenic region (Kpn I – Fsp I; 52,733 – 53,150) was duplicated and the CMV-GFP cassette is inserted between these duplicated sequences. No known open-reading frames are disrupted by this 2.0 kbp insertion into the HSV-1 genome.

The ICP0^- virus 0^--GFP was constructed from a chimeric ICP0-GFP gene 57, in which the GFP open-reading frame was inserted into a Xho I restriction site in codon 105 of the ICP0 gene (Fig. 4). This mutation was transferred into HSV-1 strain KOS by homologous recombination. Southern blot analysis demonstrated that 0^--GFP possessed the desired mutation in both copies of the R_L region (Fig. 4). Northern blot analysis confirmed that 0^--GFP expresses an ICP0 mRNA that migrates at ~3.1 kb as opposed to the wild-type 2.4 kb ICP0 mRNA transcribed from KOS. 0^--GFP is phenotypically identical to the well-established ICP0^- null virus n212 5354, based on the following observations: 1. Both n212 and 0^--GFP are repressed with identical kinetics in rag2^-/- mice; 2. Both n212 and 0^--GFP are virulent in stat1^-/- mice; 3. Both n212 and 0^--GFP are hypersensitive to IFN-α/β in vitro 14; 4. Only 1% of n212 and 0^--GFP virions form plaques on Vero cells 5458; 5. ICP0, but not ICP4 or VP16, provided in trans from adenovirus vectors allow n212 and 0^--GFP to efficiently form plaques on Vero cells 31, and the efficiency of plaque formation is the same as observed on ICP0-complementing L7 cells 143152.

Female mice of the strains specified in Table 1 were inoculated with HSV-1 at 6- to 10-weeks of age and were handled in accordance with the NIH Guide for the Care and Use of Laboratory Animals. BALB/c mice, BALB/c scid mice, and IFN-γ receptor null (ifngr^-/-) mice were obtained from the Jackson Laboratory (Bar Harbor, ME). Strain 129 mice, rag2^-/- mice, stat1^-/- mice, and rag2^-/- stat1^-/- mice were obtained from Taconic Farms (Germantown, NY). PML^-/- mice were obtained from the National Cancer Institute (Frederick, MD). IFN-α/β receptor-knockout (ifnar^-/-) mice and ifnar^-/- ifngr^-/- mice were obtained from B & K Universal Ltd. (East Yorkshire, United Kingdom). Rag2^-/- ifnar^-/- mice were generously provided by Nicole Meissner and Allen Harmsen (Montana State University, Bozeman). Prior to viral inoculation, mice were anesthetized by i.p. administration of xylazine (7 mg/kg) and ketamine (100 mg/kg). Mice were inoculated by scarifying the cornea with a 26-gauge needle and by placing 4 μl complete DMEM containing 2 × 10⁵ pfu of virus on each eye.

Viral titers in the ocular tear film of mice were determined at times after inoculation by swabbing the ocular surface of both eyes with a cotton-tipped applicator, and transferring the tip into 0.4 ml complete DMEM. Viral titers were determined by a 96-well plate plaque assay on the appropriate cell line cultured in complete DMEM containing 0.5% methlycellulose (i.e., Vero cells for wild-type HSV-1, L7 cells for ICP0^- mutants, and E5 cells for ICP4^- mutants).

Titers of infectious virus in homogenates of whole eyes, trigeminal ganglia (TG), or hindbrain were determined by homogenizing tissues in 0.5 ml complete DMEM with a Pro 200 homogenizer (Pro Scientific, Oxford, CT), removing cell debris via centrifugation, and titering 10-fold serial dilutions of clarified supernatant on 24-well plates containing Vero or L7 cells. For all plaque assays, virus-infected cells were cultured in complete DMEM containing 0.5% methlycellulose for two to three days before staining with 20% methanol and 0.5% crystal violet.

GFP fluorescence in eyes, TG, and brains of mice infected with KOS-GFP was visualized on a Nikon TE2000 inverted fluorescent microscope (Nikon Instruments, Lewisville, TX) using a Jenoptik ProgRes C¹⁰⁺ Digital Camera (JenOptik Laser, Jena, Germany). Images were collected at 2× or 4× magnification using identical exposure conditions within a given comparison group, and composite images of the brain were created by stitching together photographs that covered the ventral surface of the brain using the graphics editor, Paint Shop Pro (Jasc Software, Eden Prairie, MN). GFP fluorescence in the eyes of living mice was obtained by placing anaesthetized mice on a petri dish on the stage of the microscope.

