Role of Optomotor-blind (Omb) in the developing Drosophila epithelial wing disc
To examine how the Decapentaplegic (Dpp) target gene, optomotor-blind (omb), maintains epithelial cell architecture in response to the Dpp anterior/posterior morphogen gradient, the effect of reduced omb expression in the wing imaginal disc was investigated. In the Drosophila wing pouch, clones with strongly reduced level of Omb (visualized by lack of the green anti-Omb staining) induced a reorganization of the epithelial cytoskeleton and caused cell retraction, as shown by F-actin accumulation (red). Central clones tend to round up indicating a difference in cell adhesion relative to the surrounding wild-type cells. Imaginal discs were stained with anti-Omb antiserum (green) and phalloidin stained F-actin (red).
Taken from: Gert Pflugfelder et al., 2010, BMC Developmental Biology [View article]