Acupuncture is an essential component of Traditional Chinese Medicine and is finding increased application in therapeutic concepts for treating various illnesses. The most important effective structural elements are acupoints which are divided topographically into specific meridians. Many references regarding the qualitative characteristics of acupoints are available and histological tests seem to prove this for a number of acupoints 1. Attempts to visualize the course of "energetic paths" in acupuncture using physical-technical methods, are described by different authors. However, the results from investigations available up until now are controversial and generally accepted proof for meridians cannot be considered as being given 2345.

One goal of this study was to examine whether possible stimulation-induced meridian-like structures, as recently described by other authors 2, can be visualized and objectified using modern biomedical engineering methods of thermography 6, simultaneously at different infrared wavelength ranges.

We could not visualize structures which could be connected with possible meridians, as described in Traditional Chinese Medicine, in any of our volunteers. We were able to clearly objectify and quantify technical reflection artifacts; however, indications for a biological correlation could not be found (Tab. 1). In the cases when only needle or laserneedle (685 nm) stimulation was used no thermal reflection phenomena were present and also no meridians could be detected (compare Tab. 1).

Figure 2 shows the reflection (mirror-like) artifacts which result from reflection and scattering, in a 30-year-old volunteer. According to the position of the moxa-cigar (circular white area) in dependency with the camera position, reflections showing an optical linear path on the thermogram, can be applied to optional areas of the body.

In this manner, typical artifacts from each volunteer can be determined in the "thermographic pictures". Figure 3 shows a further example for this phenomenon. Here, simultaneous measurements in two different wavelength ranges were made using both infrared cameras. While the first camera (7.5 – 13 μm; Fig. 3a) yielded less reflection artifacts with an all over better optical resolution, the second camera (2 – 5 μm) revealed more distinct artifacts. The energy maximum of the moxa-cigar lies in the range of 3 – 3.5 μm. Since the radiation curve markedly flattens at 7.5 – 13 μm obviously greater reflection occurs within the range of 2 – 5 μm.

Figure 4 demonstrates the occurrence of reflection phenomena in a 35-year-old volunteer at the thigh. Simultaneously, surface skin temperature and flux were determined with a combined temperature and microcirculatory sensor which was applied to the skin. The reflection artifacts at the area of the sensor led to false temperature representation of 38.5°C at the sensor surface (smooth plastic surface) in the thermograms from both cameras. The neighboring skin surface revealed a 4.2°C higher temperature (35.4°C instead of 31.2°C) in the thermograms. The taped on temperature sensor confirmed the correct value of 31.2°C for surface skin temperature at this point.

Figure 5 shows the structures which were registered on a black carton (Fig. 5 left). Due to the optical characteristics in the infrared range, the marked (linear type) structure after external stimulation with an infrared heat source is visible in the thermogram.

Thermic and infrared energy lies within a wavelength range no longer detectable by the human eye. This energy lies within the region of the electro-magnetic spectrum which is perceived as being warm. Contrary to visible light, every object in this region with a temperature over absolute zero radiates heat. When the temperature of an object is higher, the intensity of emitted infrared radiation is also higher. Infrared cameras produce pictures of invisible infrared- or heat radiation, thus enable exact temperature measurements.

This technique of temperature measurement has already been used in several studies dealing with acupuncture research 23456. Advantages of this method are the high local resolution as well as optical data registration without requiring skin contact. In addition, the method is passive, i.e. no energy is transmitted into the body and thus is completely harmless. This allows longer examination times and therefore can also be used on infants 17.

Dynamic changes in temperature distribution, as possible during acupuncture can be registered well and reliably 6. Areas which produce less heat or are less perfused are shown as much cooler on the calculated pictures as those with better circulation. Differences in temperature which are less than 0.08°C can be objectified. An important factor is the emission coefficient of the skin, which lies at 0.97 in this study. This factor is dependent upon the direction of radiation. For this reason, border regions are visible as being much cooler. The misrepresentation of the measurement value resulting from geometrics of the radiated structures must be considered during interpretation 7. Errors can also occur because of false emission coefficients at the skin because e.g. greasy skin creams can lead to an obvious reduction in the emission coefficient 6.

