Similarly to the berry development of V. vinifera varieties, the development of Norton berries is characterized by a two-stage growth pattern. Sugar accumulation began at the early stages and accelerated during véraison. Also following the pattern of V. vinifera berry development, the levels of titratable acidity dropped at stage 34 (at 66 days after bloom [DAB]) and continued to decrease until the berry was ripe. The descriptors of berry development, including berry diameter, titratable acidity and soluble solids, are presented in an accompanying paper (Ali et al., in preparation). We started sampling on June 26, 2008 when the skin could be separated from the pulp of the berry. At this point, the berry was at stage 31 (17 DAB) on the Eichorn-Lorenz phenological scale. Subsequently, skin samples were taken at stages 33, 34, 35, 36, 37 and 38, corresponding to 37, 66, 71 (véraison), 85, 99, and 127 DAB. Skin tissue was frozen in liquid nitrogen and total RNA was extracted subsequently. The RNA was then labeled and hybridized to GRAPEGEN Affymetrix GeneChips. Processing of raw intensity values in CEL files and subsequent normalization and Median polishing were described in the paper (Ali et al., in preparation).

A Principal Component Analysis (PCA) of the eighteen arrays was performed to assess the similarity of expression values among the replicates (Additional File 1). The results from the PCA indicated a high degree of similarity among three biological replicates that were clustered tightly within the scatterplot. In addition, PCA showed that data of two proximal developmental stages were more similar to each other than data of distal developmental stages. There is a clear alignment and separation of developmental stages along the PC1 in the plot (Additional File 1). The eighteen sets of the data were then converted to z-scores and subjected to two-way unsupervised agglomerative cluster analysis (Additional File 2). This analysis showed that each stage represents a major branch which contains only the three biological replicate data for that stage. The results from these two analyses demonstrated that there is a good reproducibility among the three biological replicates and thus all data were included in the analysis. Pearson correlation coefficients between biological replicates were also calculated and were in the range of 0.9812 to 0.9976 (Additional File 3), further corroborating significant correlations between biological replicates in each developmental stage.

After the data of all eighteen arrays were processed and assessed for quality, the error-weighted intensity experiment definitions (EDs) were calculated by averaging the intensity of three biological replicates for each stage and then error-corrected using the Rosetta error model 31 . ANOVA was conducted on the error-weighted intensity of three biological replicates at each stage across six developmental stages with the Benjamini-Hochberg False Discovery Rate multiple test correction 32 . This resulted in the discovery of 15,823 probe sets that exhibited significant variations at the transcript levels between at least two developmental stages at P ≤ 0.001 (Additional File 4). The differentially expressed probe sets comprise more than 78% of all probe sets on the microarray, indicating that a large number of genes represented on the array changed significantly at transcript levels at some points during berry development. To discover the genes whose transcript levels varied significantly from a baseline calculated from all six developmental stages, the intensity EDs of each probe set were divided by an error-weighted average of all six developmental stages. Under the criteria of absolute fold-change ≥2.0 in at least one developmental stage and having a LogRatio P -value ≤ 0.001 in at least one stage, we identified 3,352 probe sets (Additional File 5). We selected this group of the most significantly expressed genes for the subsequent analysis. The large number of transcripts that changed at expression levels corroborated earlier findings that genes of different functions were detected in the berry skin at the beginning of véraison and the later stages of ripening, reflecting the dramatic biochemical changes that take place during berry ripening 7 15 .

We used the nucleotide sequence from which each set of probes was designed to acquire the best-matched GSVIVT ID in Genoscope (http://www.genoscope.cns.fr/externe/GenomeBrowser/Vitis/) or TC number in DFCI Grape Gene Index (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=grape). The total of 3,352 probe sets represented 2,760 unique genes. We removed those probe sets where more than one probe set was assigned to the same GSVIVT ID or TC numbers but showed different expression patterns, and compiled them into a separate file for future analysis. At this time, it is not possible to discern what factors, such as alternatively spliced transcripts or degradation biases of the 5'-end and 3'-end portion of mRNA, influence the expression levels of these genes. We subjected the Log₂-transformed fold-change of the remaining 2,359 unigenes to clustering by the k-means method. A total of 20 clusters were defined from this group of genes based on the figure of merit value (Additional File 6).

