Human collagen beta (1-O) galactosyltransferase, GLT25D1, localizes to the endoplasmic reticulum
Glycosyl transferase 25 domain 1 (GLT25D1) has galactosyltransferase activity towards one of its substrates, mannose binding lectin (MBL), but the subcellular localization of GLT25D1 has yet to be determined. Therefore, human hepatoma cells (Huh7) were co-transfected with MBL fused to GFP (MBL-GFP: green) and full length GLT25D1 tagged with Myc internally (GLT25D1: red). Both have a perinuclear and endoplasmic reticular staining, with substantial co-localization of GLT25D1 with MBL (illustrated by yellow coloring). Another enzyme acting upstream of GLT25D1, lysyl hydroxylases (LH3: blue) shows partial co-localization with GLT25D1 (purple) and MBL (white). Cells were fixed and analyzed for immunofluorescence after 24 h of expression. GFP was visualized directly (green), and GLT25D1 and LH3 were visualized using antibodies against Myc or LH3 (red and blue, respectively).
Taken from: hans van leeuwen et al., 2010, BMC Cell Biology [View article]