FIP3 is a cell cycle-regulated phospho-proteinRab11 and its effector molecule Rab11-FIP3 (FIP3) associate with recycling endosomes and traffic into the furrow and midbody of cells during cytokinesis. A functional role for FIP3 was identified by observing FIP3 dephosphorylation and the concomitant change in FIP3 distribution from cytosol to the membrane during the transition from anaphase to telophase. Immunostaining of HeLa cells in early telophase as determined by the re-distribution of highly dynamic FIP3 from a characteristic perientrosomal localisation to the intercellular bridge region as the cells progresses from early to late telophase. Cells were transiently transfected with GFP-FIP3 (pseudo-coloured green) and after 24h fixed and stained with anti-tubulin (pseudo-coloured red).
Taken from: Gwyn Gould et al., 2012, BMC Cell Biology [View article]