Transcript abundance of these genes in cluster 1, 12, 13, 18 and 20 increased after véraison (Figure 1). These five clusters contained a total of 1,053 genes. Cluster 11 (113 genes) and Cluster 16 (42 genes) represented a pattern of transient increase and decrease, respectively, of transcript levels at the onset of véraison and subsequently unchanged post-véraison. The expression pattern of cluster 8 (65 genes) and cluster 19 (60 genes) was reciprocal. In cluster 8, transcript levels increased pre-véraison and decreased post-véraison. In cluster 19, transcript levels decreased at véraison, but increased both pre-véraison and post-véraison. The remaining eleven clusters included 1,026 genes and exhibited a pattern of steady decline post-véraison. The genes in each cluster are listed in Additional File 6.

A total of 48 differentially expressed genes were associated with defense, disease resistance, and hypersensitive response (Table 1). Among them, twenty one genes were up-regulated, and twenty five genes were down-regulated post-véraison. These defense-related genes include the well characterized polygalacturonase inhibiting protein (PGIP), dirigent protein, NBS-LRR, Non-race-specific disease resistance 1 (NDR1), powdery mildew resistant 5 (PMR5), and harpin-induced protein 1 genes.

Especially noticeable is the expression profile of the PR-1 gene, which is an indicator for the induction of local defense and systemic acquired resistance (SAR) in plants 33 34 . In grapevine, the PR-1 gene (

GSVIVT00038581001)

Five probe sets were annotated as thaumatin-like proteins and two as osmotins. Their transcript levels increased significantly in the late stages of Norton berry development (Additional File 5 and 6), as was shown previously in varieties of V. vinifera 7 29 . Thaumatin-like proteins inhibit spore germination and hyphal growth of E. necator, Phomopsis viticola, and Botrytis cinerea 40 . We found that transcript levels of five chitinase genes increased post-véraison in Norton berry skin (cluster 12, 13, 19, and 20). Transcript levels of basic class I (VCHIT1b) and a class III (VCH3) chitinase of grapevines increase in response to the chemical activators of SAR and are considered as markers of SAR 41 . Furthermore, enzymatic activities of chitinase and ß-1,3-glucanase also increase during berry development in the absence of pathogens 15 . Non-specific lipid transfer proteins (nsLTPs) belong to a family of small cystein-rich proteins that are induced in response to fungal elicitors and are associated with grapevine defense 42 43 44 . A possible LTP-jasmonic acid complex may protect grape berries against B. cinerea 42 . Transcripts of one probe set (

GSVIVT00037486001

The cis- and trans-piceid compounds of the stilbene family constitute a major group of phytoalexins in grapevines that are involved in the defense responses to pathogens 45 . They have been shown to have antifungal activities against several fungal pathogens including Plasmopara viticola 46 and B. cinerea 47 48 . They also exhibit antibacterial activity against Xylella fastidiosa 49 , the pathogen of Pierce's disease on grapevine. In addition, stilbenic compounds possess anticancer and anti-inflammatory activities that have potential benefits to human health 50 . Stilbene synthase (STS) is the key enzyme that catalyzes the formation of 3', 4', 5'-trihydroxystilbene (resveratrol) via the condensation of one 4-coumaroyl-CoA and three malonyl-CoA molecules (Figure 2A). This condensation reaction represents a branch point in the phenylpropanoid pathway, at which CHS channels 4-coumaroyl-CoA molecules towards flavonoid synthesis and STS towards stilbene synthesis.

