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Protein and enzyme technology

This section will consider studies that focus on recombinant proteins and enzymes, cell-free protein expression systems, and biocatalysis using immobilized enzymes.

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1.

Research article   Open Access

Purification and characterization of a novel neutral and heat-tolerant phytase from a newly isolated strain Bacillus nealsonii ZJ0702

Ping Yu, Yirun Chen BMC Biotechnology 2013, 13:78 (28 September 2013)

Abstract | Full text | PDF | PubMed

2.

Methodology article   Open Access

A streamlined implementation of the glutamine synthetase-based protein expression system

Rachel Knox, Joanne E Nettleship, Veronica T Chang, Zhao Kun Hui, Ana Mafalda Santos, Nahid Rahman, Ling-Pei Ho, Raymond J Owens, Simon J Davis BMC Biotechnology 2013, 13:74 (24 September 2013)

Abstract | Provisional PDF | PubMed

3.

Research article   Open Access

Preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase from Pyrococcus furiosus

Bin Li, Zemin Wang, Shiwu Li, William Donelan, Xingli Wang, Taixing Cui, Dongqi Tang BMC Biotechnology 2013, 13:73 (21 September 2013)

Abstract | Provisional PDF | PubMed

4.

Research article   Open Access

An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation

Christian Renz, Nils Johnsson, Thomas Gronemeyer BMC Biotechnology 2013, 13:60 (26 July 2013)

Abstract | Full text | PDF | ePUB | PubMed

5.

Retraction   Open Access Highly Accessed

Retraction: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

Ondrej Kaplan, Karel Bezouska, Ondrej Plihal, Rudiger Ettrich, Natallia Kulik, Ondrej Vanek, Daniel Kavan, Oldrich Benada, Anna Malandra, Ondrej Sveda, Alicja B Vesela, Anna Rinagelova, Kristyna Slamova, Maria Cantarella, Jurgen Felsberg, Jarmila Duskova, Jan Dohnalek, Michael Kotik, Vladimir Kren, Ludmila Martinkova BMC Biotechnology 2013, 13:57 (20 July 2013)

Abstract | Full text | PDF | PubMed | 1 comment

6.

Research article   Open Access

Decolorization applicability of sol–gel matrix immobilized manganese peroxidase produced from an indigenous white rot fungal strain Ganoderma lucidum

Hafiz Muhammad Nasir Iqbal, Muhammad Asgher BMC Biotechnology 2013, 13:56 (13 July 2013)

Abstract | Full text | PDF | PubMed

7.

Research article   Open Access Highly Accessed

Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli

Marina Duarte Lobo, Fredy Davi Silva, Patrícia Gadelha Landim, Paloma da Cruz, Thaís de Brito, Suelen de Medeiros, José Tadeu Oliveira, Ilka Vasconcelos, Humberto Pereira, Thalles Grangeiro BMC Biotechnology 2013, 13:46 (1 June 2013)

Abstract | Full text | PDF | ePUB | PubMed

8.

Research article   Open Access

In situ magnetic separation of antibody fragments from Escherichia coli in complex media

Martin Cerff, Alexander Scholz, Matthias Franzreb, Iris L Batalha, Ana Cecilia A Roque, Clemens Posten BMC Biotechnology 2013, 13:44 (20 May 2013)

Abstract | Full text | PDF | ePUB | PubMed

9.

Research article   Open Access

A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs

Ashley R Long, Catherine C O’Brien, Ketan Malhotra, Christine T Schwall, Arlene D Albert, Anthony Watts, Nathan N Alder BMC Biotechnology 2013, 13:41 (11 May 2013)

Abstract | Full text | PDF | ePUB | PubMed | f1000 |  Editor’s summary

A novel single-step method uses the  styrene-maleic acid copolymer to enable reconstruction of membrane membrane proteins directly into lipid bilayers without requiring the use of detergents that can induce loss of function during the isolation step.

10.

Research article   Open Access

Functional expression of a blood tolerant laccase in Pichia pastoris

Diana M Mate, David Gonzalez-Perez, Roman Kittl, Roland Ludwig, Miguel Alcalde BMC Biotechnology 2013, 13:38 (30 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

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