Depletion of SM22 protein alters actin dynamicSM22 is an actin-associated protein that may play an important role in cell fate as indicated by its reduced levels in tumour cell lines and increased presence in aging cells. siRNA-mediated depletion of SM22 in REF52 cells was used to investigate the contribution of SM22 to actin dynamics. Reduction in SM22 levels results in a less bundled, less well organized and potentially more dynamic actin cytoskeleton. These changes to actin dynamics may contribute to the tumourigenic properties of cells and their metastatic potential. Immunostaining of actin stress fibres with FITC-phalloidin (green) shows the altered, “orthogonal” actin fibre phenotype in cells expressing the anti-SM22 RNAi (stained with dsRed, red).
Taken from: Steve Winder et al., 2012, BMC Cell Biology [View article]
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