Grape berry skin is the main tissue where the synthesis of stilbenes occurs 51 . STS was found to be localized mostly in the cell wall of hypodermal cells in the exocarp, which is in agreement with the detection of stilbenic compounds mainly in the exocarp during berry development 51 . It was also demonstrated that stilbenic compounds and transcripts of the key genes PAL, 4CL, and STS accumulated progressively in ripening berries of Pinot Noir 52 and Corvina 53 . The composition of stilbenic compounds differs significantly among grape varieties. Mature berries of Pinot Noir contain the highest levels of stilbenes, while the stilbene content of Cabernet Sauvignon berries is ranked 41st among 48 red-skinned grapes 52 . There is a high correlation between the transcript levels of PAL, 4CL, and STS and the abundance of stilbenic compounds in grape varieties 52 53 . We found that six of the ten paralogous STS genes on the GrapeGen Chip are grouped into clusters 18 and 20, and the transcripts of these genes increased steadily and significantly post-véraison (Figure 1). Interestingly, PAL and 4CL were also found in clusters 18 and 20, in which transcripts of these genes significantly increased in the final two stages (Figure 1). Highly coordinated expression of PAL, 4CL, and STS post-véraison strongly supports the conclusion that the stilbene biosynthesis pathway is up-regulated during the development of Norton berry skin. In our previous microarray analysis of the pathogen-induced transcriptome in grapevines, we discovered that STS genes were strongly induced in response to PM infection 26 . These results confirm that stilbenes, together with other phytoalexins and defense-related proteins, are part of the defense weaponry for protecting berries from pathogen attacks. This defense strategy appears to be developmentally regulated in Norton berry skin.

Results of previous microarray analyses of tissue-specific transcriptomes demonstrated that the majority of genes encoding enzymes in the biosynthesis of flavonoids, lignin, anthocyanins and proanthocyanidins were expressed preferentially in the berry skin of grapevine 9 . These genes include PAL, C4H, and 4CL, encoding key enzymes which catalyze the first three steps of the phenylpropanoid pathway (Figure 2A). The present microarray analysis also showed that transcripts of three PAL genes and one 4CL gene increased significantly in Norton berry skin post-véraison (Table 2). The increasing levels of PAL and 4CL transcripts most likely led to higher accumulation of the substrate 4-coumaryl-CoA for the down-stream pathways. This trend coordinates well with the transcriptional regulation of chalcone synthase (CHS) (

GSVIVT00037967001),

GSVIVT00014584001

GSVIVT00036313001)

GSVIVT00001063001)

GSVIVT00014047001).

After we had compared the previous microarray analysis of Cabernet Sauvignon berry development 7 with the present results in Norton (Table 2), we discovered that the two grape varieties share eight genes that are differentially expressed in the flavonoid pathway. Particularly interesting is the finding that transcripts of F3H (

GSVIVT00036784001

GSVIVT00015347001

GSVIVT00037967001)

The differential expression of flavonoid biosynthesis genes in Norton berry skin development prompted us to compare the transcript abundance of the most relevant genes in Norton with those in Cabernet Sauvignon. We conducted qPCR assays to compare transcript levels of eleven genes between the two varieties (Additional File 7). We chose these eleven genes based on their key roles in the pathway that F3'H, F3'5'H-1a and -2a, DFR, LDOX, and UFGT are involved in biosynthesis of anthocyanins while ANR and LAR1/2 catalyze PA synthesis (Figure 2B). Expression of the eleven genes exhibited distinctive patterns between the two varieties (Figure 3).

Transcripts of F3'H, F3'5'H1a and F3'5'H2a reached maximum levels at 99 DAB in Norton, and were significantly higher in Norton than in Cabernet Sauvignon post-véraison. Transcripts of DFR increased to the highest levels at véraison in both varieties, and then declined sharply in Cabernet Sauvignon, but remained at the same levels throughout the ripening stages in Norton. Transcripts of LDOX were very low in Cabernet Sauvignon, but in Norton they increased to a peak at 85 DAB, declined at 99 DAB, and then bounced back to the same levels at 127 DAB as at 85 DAB. UFGT transcript levels reached a maximum at 99 DAB, and also were significantly higher in Norton than in Cabernet Sauvignon (Figure 3).

