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1.

3772
Accesses

Research article   Open Access Highly Accessed

Effect of experimental treatment on GAPDH mRNA expression as a housekeeping gene in human diploid fibroblasts

Azalina Zainuddin, Kien Chua, Norhazira Rahim, Suzana Makpol BMC Molecular Biology 2010, 11:59 (14 August 2010)

Abstract | Full text | PDF | PubMed

2.

1761
Accesses

Methodology article   Open Access Highly Accessed

The RIN: an RNA integrity number for assigning integrity values to RNA measurements

Andreas Schroeder, Odilo Mueller, Susanne Stocker, Ruediger Salowsky, Michael Leiber, Marcus Gassmann, Samar Lightfoot, Wolfram Menzel, Martin Granzow, Thomas Ragg BMC Molecular Biology 2006, 7:3 (31 January 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

3.

1737
Accesses

Research article   Open Access Highly Accessed

Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1

Rita Schwaiger, Christoph Schwarz, Katarina Furtwängler, Valery Tarasov, Andy Wende, Dieter Oesterhelt BMC Molecular Biology 2010, 11:40 (28 May 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

In the salt-loving archaeon Halobacterium salinarum R1, the leucine-responsive regulatory protein Lrp affects global transcription of genes involved in central metabolism and amino acid synthesis, while LrpA1 specifically regulates the aspartate transaminase gene aspB3.

4.

1301
Accesses

Research article   Open Access Highly Accessed

G-quadruplex recognition activities of E. Coli MutS

Edward A Ehrat, Bradley R Johnson, Jonathan D Williams, Glen M Borchert, Erik D Larson BMC Molecular Biology 2012, 13:23 (2 July 2012)

Abstract | Full text | PDF | PubMed

5.

1289
Accesses

Research article   Open Access Highly Accessed

Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis

Hualiang He, Mei Cheng, Xiao Yang, Jinxiu Meng, Ai He, Xiaoying Zheng, Zhuoya Li, Pengjuan Guo, Zhihua Pan, Ximei Zhan BMC Molecular Biology 2009, 10:97 (25 October 2009)

Abstract | Full text | PDF | PubMed

6.

1176
Accesses

Research article   Open Access Highly Accessed

Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus

Sascha Hibbeler, Joern P Scharsack, Sven Becker BMC Molecular Biology 2008, 9:18 (29 January 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

7.

761
Accesses

Research article   Open Access Highly Accessed

Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Asad Maroufi, Erik Van Bockstaele, Marc De Loose BMC Molecular Biology 2010, 11:15 (15 February 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

8.

719
Accesses

Research article   Open Access

High throughput nano-liter RT-qPCR to classify soil contamination using a soil arthropod

Muriel E de Boer, Sandra Berg, Martijn JTN Timmermans, Johan T den Dunnen, Nico M van Straalen, Jacintha Ellers, Dick Roelofs BMC Molecular Biology 2011, 12:11 (1 March 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

9.

687
Accesses

Methodology article   Open Access Highly Accessed

A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector

Raquel Buj, Noa Iglesias, Anna M Planas, Tomàs Santalucía BMC Molecular Biology 2013, 14:18 (20 August 2013)

Abstract | Full text | PDF | ePUB | PubMed

10.

659
Accesses

Research article   Open Access Highly Accessed

Cloning, ligand-binding, and temporal expression of ecdysteroid receptors in the diamondback moth, Plutella xylostella

Baozhen Tang, Wei Dong, Pei Liang, Xuguo Zhou, Xiwu Gao BMC Molecular Biology 2012, 13:32 (19 October 2012)

Abstract | Full text | PDF | PubMed

11.

620
Accesses

Research article   Open Access

RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation

Marta Wanarska, Beata Krawczyk, Piotr Hildebrandt, Józef Kur BMC Molecular Biology 2011, 12:17 (22 April 2011)

Abstract | Full text | PDF | PubMed

12.

618
Accesses

Research article   Open Access

Molecular cloning and RNA interference-mediated functional characterization of a Halloween gene spook in the white-backed planthopper Sogatella furcifera

Shuang Jia, Pin-Jun Wan, Li-Tao Zhou, Li-Li Mu, Guo-Qing Li BMC Molecular Biology 2013, 14:19 (4 September 2013)

Abstract | Full text | PDF | ePUB | PubMed

13.

597
Accesses

Methodology article   Open Access Highly Accessed

Optimal use of tandem biotin and V5 tags in ChIP assays

Katarzyna E Kolodziej, Farzin Pourfarzad, Ernie de Boer, Sanja Krpic, Frank Grosveld, John Strouboulis BMC Molecular Biology 2009, 10:6 (5 February 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

14.