Cultures of Vero cells were established in 12-well plates at a density of 2 × 10⁵ cells per well and were cultured in complete DMEM. Cells were treated 24 hours later by replacing the culture medium with complete DMEM containing no IFN or 200 U/ml IFN-β (PBL Biomedical Laboratories, Piscataway, NJ). Sixteen hours later, Vero cells were inoculated with 2.5 pfu per cell of wild-type HSV-1 strain KOS, 0^--GFP, or the ICP4^- virus n12. After allowing 45 minutes for adsorption, the inoculum was replaced with complete DMEM containing no IFN or 200 U/ml IFN-β. Cultures were harvested 4.5, 9.0, 13.5, or 18.0 hours after inoculation by transfer to a -80°C freezer. Upon thawing, viral titers were determined by plaque assay on appropriate indicator cells.

Mice were euthanized and perfused with 0.1 M phosphate-buffered saline (PBS) followed by 4% paraformaldehyde. The six TG from each group of mice were immersed in 4% paraformaldehyde for 1 hour, equilibrated with 30% sucrose, embedded in Tissue-Tek^® O.C.T. compound (Sakura Finetechnical, Tokyo, Japan), and frozen in liquid nitrogen. Each block of tissue containing the six TG from one group of mice was cut into ~160 sections of 7 μM thickness. Slides were processed to label 1. LAT⁺ neurons with rhodamine, and 2. HSV antigen⁺ neurons with fluorescenin, as follows. LAT riboprobes, specific for bases 119629–119975 of HSV-1, were prepared at 37°C with digoxigenin RNA Labeling Mix (Roche, Mannheim, Germany). LAT riboprobe synthesis reactions were treated with DNase I and filtered through a Sephadex G-50 column prior to use. Tissue sections were incubated in a pre-hybridization buffer, and then hybridized to LAT-specific riboprobes by an overnight 45°C incubation. Tissue was washed with 2× SSC, treated with 20 μg/ml of RNase A in 2× SSC at 37°C, followed by serial washes in 0.5× SSC and 0.1× SSC. Tissue sections were equilibrated with 0.1 M PBS, and were sequentially incubated with 1. rhodamine-labeled anti-digoxigenin Fab fragments, 2. 3% normal rabbit serum, and 3. fluorescein-labeled rabbit polyclonal anti-HSV antibodies (DAKO Cytomation, Carpinteria, CA) diluted 1:100 in 1% normal rabbit serum. Fluorescent-labeled tissue sections were then washed with 0.1 M PBS, and mounted under cover slips.

Latently infected mice were sacrificed on days 38 and 39 p.i., TG were aseptically removed, and each TG was placed in one well of a 24-well plate containing 1 ml of complete DMEM. Once TG were harvested for an entire 24-well plate, the TG were heat stressed by transfer to a 43°C, 5% CO₂ incubator for 3 hours. After heat stress, explants were transferred to a 37°C, 5% CO₂ incubator. Twenty-four hours later, TG and cell culture medium were transferred to 24-well plates seeded 6 h earlier with 5 × 10⁴ L7 cells per well in a volume of 0.5 ml of complete DMEM. On days 6 and 10 after explantation, TG were transferred to a 24-well plate containing freshly seeded L7 cells. After 14 days in culture, TG explants were homogenized in 500 μl of complete DMEM with a Pro 200 homogenizer (Pro Scientific) and TG homogenates were transferred to a -80°C freezer. Freeze-thawed TG homogenates were centrifuged to remove tissue debris, and 200 μl of clarified supernatant was used to inoculated each well of a 12-well plate of L7 cells (1 × 10⁵ cells per well). After allowing 45 minutes for viral adsorption, the viral inoculum was aspirated, the cell monolayer was rinsed with 1 ml complete DMEM, and the rinse solution was replaced with 1 ml complete DMEM. Monolayers of L7 cells treated with TG homogenates were observed for six days for the development of viral cytopathic effects.

The left and right TG from each mouse were placed into a single 1.5 ml microfuge tube, and transferred to -80°C until the time of DNA extraction. DNA was isolated by a standard phenol: chloroform extraction procedure 59, and the number of HSV-1 genomes per TG was determined by a competitive PCR assay, which is described as follows.