Transcripts of ANR attained peak levels at véraison, and declined gradually in Norton, but were significantly higher in Norton than in Cabernet Sauvignon post-véraison. Transcripts of LAR1 were the most abundant at véraison, significantly higher in Cabernet Sauvignon than in Norton, and then declined to be barely detectable in the final two stages in Cabernet Sauvignon. In Norton, LAR1 transcript levels increased steadily after 85 DAB. On the other hand, LAR2 transcripts increased, and were also more abundant in Norton than in Cabernet Sauvignon post-véraison (Figure 3).

Taken together, transcripts of all eleven genes accumulated more abundantly in Norton after véraison, suggesting that the biosynthesis of flavonoid compounds remains highly activated in the skin of Norton berries post-véraison.

In plants, GSTs consist of a large, complex gene family and play important roles in anthocyanin transport to or storage in the vacuole 54 . They conjugate the tripeptide glutathione to a variety of electrophilic compounds, thus limiting damaging effects of reactive oxygen species 55 56 . RNA-seq analysis showed that transcripts of 64 of the predicted 87 GSTs in grapevine were detected during berry development of the grape variety 'Corvina' 57 . However, the specific roles of the individual GSTs were not clear. Four GST isoforms were identified in cell suspension cultures of grapevine. Two of them were highly expressed and involved in anthocyanin accumulation or transport into the vacuole 58 . One grapevine GST (

GSVIVT00023496001

The methylation of phenolic compounds, as catalyzed by O-methyltransferases (OMTs), is an important step in flavonoid metabolism 59 . For example, caffeoyl CoA and caffeic acid OMTs are able to methylate lignin precursors 60 61 . On the basis of substrate specificity and function in stabilizing phenolic products, plant OMTs have been classified into various categories. Increasing evidence suggests that the expression of OMT genes is correlated with the accumulation of methylated anthocyanins in grapevines 62 63 64 . The qPCR results show that one OMT (

GSVIVT00002831001

To investigate transcriptional regulation of the flavonoid pathway during berry skin development, we analyzed the transcript levels of six genes encoding MYB transcription factors (MYBA1, MYBA2, MYBPA1, MYBPA2, MYB5A and MYB5B) by qPCR (Additional File 7). All transcription factor genes assayed were expressed at some stages of berry skin development, but the expression patterns of some of them were distinct between the two varieties (Figure 4).

Expression profiles of MYBA1 and MYBA2 are very similar between the two varieties. MYBA1 transcripts reached peak levels at 85 DAB after véraison in Norton and then declined and remained low. Similarly, the transcripts of MYBA1 reached the highest level at 59 DAB (véraison) and decreased gradually post-véraison in Cabernet Sauvignon. MYBA2 transcripts also reached the highest level at 59 DAB, and then decreased until 112 DAB in Cabernet Sauvignon. In contrast, in Norton MYBA2 transcripts reached the highest level at 99 DAB.

The transcript profiles of MYB5A and MYB5B were similar during all of berry skin development, with high levels at véraison in both varieties. MYB5A transcript levels are slightly higher in Norton than in Cabernet Sauvignon while transcript levels of MYB5B are higher at all developmental stages in Cabernet Sauvignon than in Norton. The transcripts of MYBPA1 in Norton increased sharply from 66 to 71 DAB (véraison), reached the highest level at 85 DAB, and then declined to a barely detectable level. The transcript levels of MYBPA1 in Cabernet Sauvignon, on the other hand, remained low throughout berry development. In contrast, MYBPA2 transcripts reached maximum levels at 71 DAB in Cabernet Sauvignon, while they remained steadily low in Norton throughout berry development. The results suggest that MYBPA1 may play a more prominent role in Norton than in Cabernet Sauvignon whereas MYBPA2 in Cabernet Sauvignon than in Norton in the regulation of PA biosynthesis. The variety-specific regulation of MYBPAs warrants further functional analysis of their regulatory elements.