582
Accesses

Review   Open Access

VprBP (DCAF1): a promiscuous substrate recognition subunit that incorporates into both RING-family CRL4 and HECT-family EDD/UBR5 E3 ubiquitin ligases

Tadashi Nakagawa, Koushik Mondal, Patrick C Swanson BMC Molecular Biology 2013, 14:22 (13 September 2013)

Abstract | Full text | PDF | ePUB | PubMed

15.

580
Accesses

Methodology article   Open Access Highly Accessed

Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR

Nicholas Silver, Steve Best, Jie Jiang, Swee Thein BMC Molecular Biology 2006, 7:33 (6 October 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

16.

563
Accesses

Review   Open Access Highly Accessed

Human single-stranded DNA binding proteins are essential for maintaining genomic stability

Nicholas W Ashton, Emma Bolderson, Liza Cubeddu, Kenneth J O’Byrne, Derek J Richard BMC Molecular Biology 2013, 14:9 (1 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

17.

562
Accesses

Research article   Open Access Highly Accessed

Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions

Carla F Barsalobres-Cavallari, Fábio E Severino, Mirian P Maluf, Ivan G Maia BMC Molecular Biology 2009, 10:1 (6 January 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

18.

558
Accesses

Methodology article   Open Access Highly Accessed

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR

Valeria Valente, Silvia A Teixeira, Luciano Neder, Oswaldo K Okamoto, Sueli M Oba-Shinjo, Suely KN Marie, Carlos A Scrideli, Maria L Paçó-Larson, Carlos G Carlotti BMC Molecular Biology 2009, 10:17 (3 March 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

19.

475
Accesses

Methodology article   Open Access

Normalisation genes for expression analyses in the brown alga model Ectocarpus siliculosus

Aude Le Bail, Simon M Dittami, Pierre-Olivier de Franco, Sylvie Rousvoal, Mark J Cock, Thierry Tonon, Bénédicte Charrier BMC Molecular Biology 2008, 9:75 (18 August 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

20.

473
Accesses

Methodology article   Open Access Highly Accessed

Single cell transcriptomic analysis of prostate cancer cells

Christopher J Welty, Ilsa Coleman, Roger Coleman, Bryce Lakely, Jing Xia, Shu Chen, Roman Gulati, Sandy R Larson, Paul H Lange, Bruce Montgomery, Peter S Nelson, Robert L Vessella, Colm Morrissey BMC Molecular Biology 2013, 14:6 (16 February 2013)

Abstract | Full text | PDF | PubMed

21.

457
Accesses

Research article   Open Access

Pseudo attP sites in favor of transgene integration and expression in cultured porcine cells identified by streptomyces phage phiC31 integrase

Yanzhen Bi, Ximei Liu, Long Zhang, Changwei Shao, Zhuo Ma, Zaidong Hua, Liping Zhang, Li Li, Wenjun Hua, Hongwei Xiao, Qingxin Wei, Xinmin Zheng BMC Molecular Biology 2013, 14:20 (8 September 2013)

Abstract | Full text | PDF | ePUB | PubMed

22.

442
Accesses

Research article   Open Access Highly Accessed

APOBEC3 inhibits DEAD-END function to regulate microRNA activity

Sara Ali, Namrata Karki, Chitralekha Bhattacharya, Rui Zhu, Donna A MacDuff, Mark D Stenglein, April J Schumacher, Zachary L Demorest, Reuben S Harris, Angabin Matin, Sita Aggarwal BMC Molecular Biology 2013, 14:16 (26 July 2013)

Abstract | Full text | PDF | ePUB | PubMed

23.

431
Accesses

Research article   Open Access

Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

Piotr M Skowron, Jolanta Vitkute, Danute Ramanauskaite, Goda Mitkaite, Joanna Jezewska-Frackowiak, Joanna Zebrowska, Agnieszka Zylicz-Stachula, Arvydas Lubys BMC Molecular Biology 2013, 14:17 (6 August 2013)

Abstract | Full text | PDF | PubMed

24.

402
Accesses

Research article   Open Access Highly Accessed

Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

Amy T McCurley, Gloria V Callard BMC Molecular Biology 2008, 9:102 (12 November 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

25.

396
Accesses

Research article   Open Access

Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells

Liesbeth Vossaert, Thomas O’Leary, Christophe Van Neste, Björn Heindryckx, Jo Vandesompele, Petra De Sutter, Dieter Deforce BMC Molecular Biology 2013, 14:21 (12 September 2013)

Abstract | Full text | PDF | ePUB | PubMed

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