The oligonucleotide primers used in the competitive PCR assay, VP16-a (5'-GGACTCGTATTCCAGCTTCAC-3') and VP16-b (5'-CGTCCTCGCCGTCTAAGTG-3'), amplified a 260-bp fragment of the HSV-1 VP16 gene. To provide an internal control for each PCR assay, a VP16 competitor template was generated by the method of Siebert and Larrick 60. In brief, an irrelevant sequence from pUC18 was amplified with the primers VP16 mimic-a (5'-GGACTCGTATTCCAGCTTCAC

GGAGGACCGAAGGAG

CCAGTGCTGCAATGA

PCR assays were conducted as follows: A solution containing 1× Taq buffer, 50 μM of each dNTP, 0.25 μM of each VP16 primer, 5% glycerol, and ~1500 copies of VP16 competitor template per 50 μl reaction was prepared. Forty-two microliters of this master mix were placed in 0.65 ml tubes and overlaid with mineral oil, and 100 ng of TG DNA (3 μl), DNA standards, or negative control DNA sample was added to each tube. The tubes were heated to 90°C in a thermal cycler, and 2.5 U of Taq polymerase diluted in 5 μl of Taq buffer was added to each sample. PCR samples were incubated for 35 cycles of 94°C for 1 minute 15 seconds, 59.5°C for 1 minute 15 seconds, and 72°C for 40 seconds.

Measurement of VP16 gene and competitor PCR product yields was performed by a modification of the dot blot procedure of Hill et al. 61. From each amplified PCR sample, 15 μl was diluted in 500 μl of a 0.4 M NaOH solution, transferred to a 0.6 ml microfuge tube, heated to 90°C for 10 minutes, snap-cooled on ice, and blotted on Zeta Probe GT nylon membrane (BioRad Laboratories, Hercules, CA) in an 8 × 12 dotblot pattern using a Convertible™ vacuum filtration manifold (Whatman-Biometra, Gröningen, Germany). Two identical dotblots of each set of PCR samples were produced and crosslinked with 0.2 J/cm² in a UV crosslinker (Spectronics Corporation, Westbury, NY). One dotblot was hybridized to a radiolabeled oligonucleotide specific for VP16 (5'-GTCGTCGTCCGGGAGATCGAGCAGGCCCTC-3'), and the second duplicate dotblot was hybridized to a radiolabeled oligonucleotide specific for the competitor PCR product (5'-CGCTCGTCGTTTGGTATGGCTTCATTCAGC-3'). Both oligonucleotide probes were end-labeled with [α-³²P] dATP using terminal deoxynucleotidyl transferase (Promega Corporation, Madison, WI). Each probe was allowed 16 h to hybridize to a membrane at 42°C in a solution containing 20 ng/ml labeled probe, 7% SDS, 120 mM NaH₂PO₄, and 250 mM NaCl. Excess probe was removed from membranes by sequential rinses in 0.1× standard saline citrate (SSC) containing 0.1% SDS and membranes were exposed to phosphor screens, which were scanned on a Cyclone PhosphorImager (Perkin Elmer Life Sciences, Boston, MA). The amount of radolabeleled probe hybridized to each dotblot was determined using OptiQuant v4.0 software (Perkin Elmer Life Sciences).

A two-fold dilution series of VP16 plasmid DNA defined the relationship between the copy number of VP16 genes in each PCR (x) and the logarithm of the ratio of VP16 to competitor PCR product yields (y) amplified from each DNA sample. The standard curve was described by the equation x = arctanh (y), but for convenience Microsoft Excel's trendline-fitting feature was used to rapidly define a third order polynomial equation (x = ay³ + by² + cy + d) that approximates the sigmoid shape described by this equation (Fig. 8B). To estimate the copy number of viral genomes per TG, the number of HSV-1 genomes per PCR was multiplied times 150. This derivation is based on the fact that an average of 15 μg of DNA is extracted from each TG pair using an extraction procedure that recovers 50% of the total DNA. Thus, ~15 μg of DNA was present in a single TG, and the input of 0.1 μg of TG DNA per PCR contains ~1/150^th (0.1 μg out of 15 μg) of the total number of HSV-1 genomes present in a single TG.

Analysis of numerical data was performed with the software packages Microsoft Excel and Modstat (Modern Microcomputers, Mechanicsville, VA). All viral titers were transformed by adding a value of 1 to the number of plaque-forming units detected per sample, such that all data could be analyzed on a logarithmic scale. The significance of differences between multiple groups was evaluated by one-way analysis of variance followed by a post hoc t-test. The significance of differences between strain 129 mice, rag2^-/- mice, stat1^-/- mice, versus rag2^-/- stat1^-/- mice was evaluated by two-way analysis of variance. The goodness of fit of the standard curve used in the competitive PCR assay was determined by regression analysis using the method of least squares.