To match gene expression patterns with flavonoid profiles, we analyzed the accumulation of the flavan-3-ols catechin, epicatechin, epigallocatechin (EGC), and epicatechin gallate (ECG) in berry skin across seven developmental stages (Figure 5). Norton and Cabernet Sauvignon have comparative levels of catechin at 17 DAB. In Cabernet Sauvignon, catechin levels remained high until just after véraison, whereas in Norton, catechin dropped to the lowest levels at 71 DAB (véraison) and then rose until 127 DAB. Epicatechin was not detected in either variety until véraison, but was detectable in Norton at 85 and 99 DAB as well as in Cabernet Sauvignon post-véraison. EGC levels remained steady in Cabernet Sauvignon throughout berry development, but increased steadily in Norton until 127 DAB. ECG was detected only in Cabernet Sauvignon (data not shown).

We analyzed the accumulation profiles of five anthocyanin derivatives (cyanidin-, peonidin-, delphinidin-, petunidin- and malvidin-monoglucoside/diglucoside) at four post-véraison stages of berry skin for both varieties by high performance liquid chromatography (HPLC) (Figure 6). Accumulation patterns of the five anthocyanins in Cabernet Sauvignon berry skin in the present study are remarkably similar to the previous observations in Cabernet Sauvignon under different climate and environmental conditions 65 . The accumulation of the five anthocyanins begins at véraison, and leads to much higher levels in Norton than in Cabernet Sauvignon at harvest (Figure 6).

In agreement with previous results that diglucoside derivatives of anthocyanins are found in Vitis species of North American origin 66 , anthocyanin-diglucosides are highly abundant and contribute a major portion to the total anthocyanin content in Norton berry skin (Figure 6). Interestingly, the amounts of monoglucoside derivatives of malvidin and peonidin are not significantly different between Norton at 127 DAB, and Cabernet Sauvignon at 112 DAB. Diglucoside derivatives of peonidin and malvidin accumulated to significantly higher levels than their respective monoglucoside derivatives in Norton (Figure 6). Malvidin-diglucoside is the major anthocyanin in Norton while malvidin-monoglucoside contributes primarily to anthocyanin in Cabernet Sauvignon. The five anthocyanin derivatives reached their highest levels in Cabernet Sauvignon after véraison and remained steady until 112 DAB; whereas in Norton they continued to increase steadily until harvest at 127 DAB.

The differences detected in the accumulation of cyanidin-, peonidin-, delphinidin-, petunidin- and malvidin derivatives prompted us to compare anthocyanin profiles of ripe Norton and Cabernet Sauvignon berry skin in detail. We used liquid chromatography-tandem mass spectrometry (LC-TIS/MS/MS) to identify the anthocyanin compounds. Thirty five different anthocyanins were identified in the two grape varieties (Table 3 and Figure 7). Eight of the 35 compounds were common to both varieties; sixteen of them were detected only in Norton. Norton-specific compounds include those previously described 3'-5' diglucoside derivatives as well as a number of sophoriside-glucosides and p-coumaryl-glucosides. Rutinoside derivatives appear to be unique to Cabernet Sauvignon. Cabernet Sauvignon had a single diglucoside anthocyanin, namely petunidin 3',7'-diglucoside (Table 3).

At 127 DAB, anthocyanin diglucosides contribute 59% of the total anthocyanins in Norton berry skin. The major anthocyanins are malvidin derivatives that contribute 49% (5.73 mg/g FW) to total anthocyanins, followed by delphinidin (17%), petunidin (11%), peonidin (12%), and cyanidin (7%). In Cabernet Sauvignon, the main anthocyanin component is malvidin-3'-glucoside, which contributes 67% (1.82 mg/g FW) to the total anthocyanin amount at 112 DAB, followed by peonidin (14%), delphinidin (10%), petunidin (6.8%) and cyanidin (2%). Overall, in harvest-ripe berries, the total anthocyanin content in Norton berry skin (11.59 mg/g FW) is considerably higher than in Cabernet Sauvignon berry skin (2.70 mg/g FW).