Our experience shows that material characteristics such as reflection or refraction, two important examples in this study, are dependent upon the wavelength of optical radiation, and can show strong differences in the visible spectral range. Thermal radiation spreads straight out in a homogenous medium and is influenced by border areas. For example, when air borders on a smooth metal surface, reflection occurs. An example for directed reflection is the surface mirror. Ideal diffuse reflection can be achieved with bright white finishes and coarse surfaces. On most border surfaces, mixed types of reflection occur. Further, part of the radiation is absorbed by the surface.

Reflection is very important, particularly in thermography. In the majority reflection is unwanted in the radiation path of optical instruments and should be minimized. Often, background rays mask the measurement signal due to the reflection in irregular surfaces of the measurement object including the human body. This effect partially determines the limited applicability of infrared cameras. It is important to note, that the optical laws are valid for the entire spectral range; obvious deviations are evident at the borders of extreme ultraviolet or extreme infrared radiation 7.

The technical, but obviously not biological reflection phenomena described in this study can be applied to any desired area of the body. "Thermic pictures" with partially well known characteristics in the temperature range between 20°C and 40°C above the filmed body region can be made. The fact that we are here dealing with technical artifacts and not biological phenomena can be justified by the following:

1. Reflections can be applied to optional parts of the body depending on the position of the moxa-cigar in connection with the angle of incidence of both cameras. In addition, the structure and form of phenomena (circular, linear) in dependence with the values described above can be varied. Thus, correspondence with the meridian structures according to Traditional Chinese Medicine is not given (compare Fig. 2,3,4).

2. The moxa-cigar radiates more than 550°C with maximum energy ranging between 3 and 3.5 μm. The radiation curve flattens markedly between 7.5 and 13 μm, thus, stronger reflections in the range of 2 – 5 μm occur than in the long-wave infrared range. This could be illustrated for the first time using two infrared cameras (compare Fig. 3).

3. Reflection phenomena (artifacts) are also reproducible in both wavelength ranges in non-vital structures such as bed linens or paper (compare Fig. 5).

4. Simultaneous measurement of surface temperature and microcirculation did not show any changes in regard to temperature and circulation. On the contrary, temperature differences up to 7°C could be registered between both methods at the examined regions of the body which underlines the probability of an artifact (compare Fig. 4).

5. So-called moxa-induced meridian paths can be projected (reflected) horizontally or diagonally at any angle (compare Fig. 2).

6. Stronger reflections at both wavelength ranges (2 – 5 μm and 7.5 – 13 μm) can be triggered by using an infrared stimulation device.

7. No path equivalent meridians on the body surface could be visualized in any volunteer during acupuncture stimulation (manual needle acupuncture and laserneedle stimulation as well as moxibustion).

In conclusion, we note that thermographic methods such as infrared cameras at wavelength ranges of 2 – 5 μm and 7.5 – 13 μm and other High-Tech methods 89101112131415161819202122232425 are effective complementary methods in acupuncture research which support demystification of this treatment method. However, the validity of the method for proving meridian structures according to the view of Traditional Chinese Medicine, must be considered critically and analysed scientifically. In a publication which attempted to visualize meridians and their path using infrared thermography, the authors 2 described that the left stomach meridian could be visualized during stimulation with a moxa-cigar near the left foot. At the same time, the right spleen meridian should be visualized on the right foot. Based on the foundations of Traditional Chinese Medicine, it is improbable that exactly these two meridians can be activated simultaneously. According to current technical standings, the visualization of energetic paths in the sense of meridians as described in recent literature is not possible using thermography. On the contrary, the supposed thermographic reproduction of meridians is dependent upon physical-technical artifacts caused by thermic reflections.

According to current technical standings and to the method proposed by other authors 2, the visualization of energetic paths in the sense of meridians seems to be not possible using thermography. Further scientific studies are required regarding the possible visualization of meridians.