Plant and animal genomes have thousands of genes that encode non-protein-coding (nc) RNAs. While recent attention has focused significantly on small interfering RNAs (siRNAs) and micro RNAs (miRNAs), ncRNAs also include rRNA, tRNA, small nuclear (sn) RNA, small nucleolar (sno) RNAs, and some of the lesser-known RNAs such as vault RNAs, Y RNAs, rasi-RNAs and piRNAs [reviewed in 1]. It is now recognized that only 2% of the human genome encodes for protein-coding RNAs while 60 to 70% of our DNA is transcribed into ncRNAs 23. Hence, despite accumulating research on siRNA, miRNA and piRNA, we are likely at the tip of the iceberg in our understanding about functions and regulatory roles served by ncRNAs in cellular metabolism, pathogenesis and host-pathogen interaction.

A key biological process served by small ncRNAs is a phenomenon termed RNA interference (RNAi). Recent reviews have reprised the discovery of RNAi and summarized the current state of knowledge about this process 45. In brief, a central tenet of RNAi posits that small guide RNAs recruit, in a sequence-complementary manner, a multi-protein complex composed in part of RNA-binding proteins to RNA targets. This large multi-protein RNAi complex has been shown to include members of the Argonaute ribonuclease III protein family; and depending on biological context, the complex has been found to effect post-transcriptional gene silencing (PTGS), transcriptional gene silencing (TGS), and/or co-transcriptional gene silencing (CTGS) (Fig. 1, top).

Conventional wisdom suggests that biological processes are balanced by two principles, yin and yang, which oppose one another in their actions to confer equilibrium. For example, the cell-proliferative effects of oncogenes are countered by commensurate provocations of cellular senescence and apoptosis 678. Moreover, frequently, potent transcriptional activators are also equally strong repressors 910. Indeed, recent developments raise that the yin (negative) of RNAi may be shadowed by a yang (positive). Thus, some cellular 11121314 and viral 15 studies now suggest that a rarely-glimpsed face of "RNAi" may visualize activation rather than repression. Verily, RNA sequence-mediated positive regulation could exist more prevalently than currently acknowledged since, in principle, there is no reason why other RNA-binding proteins different from Argonaute-related members cannot be similarly tagged and guided by ncRNAs (Figure 1, bottom).

Biological studies on mammalian viruses have illuminated aspects of gene regulation by small non-coding RNAs and their RNA-binding proteins. Early results from the HIV-1 TAR RNA and its binding protein Tat framed a platform for how a small non-coding RNA and a viral RNA-binding protein cooperate to up modulate gene expression 1617. Indeed, although unrecognized at the time, the first human cellular protein identified to bind TAR RNA, TRBP 1819 presaged a clue to the mechanistic process of RNAi. Hence, fourteen years after the initial characterization of its binding to TAR, TRBP was revealed as a crucial component of the mammalian miRNA processing machinery 202122. Consistent with TRBP's role in miRNA-processing, a recent report demonstrated that TAR RNA in human cells is engaged by TRBP and processed by the RNAse III Dicer protein into a miRNA guide 23.

The above sequence of events not withstanding and although the RNAi machinery is preserved intact in mammalian cells and mammalian RNAi machinery can be instructed to target invading viruses in therapeutic settings, there is a school of thought that mammals do not use ncRNA/RNAi to regulate viral infections 24. This view is partly rationalized by the argument that mammals have a surfeit of other means to defeat effectively viral pathologies; thus an intact mammalian RNAi machinery is not needed, and cells have extinguished this mechanism as it applies to viral infection 24. Confoundingly, that annually 3 million human deaths arise from HIV-infection alone 25 and ~20% of all human cancers are caused by viral infections 26 indicate that mammalian defenses are not nearly so replete that effective antiviral pathway(s) should become extinct.

Recent experimental findings are, in fact, consistent with physiological use by mammalian cells of small ncRNA/RNAi to regulate viruses. First, three studies have converged to illustrate that small ncRNAs (siRNAs and piRNAs) are used in human and mouse cells to suppress the replication of endogenous retroviruses (i.e. retrotransposons) 272829. Second, bioinformatics and experimental results persuasively imply that mammalian viruses including HIV-1 are targeted by discrete human miRNAs 3031323334. Third, repression of mammalian Dicer enzyme was found to up regulate cellular replication of HIV-1 and vesicular stomatitis virus (VSV) 353637. One straightforward interpretation of the latter finding, which does not exclude others, is that the unrepressed mammalian Dicer-RNAi pathway normally acts to moderate HIV-1 and VSV replication. Finally, a KSHV viral miRNA (miR-K12-11) was identified as a viral orthologue of human miR-155 38. To the extent that miR-K12-11 targets KSHV- and cellular- sequences, then cellular miR-155 can be reasoned to act upon the same KSHV-sequence regulated by miR-K12-11. Indeed, pending the clarification of additional details, extant observations are consistent with the employment of ncRNAs by mammalian viruses to regulate viral functions, by mammalian cells to regulate cellular functions, by mammalian viruses to regulate cellular functions, and by mammalian cells to regulate viral functions (Figure 2).

If cells restrict viruses with non-coding RNAs, do viruses respond with countermeasures? In principle, viral counter stratagems could include a) protection from restriction, b) suppression of restriction; c) evasion from restriction; d) modulation of restriction profiles, and e) adaptation to restriction.

We have outlined in brief two views on virus- cell ncRNA interaction in mammals. The first view embraces a null hypothesis --- virus-cell ncRNA interactions exist in lower eukaryotes but not physiologically in mammals 24. While this view may be correct, several lines of evidence discussed here point against its limitations. A second view is that the RNAi machinery exists in mammalian cells not just for artificial siRNA-exploits but as a physiological mechanism used by cells and viruses to regulate viral and cellular functions (Figure 2). Subsumed within this view is the thesis that RNAi is a part of the armamentarium used by mammalian cells to regulate, perhaps positively and negatively in context-specific fashion, the replication of endogenous and exogenous viruses. We anticipate that more time and further investigation will be needed to validate the accuracy of the one or the other view point.

If small ncRNAs are used in mammalian cells to regulate cellular and viral functions, then one could venture several predictions. First, many more (cellular and viral) RNA-binding proteins that adapt small RNAs to mediate both negative and positive gene regulation will be revealed. Second, mammalian viruses will be shown to encode a variety of ncRNAs that have regulatory roles. Some future examples might mirror the HTLV-1 regulatory HBZ ncRNA 57; others may emerge from the processing of antisense HIV-1 and HTLV-1 transcripts 585960; and even others may behave like TAR or RRE. Third, additional viral RNA-binding proteins (perhaps Rev and Rex) will be shown to have setting-specific RNAi- modulatory properties, and many viruses will be found to extensively reshape cellular miRNA expression profiles.

Since its first description a relatively short period of time ago, 9868 papers have already been published on RNAi (data from Pubmed search using the term, RNAi). An additional prediction (which will almost certainly be correct) is that RNA-guided gene regulation will continue to hold many exciting and unexpected scientific findings which will be published profusely in the coming years.