A concise summary of coordinated transcription of key genes and biosynthesis of anthocyanins and PAs in the developing berry skin is presented in Figure 8. Transcript levels of F3'H and F3'5'H1a/2a peaked at 99 DAB and were higher in Norton than in Cabernet Sauvignon (Figure 3). We speculate that more flavonoid precursors (dihydroflavonols) are produced that are converted to anthocyanins and PAs in Norton than in Cabernet Sauvignon. This speculation is supported by the patterns and levels of accumulation of anthocyanins and PAs during berry development of the two varieties (Figure 5 and 6). One DFR gene (

GSVIVT00014584001

LDOX catalyzes the last two steps of anthocyanin synthesis (Figure 2B). The transcriptional profile of one LDOX gene (

GSVIVT00001063001)

GSVIVT00005344001)

UFGT catalyzes the last step in the anthocyanin biosynthesis pathway (Figure 2B). MYBA1 and MYBA2 regulate the transcription of UFGT 21 67 68 . Transcript levels of MYBA1/A2 peaked at véraison (59 DAB) in Cabernet Sauvignon, and post véraison at 85 and 99 DAB in Norton. Correspondingly, transcripts of one UFGT gene (

GSVIVT00014047001

Berries from V. vinifera 'Cabernet Sauvignon' and V. aestivalis 'Norton' were collected at six developmental stages during the 2008 growing season from vines grown in a vineyard in the Missouri State Fruit Experiment Station, Mountain Grove, Missouri, USA, according to the phenological developmental stages defined by Coombe 69 . The berries were sampled at the following stages: 31 (pea-sized), 33 (still hard), 34 (softening), 35 (véraison), followed by 36, 37 and 38 (harvest ripe). Berry skin was separated from pulp, and pulp tissues were further removed by rubbing the internal side of the skin against filter paper. The cleaned skin tissues were immediately frozen in liquid nitrogen and stored at -80°C.

Total RNA was extracted from the skin tissue according to the procedure of Reid et al. 70 , using a CTAB-spermidine extraction buffer. Total RNA was treated with 1 unit of DNase I (Ambion, Austin, Texas, USA) for 30 minutes at 37°C and purified using RNeasy MinElute Cleanup kit (Qiagen, Valencia, California, USA). RNA quantity and quality were assessed by Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California, USA). For cDNA synthesis, two μg of total RNA was reverse transcribed with oligo-dT in a 20 μl reaction mixture using the MultiScribe reverse transcriptase (Applied Biosystems, Branchburg, New Jersey, USA) according to the manufacturer's instructions.

Array hybridization was performed at the DNA Core Facility, University of Missouri (Columbia, Missouri). A total of 0.5 μg of total RNA was used to make the biotin-labeled antisense RNA (aRNA) target using the MessageAmp™ Premier RNA amplification kit (Ambion, Austin, Texas) following the manufacturer's protocol. Briefly, total RNA was reverse transcribed to first strand cDNA with an oligo(dT) primer bearing a 5'-T7 promoter using ArrayScript reverse transcriptase. First strand cDNA then underwent second-strand synthesis to convert it into double stranded cDNA as a template for in vitro transcription. The biotin-labeled aRNA was synthesized using T7 RNA transcriptase with biotin-NTP mix. After purification, the aRNA was fragmented in 1× fragmentation buffer at 94°C for 35 min. One hundred and thirty μL of hybridization solution containing 50 ng/μl of fragmented aRNA was hybridized to the Affymetrix GRAPEGEN GeneChip (Affymetrix, Santa Clara, California) at 45°C for 20 hrs. After hybridization, the chips were washed and stained with R-phycoerythrin-streptavidin in an Affymetrix fluidics station 450 using fluidics protocol Midi_euk2v3-450. The image data were acquired by Affymetrix GeneChip scanner 3000 and Affymetrix GCOS software.

