Role of retinal non-coding RNA2 in mouse retinal development
The function of several long intergenic noncoding RNAs (lincRNAs) in the nervous system is unclear. In the retina, the most abundant lincRNA is retinal non-coding RNA 2 (RNCR2). To examine how RNCR2 controls cell fate decisions in the developing retina, dominant negative constructs consisting of different domains of the noncoding RNA fused to IRES-GFP were electroporated into retina at postnatal day 0.5. Forced expression of the 5' domain of RNCR2 fused to IRES-GFP caused an increase in the fraction of GFP-positive amacrine cells (visualized in green and red at the middle of the image) seen in the retinal inner nuclear layer. Retina were stained with anti-GFP to visualize electroporated cells (green), anti-syntaxin to visualize amacrine cells (red), and DAPI to visualize nuclei (blue)
Taken from: Seth Blackshaw et al., 2010, BMC Developmental Biology [View article]
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