The Affymetrix microarray (GRAPEGEN GeneChip) used in this analysis included probe sets for 23,096 unigenes 30 . The intensity data of all genes on the microarray were analyzed by ANOVA with the Benjamini-Hochberg False Discovery Rate Multiple Test Correction method and applying a p-value of 0.001. The resulting data set was further reduced by applying a cut-off fold change of 2 or greater, which led to a final set of 3,352 significantly changed probe sets.

To annotate the putative function of the 3,352 probe sets that exhibited significant expression changes during berry development, the FASTA sequences were BLAT-searched against the 8× genomic sequences of V. vinifera PN40024 (http://www.genoscope.cns.fr/externe/GenomeBrowser/Vitis/) by using each FASTA sequence as query to acquire a Genoscope ID number. If no Genoscope ID was found for the query sequence, a Tentative Consensus (TC) ID was retrieved from VVGI5 database (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=grape). The latest annotations for all Genoscope IDs and relational Network IDs, InterPro domain IDs, Gene Ontology IDs, UniProtIDs, TCs and functions have been published (Table S1, 71 ), and were used as the reference for functional category and annotation. The original annotations by the GeneChip manufacturing group were also cross-referenced for verification.

More than one sequence was annotated with the identical Genoscope or DFCI ID in 401 cases, which brought the total number of unigenes down to 2,760. All genes with multiple annotations and four sequences for which neither a Genoscope annotation nor a DFCI match were found were removed from the data set, resulting in 2,359 unigenes.

The expression profiles of the 2,359 unigenes were clustered using the k-means method with Pearson's correlation as distance. They were grouped into 20 clusters after evaluation of the Figure of Merit (FOM) graph in the Multiple Experiment Viewer version 4.4 software package.

Transcript levels in grape skin were measured by quantitative real-time PCR, using SYBR Green in the MX3005P system (Stratagene) following the manufacturer's manual. The reaction mixture (20 μl, in triplicate) contained 0.5 μl 1:10 diluted cDNA as a template and 20 pmole each of the forward and reverse primers specific to each gene. The primers were designed from the 3'-UTR region to avoid any unspecific amplification. Thermal cycling conditions were as follows: 95°C for 10 min, 65 cycles of 95°C for 15 sec, 60°C for 30 sec and 1 cycle of 95°C for 1 min, 60°C for 30 sec and 95°C for 30 sec. The annealing temperature (60°C) was determined computationally when designing the primer. The melt curves for the products of these assays produced a single peak, indicating that a single gene had been amplified. The specificity of each primer pair was also checked by gel electrophoresis and by sequencing the PCR products and comparing them with the sequence of the target gene. PCR efficiency (E) was calculated from the exponential phase of each individual amplification plot and the equation (1 + E) = 10^slope based on a previous method 72 . Expression levels of genes of interest (GOI) were normalized to that of ACTIN by dividing the C_T value of GOI by the C_T value of ACTIN. Gene expression was expressed as mean and standard error calculated based on three biological replicates.

For anthocyanin extraction, frozen berry skin tissue was ground in liquid nitrogen, and 500 mg of the ground tissue was extracted with 5 mL acidified methanol (60% (V/V) methanol containing 0.1% (w/V) ascorbic acid) for 24 hours on a shaker in the dark at room temperature. The extracts were centrifuged twice at 16,100 g for 10 minutes. The final supernatants were kept in the dark and refrigerated until analysis; two samples were prepared from each biological replicate.

For proanthocyanidin extraction, frozen seeds or frozen berry skin were ground in liquid nitrogen, and 500 mg of ground tissue was used for extraction in 5 ml extraction buffer (70% [V/V] acetone containing 0.1% [w/V] ascorbic acid) for 24 hr at room temperature on a rotating shaker in darkness. The water phase was separated from the acetone phase by adding sodium chloride to saturation. After removal of the acetone phase, the water phase was extracted with additional sodium chloride-saturated 100% acetone, and the resulting acetone phase was combined with the first acetone phase. The samples were dried under a stream of nitrogen, the pellet re-dissolved in 750 μL of 60% methanol acidified with 0.1% ascorbic acid, centrifuged at 16,100 g for 10 minutes, and the final supernatant kept in darkness and under refrigeration until analysis; two samples were prepared from each biological replicate.

Anthocyanin and proanthocyanidin content and composition were determined by reverse-phase HPLC using an HP1100 series (Agilent) Chemstation, with a Zorbax Eclipse XDB-C18 (80 Angstrom, 4.6 × 150 mm, particle size 3 μm) column with a guard column. The binary solvent system of solvent A (acetonitrile (HPLC grade, EMD Chemicals, USA) and Solvent B (2% phosphoric acid [(HPLC grade, Sigma Aldrich), V/V Millipore water] was used for both the anthocyanin and the proanthocyanidin analyses. The gradient used for anthocyanin separation was as follows: acetonitrile 6% for 3 min; 8% for 24.50 min; 10% for 22.50 min; 18% for 23.50 min; 90% for 4.5 min; and 8% for 7 min; with a flow rate of 0.8 mL/min for 36 minutes, then 0.6 mL/min for 49 min. The gradient used for proanthocyanidin separation was as follows: acetonitrile 8% for 5 min, 12% for 12 min, 20% for 10 min, 25% for 6 min, 50% for 2 min, 80% for 7 min, 8% for 5 min; with a flow rate of 0.5 mL/min. In each case, the column was maintained at 40°C and the diode array detector was used to record absorption at 280 nm, 335 nm and 520 nm. Malvidin-3-glucoside chloride, catechin hydrate, epicatechin, epicatechin gallate, epigallocatechin, epigallocatechin gallate and proanthocyanidin B2 (all HPLC grade, Sigma-Aldrich) were used to create standard absorption curves. All anthocyanins were expressed as malvidin glucoside equivalents based on the peak areas recorded at 520 nm with a molecular weight correction factor applied. The peak areas recorded at 280 nm in conjunction with the respective standard absorption curves were used to express the proanthocyanidins as mg per gram of fresh weight.

Anthocyanins were extracted by following the protocol for extracting proanthocyanidins as described in the previous section. All samples were analyzed using a 4000 QTRAP LC-TIS-MS-MS system (Applied Biosystems, Forest City, CA) by monitoring the enhanced product ion (EPI) and multiple reaction monitoring (MRM) in the positive ionization mode. Separation of (10 μL) samples was achieved by using a Gemini-NX C18 HPLC column (Phenomenex, 5 μm, 150 mm × 2 mm) combined with a C18 guard column (Phenomenex, 4 mm × 2 mm). The mobile phase flow was set to 0.45 mL/min with binary gradient elution, using solvent A (aqueous 5% formic acid solution) and B (95% CH₃CN, 5% formic acid). The gradient was as follows: 0-3 min, 5% B; 3-15 min, 5-9% B; 15-27 min, 9-13.5% B; 27-32 min, 13.5% B, 32-42 min, 13.5-18.5% B; 42-44 min, 18.5% B; 44-51 min, 18.5-22.5% B; 51-55 min, 22.5-30% B; 55-56 min, 30-40% B; 56-60 min, 40-70%; 60-60.1 min, 70-100% B; 60.1-70 min, 100% B; 70.0-70.1 min, 100-5% B; 70.1-80 min, 5% B. The elution of anthocyanins was monitored at 520 nm. The following TIS source parameters were used: CUR 30 eV, CAD high, IS 5500, TEM 550°C, DP 40 eV, CE 10 eV. The mass scan range was 50 to 1000. For anthocyanin quantification, five anthocyanin standards (Chloride salt of delphinidin (Sigma, MO), cyanidin (Chromadex, CA), petunidin (Chromadex, CA), peonidin (Chromadex, CA) and malvidin (Chromadex, CA) were used to create a calibration curve for each anthocyanin. All calibration curves were linear, with R²≥0.998.